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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Thermobifida fusca (strain YX)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451ZincUniRule annotation
Binding sitei60 – 601Cysteinyl adenylateUniRule annotation
Binding sitei225 – 2251Cysteinyl adenylateUniRule annotation
Metal bindingi229 – 2291ZincUniRule annotation
Metal bindingi254 – 2541ZincUniRule annotation
Binding sitei281 – 2811Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTFUS269800:GI42-1836-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:Tfu_1832
OrganismiThermobifida fusca (strain YX)
Taxonomic identifieri269800 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptosporangialesNocardiopsaceaeThermobifida
Proteomesi
  • UP000000434 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligasePRO_0000400490Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269800.Tfu_1832.

Structurei

3D structure databases

ProteinModelPortaliQ47NV4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni45 – 484Cysteinyl adenylate bindingUniRule annotation
Regioni83 – 853Cysteinyl adenylate bindingUniRule annotation
Regioni247 – 2493Cysteinyl adenylate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionUniRule annotationAdd
BLAST
Motifi185 – 1906"ERGGDP" regionUniRule annotation
Motifi287 – 2915"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiILAHHYR.
OrthoDBiEOG6RVFXC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47NV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVMRSWPAPT VVALPDHGGP LSVHDTVTGS RRTITPGATA RMYVCGITPY
60 70 80 90 100
DAAHLGHAFT YLTFDLVNRV WRDAGHTVDY VQNITDIDDP LLERAAATGQ
110 120 130 140 150
DWQELAAREI QVFREDMAAL RILPPRAFLG VVESMDLIAD FVARLRAQGV
160 170 180 190 200
TYEVDGDIYF SAADAPGFGS LSQLDRTAML ELFAERGGDP GRAGKKDPLD
210 220 230 240 250
WLLWRAERPG EPAWDTPLGR GRPGWHVECS AISVHELGMG FDLNGGGDDL
260 270 280 290 300
IFPHHEMGAA EACCATGSRP QARHYLHVAM VGLDGEKMSK SRGNLVFVSQ
310 320 330 340 350
LRKAGTDPMA IRLALLAHHY RTAWEWTDAD LRTAEQRLER WRAAAAVENA
360 370 380 390 400
PDARPLLARV RSALADDLDT ATALAAVDEW ADAALSGSGD DPDAPALMRT
410
TVDTLLGVAL DR
Length:412
Mass (Da):44,866
Last modified:September 13, 2005 - v1
Checksum:i033BDB99AEAE1C25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA. Translation: AAZ55865.1.

Genome annotation databases

EnsemblBacteriaiAAZ55865; AAZ55865; Tfu_1832.
KEGGitfu:Tfu_1832.
PATRICi23904272. VBITheFus33945_1970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA. Translation: AAZ55865.1.

3D structure databases

ProteinModelPortaliQ47NV4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269800.Tfu_1832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ55865; AAZ55865; Tfu_1832.
KEGGitfu:Tfu_1832.
PATRICi23904272. VBITheFus33945_1970.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiILAHHYR.
OrthoDBiEOG6RVFXC.

Enzyme and pathway databases

BioCyciTFUS269800:GI42-1836-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YX.

Entry informationi

Entry nameiMSHC_THEFY
AccessioniPrimary (citable) accession number: Q47NV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: September 13, 2005
Last modified: January 20, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.