Reviewed, UniProtKB/Swiss-Prot Q47MU3 (CLPP1_THEFY)

Names and origin

Protein names

Recommended name:
    ATP-dependent Clp protease proteolytic subunit 1
    EC=3.4.21.92
Alternative name(s):
    Endopeptidase Clp 1

Gene names

Name:	clpP1
Ordered Locus Names:	Tfu_2193

Organism

Thermobifida fusca (strain YX) [Complete proteome] [HAMAP]

Taxonomic identifier

269800 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptosporangineae › Nocardiopsaceae › Thermobifida

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity.
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	ATP-dependent Clp protease proteolytic subunit 1 HAMAP MF_00444		PRO_0000226476
Sites
	Active site	121	1	By similarity
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47MU3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: BFED6C1550C3EFC4	FASTA	222	24,792
	10 20 30 40 50 60 MSEFNFDPYR RYGGGMAPMA PQSRYVLPSY IERTAYGVKE MNPYNKLFEE RIIFVGVQID 70 80 90 100 110 120 DTSANDIIAQ MMTLEHIDSD RDITLYINSP GGSFTSLMAI YDTMQFVRPD IQTVCVGQAA 130 140 150 160 170 180 SAAAVLLAGG TKGKRTALPN SRILIHQPAT EGTHGQASDV EIMANEIMRI RHQLETILAK 190 200 210 220 HTGRSVEEIS RDIERDKILT AEEAKEYGIV DDVLPYRKAS LK

References

[1]

"Genome sequence and analysis of the soil cellulolytic actinomycete Thermobifida fusca YX." Lykidis A., Mavromatis K., Ivanova N., Anderson I., Land M., DiBartolo G., Martinez M., Lapidus A., Lucas S., Copeland A., Richardson P., Wilson D.B., Kyrpides N. J. Bacteriol. 189:2477-2486(2007) [PubMed: 17209016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000088 Genomic DNA. Translation: AAZ56226.1.
RefSeq	YP_290249.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S14.009.
Genome annotation databases
GeneID	3581409.
GenomeReviews	Gene locus Tfu_2193 in contig CP000088_GR.
KEGG	tfu:Tfu_2193.
NMPDR	fig|269800.4.peg.1309.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q47MU3.
OMA	Q47MU3. ANEILRM.
Enzyme and pathway databases
BioCyc	TFUS269800:TFU_2193-MON.
Family and domain databases
HAMAP	MF_00444. [Tree]
InterPro	IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_CS. [Graphical view]
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP1_THEFY

Accession

Primary (citable) accession number: Q47MU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)