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Q47MY0 (SYL_THEFY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Tfu_2156
OrganismThermobifida fusca (strain YX) [Complete proteome] [HAMAP]
Taxonomic identifier269800 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeNocardiopsaceaeThermobifida

Protein attributes

Sequence length830 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 830830Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334834

Regions

Motif53 – 6311"HIGH" region HAMAP-Rule MF_00049
Motif593 – 5975"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5961ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q47MY0 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 1A44B3A2BAE1EACE

FASTA83092,640
        10         20         30         40         50         60 
MTAVSGDQTT SGSYDVRALQ EKWQARWAKE NPFTASEDPA DPRPRRYVLD MFPYPSGDLH 

        70         80         90        100        110        120 
MGHAEAFAIG DVIARYHFAR GENVLHPIGW DSFGLPAENA AIKHNFHPAE WTYTNIETQA 

       130        140        150        160        170        180 
ESFRRYAISL DWSRRLHTSD PEYYRWNQWF FLRFFERGLA YRKDGLVNWC PKDQTVLANE 

       190        200        210        220        230        240 
QVIQGKCERC GTEVERRALN QWYFKITDYA QRLLDDMEQL EGKWPERVLT MQRNWIGRSE 

       250        260        270        280        290        300 
GADVQFTIEG REEPVTVFTT RPDTLYGATF FVVAPDSPLA DELCAPEQRE AFEAYRTQVS 

       310        320        330        340        350        360 
KLSDIERQST EREKTGVFLG RYAINPVNGE RIPVWAADYV LADYGHGAIM AVPAHDQRDL 

       370        380        390        400        410        420 
DFARKFNLPV RVVVDTGEPD PAETGIATPG EGTLINSGPL NGLSKSEAIP RIIEILTERG 

       430        440        450        460        470        480 
TGKAAVNYRL RDWLVSRQRF WGTPIPIIHC PECGEVPVPD EELPVRLPDL RGAALAPKGV 

       490        500        510        520        530        540 
SPLAAATDWV NVSCPRCGGP AKRDTDTMDT FVDSSWYFLR YCSPNLDTAP FDQEKVAKWG 

       550        560        570        580        590        600 
PIDQYVGGVE HAILHLLYSR FFTKVLYDMG MVPFTEPFVR LLNQGQVINQ GKAMSKSLGN 

       610        620        630        640        650        660 
GVDLGQEIDR YGVDAVRLTM VFAGPPEEDI DWADVSPGAS VKFLNRAYRV MAEAAAASAP 

       670        680        690        700        710        720 
GVDPATGDLA LRRMTHQTLA KVTEAVEAQR FNVAIARIME LVTAARRAID SGPGAADPAV 

       730        740        750        760        770        780 
REAAEVIAIT LGLFAPYVAE EGWEMLGHTG SVSVGTWREP DPALLVQESV TCVVQVASKV 

       790        800        810        820        830 
RDKLQVSPDI SAEELERLAL ASEKVQNHIA GREIRRIVVR EPKLVNIVVA 

« Hide

References

[1]"Genome sequence and analysis of the soil cellulolytic actinomycete Thermobifida fusca YX."
Lykidis A., Mavromatis K., Ivanova N., Anderson I., Land M., DiBartolo G., Martinez M., Lapidus A., Lucas S., Copeland A., Richardson P., Wilson D.B., Kyrpides N.
J. Bacteriol. 189:2477-2486(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YX.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000088 Genomic DNA. Translation: AAZ56189.1.
RefSeqYP_290212.1. NC_007333.1.

3D structure databases

ProteinModelPortalQ47MY0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269800.Tfu_2156.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ56189; AAZ56189; Tfu_2156.
GeneID3581446.
KEGGtfu:Tfu_2156.
PATRIC23904974. VBITheFus33945_2313.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMALHMGHAE.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycTFUS269800:GI42-2171-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_THEFY
AccessionPrimary (citable) accession number: Q47MY0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries