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Q47QV9

- SYI_THEFY

UniProt

Q47QV9 - SYI_THEFY

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Tfu_1120
Organism
Thermobifida fusca (strain YX)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei611 – 6111ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTFUS269800:GI42-1138-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Tfu_1120
OrganismiThermobifida fusca (strain YX)
Taxonomic identifieri269800 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeNocardiopsaceaeThermobifida
ProteomesiUP000000434: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10601060Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098565Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi269800.Tfu_1120.

Structurei

3D structure databases

ProteinModelPortaliQ47QV9.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi55 – 6511"HIGH" regionUniRule annotation
Add
BLAST
Motifi608 – 6125"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiPRFKTMK.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47QV9-1 [UniParc]FASTAAdd to Basket

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MSKESTRPFP TLPAQIDLPA MEHEVLRRWE EHKIFERSLE QTQGGPTWVF     50
YEGPPTANGT PGVHHVEARV FKDVFPRFKT MKGFYVERKA GWDCHGLPVE 100
VAVEKELGIS GKKDIEAFGI AEFNARCRES VLRNVDAFTE MTRRMGYWVN 150
MDEAYRTMDR EYVESVWWAI KQIWDKGLLV QDYRISPYCP RCGTTLSDHE 200
LAQGYETVTD PSVYVRFPLT SGPLAGQAAL LVWTTTPWTL VSNTAVAVHP 250
EVDYVVATDG SEQLVVAEPL VGAALGDGWT LTGQRFKGAE LERWTYQRPF 300
ELVEFDSPAH FVVLGDYVTV EDGTGLVHQA PAFGADDMQV CRAYGLPVVN 350
PVRNDGTFEE HLDLVGDEFF KTADAALVAD LKDRGLLFKH LDYEHSYPHC 400
WRCHTPLMYY AVPSWYIKTT AIKDQLLAEN AKTNWVPANV KDGRYGEWLR 450
NNVDWALSRS RYWGTPLPIW EFPDGRRICV GSLKELSELS GQDLSDLDPH 500
RPYVDDIVIP DPDADPSLPL EQRVARRVPE VIDVWFDSGA MPFAQWGAPH 550
RNQEKFEANF PAQYICEAID QTRGWFYSLM AVSTLVFGRS SYENVVCLGH 600
ILAEDGRKMS KHLGNILEPI PVMDRHGADA LRWFMAASGS PWMPRRVGHT 650
VLEEIVRKVL LTYYNSASFF TLYAGAGDGW SHEQLADAPA PQDRPLLDRW 700
ILSELHSLIK TVDDALERFD TALAGRALTT FIDDLSNWYV RRSRRRFWAG 750
AGTPEGAAAF ATLFECLETL TLLMAPIVPF ITDHVWQALR RPDAPESVHL 800
ASWPKADESL IDPALSEQMA LVRRLVELGR AARVDSGQRV RQPLARALVG 850
APGFAELPEQ LRAQIAEELN VVQLDPLSVV GGDLVDYSVK PNFRALGKRF 900
GKTTPRVAQA IREADAKTLV ERLRADNAAT VDVDGEQVVL SADEVVVTEQ 950
PREGWTVASE AGETVALDLE LTPELRRAGV AREVIRLVQD ARKSSGLNIS 1000
DRIHLWWSAT DEMTAQAMAE HAEAISSEVL AVSFTEGTGD ADAYEVVSEE 1050
FGITLRLRKA 1060
Length:1,060
Mass (Da):118,881
Last modified:September 13, 2005 - v1
Checksum:i3DA876E2028CE987
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000088 Genomic DNA. Translation: AAZ55158.1.
RefSeqiYP_289181.1. NC_007333.1.

Genome annotation databases

EnsemblBacteriaiAAZ55158; AAZ55158; Tfu_1120.
GeneIDi3579322.
KEGGitfu:Tfu_1120.
PATRICi23902736. VBITheFus33945_1168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000088 Genomic DNA. Translation: AAZ55158.1 .
RefSeqi YP_289181.1. NC_007333.1.

3D structure databases

ProteinModelPortali Q47QV9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269800.Tfu_1120.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ55158 ; AAZ55158 ; Tfu_1120 .
GeneIDi 3579322.
KEGGi tfu:Tfu_1120.
PATRICi 23902736. VBITheFus33945_1168.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi PRFKTMK.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TFUS269800:GI42-1138-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YX.

Entry informationi

Entry nameiSYI_THEFY
AccessioniPrimary (citable) accession number: Q47QV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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