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Protein

Glutamate 5-kinase

Gene

proB

Organism
Thermobifida fusca (strain YX)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21ATPUniRule annotation1
Binding sitei61SubstrateUniRule annotation1
Binding sitei148SubstrateUniRule annotation1
Binding sitei160Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 181ATPUniRule annotation2
Nucleotide bindingi222 – 228ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTFUS269800:G1G4Q-2195-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Tfu_2179
OrganismiThermobifida fusca (strain YX)
Taxonomic identifieri269800 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptosporangialesNocardiopsaceaeThermobifida
Proteomesi
  • UP000000434 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002300701 – 388Glutamate 5-kinaseAdd BLAST388

Proteomic databases

PRIDEiQ47MV7

Interactioni

Protein-protein interaction databases

STRINGi269800.Tfu_2179

Structurei

3D structure databases

ProteinModelPortaliQ47MV7
SMRiQ47MV7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini285 – 363PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q47MV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTGVGQTRA EIAQARRVVV KVGSSSLTTP QGGLDHERIR DLTDVLAERR
60 70 80 90 100
KAGTEVVLVS SGAVAAGMAP LGLTQRPRDL ATQQAAASVG QGLLLARYTA
110 120 130 140 150
EFARHSLTAA QILLTADDLM RRAQYRNAQR AMSRLLEIGA VPIVNENDTV
160 170 180 190 200
ATHEIRFGDN DRLAALVAHL LRADVLVLLT DVDALYDGNP SLPTSTRITQ
210 220 230 240 250
VNGPEDLVGV DLGSANKRGV GTGGMITKVE SARIATEAGV ATVLTSAANA
260 270 280 290 300
RAALRGDPVG TFFVPAKHRR PSSRQLWLAH ATAGRGSVFL DPGAVHAVVT
310 320 330 340 350
EKASLLPAGV IKVEGDFNAG DPVDLRDENG VLVARGLVNY DSAEIPDLMG
360 370 380
RSTRWLAREL GAEYSREIVH RDDLVVLRNG STSDREAC
Length:388
Mass (Da):41,121
Last modified:September 13, 2005 - v1
Checksum:iF6E921767E0854E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000088 Genomic DNA Translation: AAZ56212.1
RefSeqiWP_011292602.1, NC_007333.1

Genome annotation databases

EnsemblBacteriaiAAZ56212; AAZ56212; Tfu_2179
KEGGitfu:Tfu_2179

Similar proteinsi

Entry informationi

Entry nameiPROB_THEFY
AccessioniPrimary (citable) accession number: Q47MV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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