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Protein

Proteasome-associated ATPase

Gene

arc

Organism
Thermobifida fusca (strain YX)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATPase which is responsible for recognizing, binding, unfolding and translocation of pupylated proteins into the bacterial 20S proteasome core particle. May be essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase may function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis.UniRule annotation

Pathwayi: proteasomal Pup-dependent pathway

This protein is involved in the pathway proteasomal Pup-dependent pathway, which is part of Protein degradation.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway proteasomal Pup-dependent pathway and in Protein degradation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi272 – 2776ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTFUS269800:GI42-1813-MONOMER.
UniPathwayiUPA00997.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome-associated ATPaseUniRule annotation
Alternative name(s):
AAA ATPase forming ring-shaped complexesUniRule annotation
Short name:
ARCUniRule annotation
Proteasomal ATPaseUniRule annotation
Gene namesi
Name:arcUniRule annotation
Ordered Locus Names:Tfu_1809
OrganismiThermobifida fusca (strain YX)
Taxonomic identifieri269800 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptosporangialesNocardiopsaceaeThermobifida
Proteomesi
  • UP000000434 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584Proteasome-associated ATPasePRO_0000397027Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Assembles into a hexameric ring structure that caps the 20S proteasome core. Strongly interacts with the prokaryotic ubiquitin-like protein Pup through a hydrophobic interface; the interacting region of ARC lies in its N-terminal coiled-coil domain. There is one Pup binding site per ARC hexamer ring. Upon ATP-binding, the C-terminus of ARC interacts with the alpha-rings of the proteasome core, possibly by binding to the intersubunit pockets.UniRule annotation

Protein-protein interaction databases

STRINGi269800.Tfu_1809.

Structurei

3D structure databases

ProteinModelPortaliQ47NX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni583 – 5842Docks into pockets in the proteasome alpha-ringUniRule annotation

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili8 – 9083UniRule annotationAdd
BLAST

Domaini

Consists of three main regions, an N-terminal coiled-coil domain that binds to protein Pup and functions as a docking station, an interdomain involved in ARC hexamerization, and a C-terminal ATPase domain of the AAA type.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105DHM. Bacteria.
COG0464. LUCA.
HOGENOMiHOG000245286.
KOiK13527.
OMAiCVDEFKE.
OrthoDBiEOG6MH5BZ.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02112. ARC_ATPase.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR032501. Prot_ATP_ID_OB.
IPR022482. Proteasome_ATPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF16450. Prot_ATP_ID_OB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03689. pup_AAA. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47NX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRDDERHA ERDRDELVAQ VSYLEKELSV LRRKLADSPR HVRLLEDRLQ
60 70 80 90 100
EAQTALAAAN AKNERLVSAL KEARDQIVAL KEEVDRLSQP PSGFGVFLRA
110 120 130 140 150
RDDGTVEIFT NGRKMRVNVS PSVNVDELRP GQEVMLNEAF NVVEASSYET
160 170 180 190 200
VGEVVMLKEI LEDGERVLVI SNHDEERIVR IAEPLRDEPL RAGDSLLLEP
210 220 230 240 250
RSGYVYERIP KAEVEELILE EVPDISYSDI GGLNGQIEMI RDAVELPYLH
260 270 280 290 300
KELFREHKLR PPKGVLLYGP PGCGKTLIAK AVANSLAKQV AEKTGRDVGK
310 320 330 340 350
SFFLNIKGPE LLNKYVGETE RHIRLVFQRA REKASEGTPV IVFFDEMDSI
360 370 380 390 400
FRTRGSGVSS DVENTIVPQL LSEIDGVEGL ENVIVIGASN REDMIDPAIL
410 420 430 440 450
RPGRLDVKIK IERPDAEAAR DIFSKYITPD LPLHPDDLAE HGGSPTATVN
460 470 480 490 500
AMIQRVVERM YAETEENRFL EVTYANGDKE VLYFKDFNSG AMIQNIVDRA
510 520 530 540 550
KKMAIKDYLE NGSKGLRVSH LLQACVDEFS ENEDLPNTTN PDDWARISGK
560 570 580
KGERIVYIRT LVSGKKGADA GRSIDTVANT GQYL
Length:584
Mass (Da):65,368
Last modified:September 13, 2005 - v1
Checksum:i99A28010095E7E36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA. Translation: AAZ55842.1.
RefSeqiWP_011292233.1. NC_007333.1.

Genome annotation databases

EnsemblBacteriaiAAZ55842; AAZ55842; Tfu_1809.
KEGGitfu:Tfu_1809.
PATRICi23904226. VBITheFus33945_1947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA. Translation: AAZ55842.1.
RefSeqiWP_011292233.1. NC_007333.1.

3D structure databases

ProteinModelPortaliQ47NX7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269800.Tfu_1809.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ55842; AAZ55842; Tfu_1809.
KEGGitfu:Tfu_1809.
PATRICi23904226. VBITheFus33945_1947.

Phylogenomic databases

eggNOGiENOG4105DHM. Bacteria.
COG0464. LUCA.
HOGENOMiHOG000245286.
KOiK13527.
OMAiCVDEFKE.
OrthoDBiEOG6MH5BZ.

Enzyme and pathway databases

UniPathwayiUPA00997.
BioCyciTFUS269800:GI42-1813-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02112. ARC_ATPase.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR032501. Prot_ATP_ID_OB.
IPR022482. Proteasome_ATPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF16450. Prot_ATP_ID_OB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03689. pup_AAA. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YX.

Entry informationi

Entry nameiARC_THEFY
AccessioniPrimary (citable) accession number: Q47NX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: September 13, 2005
Last modified: July 6, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.