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Protein

Glutathione synthetase

Gene

gshB

Organism
Dechloromonas aromatica (strain RCB)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 magnesium or manganese ion per subunit.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi304Magnesium or manganeseUniRule annotation1
Metal bindingi306Magnesium or manganeseUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Glutathione biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:Daro_0010Imported
OrganismiDechloromonas aromatica (strain RCB)Imported
Taxonomic identifieri159087 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeDechloromonas
Proteomesi
  • UP000000550 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Interactioni

Protein-protein interaction databases

STRINGi159087.Daro_0010.

Structurei

3D structure databases

ProteinModelPortaliQ47K62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 333ATP-graspInterPro annotationAdd BLAST186

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D7Z. Bacteria.
COG0189. LUCA.
HOGENOMiHOG000265022.
KOiK01920.
OMAiGMGIFRV.
OrthoDBiPOG091H04J6.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47K62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQQLHFEKH LLGGSSQSLK LAFVLDPLDH LKAWKDSSVA MMRAAEKHGH
60 70 80 90 100
EVFAIDAATL GWRKPDDAHA GGISGEALHL HLRPDDHDWY RETGREWMPL
110 120 130 140 150
TAFDAVIMRK DPPFDFEYLT ATWLLERAEA DGVRVFNRPR ALRDHSEKLA
160 170 180 190 200
IMEFAHFAPA TAISRDMAQI HHFIDEQRDV ILKPLDGMGG SQIFRVHRND
210 220 230 240 250
PNRNVIVETL THEGARTIMA QRYVPEISQG DKRILLIAGK PVPWCLARIP
260 270 280 290 300
KAGETRGNLA VGGTGVAQEL SARDLEIAET LGPILFKRGL MLVGIDVIGN
310 320 330
FLTEINVTSP TCMVEIRQQS GFDAAGAFIT AIEHACGLS
Length:339
Mass (Da):37,643
Last modified:September 13, 2005 - v1
Checksum:i9452134D7DE364B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ44769.1.
RefSeqiWP_011285779.1. NC_007298.1.

Genome annotation databases

EnsemblBacteriaiAAZ44769; AAZ44769; Daro_0010.
KEGGidar:Daro_0010.
PATRICi21598588. VBIDecAro89105_0010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ44769.1.
RefSeqiWP_011285779.1. NC_007298.1.

3D structure databases

ProteinModelPortaliQ47K62.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi159087.Daro_0010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ44769; AAZ44769; Daro_0010.
KEGGidar:Daro_0010.
PATRICi21598588. VBIDecAro89105_0010.

Phylogenomic databases

eggNOGiENOG4105D7Z. Bacteria.
COG0189. LUCA.
HOGENOMiHOG000265022.
KOiK01920.
OMAiGMGIFRV.
OrthoDBiPOG091H04J6.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ47K62_DECAR
AccessioniPrimary (citable) accession number: Q47K62
Entry historyi
Integrated into UniProtKB/TrEMBL: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.