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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Thermobifida fusca (strain YX)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei596 – 5961ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTFUS269800:GI42-2171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Tfu_2156
OrganismiThermobifida fusca (strain YX)
Taxonomic identifieri269800 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptosporangialesNocardiopsaceaeThermobifida
ProteomesiUP000000434 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 830830Leucine--tRNA ligasePRO_0000334834Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi269800.Tfu_2156.

Structurei

3D structure databases

ProteinModelPortaliQ47MY0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47MY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAVSGDQTT SGSYDVRALQ EKWQARWAKE NPFTASEDPA DPRPRRYVLD
60 70 80 90 100
MFPYPSGDLH MGHAEAFAIG DVIARYHFAR GENVLHPIGW DSFGLPAENA
110 120 130 140 150
AIKHNFHPAE WTYTNIETQA ESFRRYAISL DWSRRLHTSD PEYYRWNQWF
160 170 180 190 200
FLRFFERGLA YRKDGLVNWC PKDQTVLANE QVIQGKCERC GTEVERRALN
210 220 230 240 250
QWYFKITDYA QRLLDDMEQL EGKWPERVLT MQRNWIGRSE GADVQFTIEG
260 270 280 290 300
REEPVTVFTT RPDTLYGATF FVVAPDSPLA DELCAPEQRE AFEAYRTQVS
310 320 330 340 350
KLSDIERQST EREKTGVFLG RYAINPVNGE RIPVWAADYV LADYGHGAIM
360 370 380 390 400
AVPAHDQRDL DFARKFNLPV RVVVDTGEPD PAETGIATPG EGTLINSGPL
410 420 430 440 450
NGLSKSEAIP RIIEILTERG TGKAAVNYRL RDWLVSRQRF WGTPIPIIHC
460 470 480 490 500
PECGEVPVPD EELPVRLPDL RGAALAPKGV SPLAAATDWV NVSCPRCGGP
510 520 530 540 550
AKRDTDTMDT FVDSSWYFLR YCSPNLDTAP FDQEKVAKWG PIDQYVGGVE
560 570 580 590 600
HAILHLLYSR FFTKVLYDMG MVPFTEPFVR LLNQGQVINQ GKAMSKSLGN
610 620 630 640 650
GVDLGQEIDR YGVDAVRLTM VFAGPPEEDI DWADVSPGAS VKFLNRAYRV
660 670 680 690 700
MAEAAAASAP GVDPATGDLA LRRMTHQTLA KVTEAVEAQR FNVAIARIME
710 720 730 740 750
LVTAARRAID SGPGAADPAV REAAEVIAIT LGLFAPYVAE EGWEMLGHTG
760 770 780 790 800
SVSVGTWREP DPALLVQESV TCVVQVASKV RDKLQVSPDI SAEELERLAL
810 820 830
ASEKVQNHIA GREIRRIVVR EPKLVNIVVA
Length:830
Mass (Da):92,640
Last modified:September 13, 2005 - v1
Checksum:i1A44B3A2BAE1EACE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA. Translation: AAZ56189.1.
RefSeqiWP_011292579.1. NC_007333.1.

Genome annotation databases

EnsemblBacteriaiAAZ56189; AAZ56189; Tfu_2156.
KEGGitfu:Tfu_2156.
PATRICi23904974. VBITheFus33945_2313.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA. Translation: AAZ56189.1.
RefSeqiWP_011292579.1. NC_007333.1.

3D structure databases

ProteinModelPortaliQ47MY0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269800.Tfu_2156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ56189; AAZ56189; Tfu_2156.
KEGGitfu:Tfu_2156.
PATRICi23904974. VBITheFus33945_2313.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciTFUS269800:GI42-2171-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YX.

Entry informationi

Entry nameiSYL_THEFY
AccessioniPrimary (citable) accession number: Q47MY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 13, 2005
Last modified: July 22, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.