Q47QX1 (MURD_THEFY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	Tfu_1108

Organism

Thermobifida fusca (strain YX) [Complete proteome] [HAMAP]

Taxonomic identifier

269800 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptosporangineae › Nocardiopsaceae › Thermobifida

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257258
Regions
	Nucleotide binding	116 – 122	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q47QX1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: D790E87EBDECB1D2	FASTA	465	48,595
	10 20 30 40 50 60 MELRGRRVCV TGLGVSGPPV ARALLKHGAH VTVVEGRDDD VNRERARTLR ELGATVELGE 70 80 90 100 110 120 PTTLPAGTDL VVTSPGWRPD APVLVAAADA GVEVIGDVEL AWRLRPAGQV WLAVTGTNGK 130 140 150 160 170 180 TTTVRMLEAM LRADGRRALA VGNVGTPVID AALADGPDRW DILAVELSSF QLHWSATVQP 190 200 210 220 230 240 HAAAIINLAP DHLDWHGGME PYAAAKAKVY GAGTIRIVNA ADPALRELAE THGDPAAPLV 250 260 270 280 290 300 GFSLDVPRAG ELGLVEDLLV DRAFVANPRT EATELATLAD VTPRAPHNVA NALAAAALAR 310 320 330 340 350 360 SIGVAPEAVR TGLRNFQPEP HRIAHVATID GVDYVDDSKA TNAHAAAASL AAYPSVVWIA 370 380 390 400 410 420 GGLLKGADVT GLVRQAASRL RGVVLLGADR HQLREALDTH APHVPVVDVA RTDEGAMAEV 430 440 450 460 VAAAARLAQK GDTVLLAPAA ASMDMFTNYI ERGEAFAREV RALAG

References

[1]

"Genome sequence and analysis of the soil cellulolytic actinomycete Thermobifida fusca YX." Lykidis A., Mavromatis K., Ivanova N., Anderson I., Land M., DiBartolo G., Martinez M., Lapidus A., Lucas S., Copeland A., Richardson P., Wilson D.B., Kyrpides N. J. Bacteriol. 189:2477-2486(2007) [PubMed: 17209016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000088 Genomic DNA. Translation: AAZ55146.1.
RefSeq	YP_289169.1. NC_007333.1.
3D structure databases
ProteinModelPortal	Q47QX1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47QX1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3579290.
GenomeReviews	Gene locus Tfu_1108 in contig CP000088_GR.
KEGG	tfu:Tfu_1108.
NMPDR	fig|269800.4.peg.356.
PATRIC	23902710. VBITheFus33945_1155.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HBG750024.
OMA	ACASWDM.
PhylomeDB	Q47QX1.
ProtClustDB	PRK01438.
Enzyme and pathway databases
BioCyc	TFUS269800:TFU_1108-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_THEFY

Accession

Primary (citable) accession number: Q47QX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program