Q47JN4 (METK_DECAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine synthase
Short name=AdoMet synthase
EC=2.5.1.6

Alternative name(s):
MAT
Methionine adenosyltransferase

Gene names

Name:	metK
Ordered Locus Names:	Daro_0188

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas › Dechloromonas aromatica

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity. HAMAP-Rule MF_00086
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. HAMAP-Rule MF_00086
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. HAMAP-Rule MF_00086
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AdoMet synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP methionine adenosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	S-adenosylmethionine synthase HAMAP-Rule MF_00086		PRO_0000240990
Regions
	Nucleotide binding	262 – 269	8	ATP Potential
Sites
	Metal binding	17	1	Magnesium By similarity
	Metal binding	43	1	Potassium By similarity
	Metal binding	266	1	Potassium By similarity
	Metal binding	274	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47JN4 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 6890A0259F180ACA	FASTA	387	41,911
	10 20 30 40 50 60 MSDYLFTSES VGEGHPDKVA DQISDAILDA ILAQDKHSRV AAETLCNTGL VVLAGEITTN 70 80 90 100 110 120 ANVDYIQVAR DTIKRIGYDN TEYGIDYKGC AVLVAYDKQS PDIAQGVNAA YDDNLGQGAG 130 140 150 160 170 180 DQGLMFGYAC DETPSLMPLP IYLSHRMVER QAMLRKDGRL PWARPDAKSQ VTIRYVDGKP 190 200 210 220 230 240 HSIDTVVLST QHSPDISLED LREATIEQII KPVLPKELIK GDIKYLVNPT GRFVVGGPQG 250 260 270 280 290 300 DCGLTGRKII VDTYGGAAPH GGGAFSGKDP SKVDRSAAYA TRYVAKNIVA AGLASRCLVQ 310 320 330 340 350 360 VSYAIGVAEP TSIMVETYGT GKVSNETLTA LVRKHFDLRP KGIVNMLDLL RPIYQKTAAY 370 380 GHFGRDEPEF TWEATDRANL LKSDAGL

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RCB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000089 Genomic DNA. Translation: AAZ44947.1.
RefSeq	YP_283417.1. NC_007298.1.
3D structure databases
ProteinModelPortal	Q47JN4.
SMR	Q47JN4. Positions 2-387.
ModBase	Search...
Protein-protein interaction databases
STRING	159087.Daro_0188.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ44947; AAZ44947; Daro_0188.
GeneID	3569484.
KEGG	dar:Daro_0188.
PATRIC	21598966. VBIDecAro89105_0190.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0192.
HOGENOM	HOG000245710.
KO	K00789.
OMA	IYYAHRL.
ProtClustDB	PRK05250.
Enzyme and pathway databases
BioCyc	DARO159087:GI5B-267-MONOMER.
UniPathway	UPA00315; UER00080.
Family and domain databases
HAMAP	MF_00086. S-AdoMet_synth1.
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
PANTHER	PTHR11964. PTHR11964. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK_DECAR

Accession

Primary (citable) accession number: Q47JN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program