Q47MS7 (ILVD_THEFY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Tfu_2209

Organism

Thermobifida fusca (strain YX) [Complete proteome] [HAMAP]

Taxonomic identifier

269800 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptosporangineae › Nocardiopsaceae › Thermobifida

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 625	625	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225432
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	199	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q47MS7 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 70685FC7EDE06ACE	FASTA	625	65,802
	10 20 30 40 50 60 MPALRSRTVT HGRNMAGARA LLRATGVARE DFGKPIVAVA NSYTQFVPGH VHLREVADVV 70 80 90 100 110 120 ADAVRAAGGV PREFNTIAVD DGIAMGHGGM LYSLPSRELI ADAVEYMVNA HCADALVCVS 130 140 150 160 170 180 NCDKITPGML LAALRLNIPT VFVSGGPMEA GKVTVVNGTA TTVRKLDLIN PMIASADENV 190 200 210 220 230 240 SEQELNALEE AACPTCGSCS GMFTANSMNC LTEAIGLALP GNGTVLATHT ARKKLYEEAG 250 260 270 280 290 300 RVVVEAARRY YEKDDESVLP LSIATPAAFA NAMALDVAMG GSTNTILHLL AAATEAGVDF 310 320 330 340 350 360 GLDDIDALSR RVPCLCKVAP NSDKYHIEDV HRAGGIPAIM GELARAGLVD TSLPMVHGMT 370 380 390 400 410 420 VGEYVDAWDI ASPNVRPEAV ELFHAAPGGQ RSTEAFSQST RWDSLDLDRE SGCIRSVKHA 430 440 450 460 470 480 YTKDGGLAVL KGNLAPDGAV VKTAGVDEEL WTFSGPAKVF ESQEEAVDGI LNKAVQPGDV 490 500 510 520 530 540 VVIRYEGPKG GPGMQEMLYP TSFLKGRGLG KVCALITDGR FSGGTSGLSI GHASPEAAAG 550 560 570 580 590 600 GDIALVEDGD IITIDIPNRS ITLEVPEDEL NARRERLLKE LGGFRPRNRQ RPVSAALRAY 610 620 AAMATSASTG AARDVSQVER THLHS

References

[1]

"Genome sequence and analysis of the soil cellulolytic actinomycete Thermobifida fusca YX." Lykidis A., Mavromatis K., Ivanova N., Anderson I., Land M., DiBartolo G., Martinez M., Lapidus A., Lucas S., Copeland A., Richardson P., Wilson D.B., Kyrpides N. J. Bacteriol. 189:2477-2486(2007) [PubMed: 17209016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000088 Genomic DNA. Translation: AAZ56242.1.
RefSeq	YP_290265.1. NC_007333.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q47MS7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3581122.
GenomeReviews	Gene locus Tfu_2209 in contig CP000088_GR.
KEGG	tfu:Tfu_2209.
NMPDR	fig|269800.4.peg.1325.
PATRIC	23905098. VBITheFus33945_2371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	Q47MS7.
ProtClustDB	CLSK2769530.
Enzyme and pathway databases
BioCyc	TFUS269800:TFU_2209-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_THEFY

Accession

Primary (citable) accession number: Q47MS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program