Q47IN4 (PROA_DECAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Daro_0540

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000230000

Sequences

	Sequence		Length	Mass (Da)
	Q47IN4 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 4EBC995D61B89164	FASTA	418	44,269
	10 20 30 40 50 60 MDIKHYMHTV GRQARAASRR LAGATTAEKN AALLAIAAAI RRESAALVAA NQEDLTAARA 70 80 90 100 110 120 AGLETAMLDR LTLSAKGVES MAEGVEQVAT LADPIGEMTD IKYRPSGIQV GKMRVPLGVI 130 140 150 160 170 180 GIIYEARPNV TADAAALCLK SGNAAILRGG SEAIRSNRAI AALVHEGLKA AGLPAESVQV 190 200 210 220 230 240 IDTTDRAAVG ELITMREFVD VIVPRGGKGL IARLLAESRV PMIQHLDGNC HVYLEAEADP 250 260 270 280 290 300 AKALKIVENA KTQRYGTCNT AESLLVDRSV AAMLLPPIAA MLTAKGVEIR GCAETQAIVP 310 320 330 340 350 360 NAIAATEEDY YTEYLAPIIS VKVVSGIDEA IEHINQYSSH HSEAIITDNH PKAMRFLREV 370 380 390 400 410 DSASVMINAS TRFADGFEYG LGAEIGISTD KIHARGPVGL EGLTSQKWVV LGDGHVRG

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RCB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000089 Genomic DNA. Translation: AAZ45297.1.
RefSeq	YP_283767.1. NC_007298.1.
3D structure databases
ProteinModelPortal	Q47IN4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47IN4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3570053.
GenomeReviews	Gene locus Daro_0540 in contig CP000089_GR.
KEGG	dar:Daro_0540.
NMPDR	fig|159087.4.peg.350.
PATRIC	21599692. VBIDecAro89105_0546.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	YGICGAM.
PhylomeDB	Q47IN4.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	DARO159087:DARO_0540-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_DECAR

Accession

Primary (citable) accession number: Q47IN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program