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Q47QV9

- SYI_THEFY

UniProt

Q47QV9 - SYI_THEFY

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermobifida fusca (strain YX)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei611 – 6111ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTFUS269800:GI42-1138-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Tfu_1120
OrganismiThermobifida fusca (strain YX)
Taxonomic identifieri269800 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeNocardiopsaceaeThermobifida
ProteomesiUP000000434: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10601060Isoleucine--tRNA ligasePRO_0000098565Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269800.Tfu_1120.

Structurei

3D structure databases

ProteinModelPortaliQ47QV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi55 – 6511"HIGH" regionAdd
BLAST
Motifi608 – 6125"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiPRFKTMK.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47QV9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKESTRPFP TLPAQIDLPA MEHEVLRRWE EHKIFERSLE QTQGGPTWVF
60 70 80 90 100
YEGPPTANGT PGVHHVEARV FKDVFPRFKT MKGFYVERKA GWDCHGLPVE
110 120 130 140 150
VAVEKELGIS GKKDIEAFGI AEFNARCRES VLRNVDAFTE MTRRMGYWVN
160 170 180 190 200
MDEAYRTMDR EYVESVWWAI KQIWDKGLLV QDYRISPYCP RCGTTLSDHE
210 220 230 240 250
LAQGYETVTD PSVYVRFPLT SGPLAGQAAL LVWTTTPWTL VSNTAVAVHP
260 270 280 290 300
EVDYVVATDG SEQLVVAEPL VGAALGDGWT LTGQRFKGAE LERWTYQRPF
310 320 330 340 350
ELVEFDSPAH FVVLGDYVTV EDGTGLVHQA PAFGADDMQV CRAYGLPVVN
360 370 380 390 400
PVRNDGTFEE HLDLVGDEFF KTADAALVAD LKDRGLLFKH LDYEHSYPHC
410 420 430 440 450
WRCHTPLMYY AVPSWYIKTT AIKDQLLAEN AKTNWVPANV KDGRYGEWLR
460 470 480 490 500
NNVDWALSRS RYWGTPLPIW EFPDGRRICV GSLKELSELS GQDLSDLDPH
510 520 530 540 550
RPYVDDIVIP DPDADPSLPL EQRVARRVPE VIDVWFDSGA MPFAQWGAPH
560 570 580 590 600
RNQEKFEANF PAQYICEAID QTRGWFYSLM AVSTLVFGRS SYENVVCLGH
610 620 630 640 650
ILAEDGRKMS KHLGNILEPI PVMDRHGADA LRWFMAASGS PWMPRRVGHT
660 670 680 690 700
VLEEIVRKVL LTYYNSASFF TLYAGAGDGW SHEQLADAPA PQDRPLLDRW
710 720 730 740 750
ILSELHSLIK TVDDALERFD TALAGRALTT FIDDLSNWYV RRSRRRFWAG
760 770 780 790 800
AGTPEGAAAF ATLFECLETL TLLMAPIVPF ITDHVWQALR RPDAPESVHL
810 820 830 840 850
ASWPKADESL IDPALSEQMA LVRRLVELGR AARVDSGQRV RQPLARALVG
860 870 880 890 900
APGFAELPEQ LRAQIAEELN VVQLDPLSVV GGDLVDYSVK PNFRALGKRF
910 920 930 940 950
GKTTPRVAQA IREADAKTLV ERLRADNAAT VDVDGEQVVL SADEVVVTEQ
960 970 980 990 1000
PREGWTVASE AGETVALDLE LTPELRRAGV AREVIRLVQD ARKSSGLNIS
1010 1020 1030 1040 1050
DRIHLWWSAT DEMTAQAMAE HAEAISSEVL AVSFTEGTGD ADAYEVVSEE
1060
FGITLRLRKA
Length:1,060
Mass (Da):118,881
Last modified:September 13, 2005 - v1
Checksum:i3DA876E2028CE987
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000088 Genomic DNA. Translation: AAZ55158.1.
RefSeqiYP_289181.1. NC_007333.1.

Genome annotation databases

EnsemblBacteriaiAAZ55158; AAZ55158; Tfu_1120.
GeneIDi3579322.
KEGGitfu:Tfu_1120.
PATRICi23902736. VBITheFus33945_1168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000088 Genomic DNA. Translation: AAZ55158.1 .
RefSeqi YP_289181.1. NC_007333.1.

3D structure databases

ProteinModelPortali Q47QV9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269800.Tfu_1120.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ55158 ; AAZ55158 ; Tfu_1120 .
GeneIDi 3579322.
KEGGi tfu:Tfu_1120.
PATRICi 23902736. VBITheFus33945_1168.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi PRFKTMK.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TFUS269800:GI42-1138-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YX.

Entry informationi

Entry nameiSYI_THEFY
AccessioniPrimary (citable) accession number: Q47QV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3