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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Dechloromonas aromatica (strain RCB)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei632 – 6321ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDARO159087:GI5B-561-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Daro_0544
OrganismiDechloromonas aromatica (strain RCB)
Taxonomic identifieri159087 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeDechloromonas
ProteomesiUP000000550 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 870870Leucine--tRNA ligasePRO_0000334747Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi159087.Daro_0544.

Structurei

3D structure databases

ProteinModelPortaliQ47IN0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi629 – 6335"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47IN0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDKYTPADI ERAAQQHWDK TGAARAVEDA TKPKYYCLSM FPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VLSRFHKMQG YNVLQPMGWD AFGMPAENAA LQNNVPPAGW
110 120 130 140 150
TYSNIDYMRQ QLKSLGFAID WEREFATCTP EYYRWEQWLF TRLYEKGLVY
160 170 180 190 200
KKLGTVNWDP VDHTVLANEQ VIDGRGWRSG ALIEKREIPM YYMKITAYAE
210 220 230 240 250
ELLSELDNLP GWPEQVRLMQ KNWIGKSTGV RFAFPLADNP DEKLWVFTTR
260 270 280 290 300
ADTIMGVTFV AVAAEHPLAT KAAANNPELA AFIEECKKGG VAEADIATME
310 320 330 340 350
KKGMPTGIYV THPLTGQQVE VWVGNYVLMS YGDGAVMAVP AHDERDFAFA
360 370 380 390 400
LKYNLPIKQV VAVDGETAFS HEAWAEWYAD KAKGKLVNSG KYDGLGYEAA
410 420 430 440 450
VDAIAADLAA KNLGDKKVQF RLRDWGISRQ RYWGCPIPII HCKTCGDVPV
460 470 480 490 500
PDDQLPVVLP ENVEITGAGS PLAKMPEFYE CQCPKCGGDA RRETDTMDTF
510 520 530 540 550
FESSWYFLRY ACPDNTTAMV DERVAYWCKG GIDQYIGGIE HAILHLLYSR
560 570 580 590 600
FFTKLMRDVG LIGDLGEPFA NLLTQGMVVA PTFYRELDGG KKQWINPADV
610 620 630 640 650
DVVTDERGRP TGATLKTDGL PVVIGGTEKM SKSKNNGVDP QALIDQYGAD
660 670 680 690 700
TARLFIMFAS PPDQSLEWSD AGVEGAYRFL RRLWKTTYDH LQAGLVAAST
710 720 730 740 750
SNDGLSSAQA DLRRKLHQTM GKVADDYGRR KQFNTAIAAV MELLNAYDKC
760 770 780 790 800
DLKDAAGRAL AQESLESIAL LLFPIVPHIG QALYAQLRPG ADAGNAAFPK
810 820 830 840 850
ADPAALKQDE IELMVQVNGK LRGAIRVSAE ADKATIEATA LANEDAIKFM
860 870
EGKPAKKVIV VPGRLVNIVA
Length:870
Mass (Da):96,514
Last modified:May 20, 2008 - v2
Checksum:i47B018626FEE15DB
GO

Sequence cautioni

The sequence AAZ45301.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ45301.1. Different initiation.
RefSeqiWP_041224677.1. NC_007298.1.

Genome annotation databases

EnsemblBacteriaiAAZ45301; AAZ45301; Daro_0544.
KEGGidar:Daro_0544.
PATRICi21599700. VBIDecAro89105_0550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ45301.1. Different initiation.
RefSeqiWP_041224677.1. NC_007298.1.

3D structure databases

ProteinModelPortaliQ47IN0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi159087.Daro_0544.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ45301; AAZ45301; Daro_0544.
KEGGidar:Daro_0544.
PATRICi21599700. VBIDecAro89105_0550.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciDARO159087:GI5B-561-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation."
    Salinero K.K., Keller K., Feil W.S., Feil H., Trong S., Di Bartolo G., Lapidus A.
    BMC Genomics 10:351-351(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RCB.

Entry informationi

Entry nameiSYL_DECAR
AccessioniPrimary (citable) accession number: Q47IN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: July 22, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.