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Q47IN0 (SYL_DECAR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Daro_0544
OrganismDechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]
Taxonomic identifier159087 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeDechloromonas

Protein attributes

Sequence length870 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAZ45301.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 870870Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334747

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif629 – 6335"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q47IN0 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 47B018626FEE15DB

FASTA87096,514
        10         20         30         40         50         60 
MQDKYTPADI ERAAQQHWDK TGAARAVEDA TKPKYYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VLSRFHKMQG YNVLQPMGWD AFGMPAENAA LQNNVPPAGW TYSNIDYMRQ QLKSLGFAID 

       130        140        150        160        170        180 
WEREFATCTP EYYRWEQWLF TRLYEKGLVY KKLGTVNWDP VDHTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALIEKREIPM YYMKITAYAE ELLSELDNLP GWPEQVRLMQ KNWIGKSTGV RFAFPLADNP 

       250        260        270        280        290        300 
DEKLWVFTTR ADTIMGVTFV AVAAEHPLAT KAAANNPELA AFIEECKKGG VAEADIATME 

       310        320        330        340        350        360 
KKGMPTGIYV THPLTGQQVE VWVGNYVLMS YGDGAVMAVP AHDERDFAFA LKYNLPIKQV 

       370        380        390        400        410        420 
VAVDGETAFS HEAWAEWYAD KAKGKLVNSG KYDGLGYEAA VDAIAADLAA KNLGDKKVQF 

       430        440        450        460        470        480 
RLRDWGISRQ RYWGCPIPII HCKTCGDVPV PDDQLPVVLP ENVEITGAGS PLAKMPEFYE 

       490        500        510        520        530        540 
CQCPKCGGDA RRETDTMDTF FESSWYFLRY ACPDNTTAMV DERVAYWCKG GIDQYIGGIE 

       550        560        570        580        590        600 
HAILHLLYSR FFTKLMRDVG LIGDLGEPFA NLLTQGMVVA PTFYRELDGG KKQWINPADV 

       610        620        630        640        650        660 
DVVTDERGRP TGATLKTDGL PVVIGGTEKM SKSKNNGVDP QALIDQYGAD TARLFIMFAS 

       670        680        690        700        710        720 
PPDQSLEWSD AGVEGAYRFL RRLWKTTYDH LQAGLVAAST SNDGLSSAQA DLRRKLHQTM 

       730        740        750        760        770        780 
GKVADDYGRR KQFNTAIAAV MELLNAYDKC DLKDAAGRAL AQESLESIAL LLFPIVPHIG 

       790        800        810        820        830        840 
QALYAQLRPG ADAGNAAFPK ADPAALKQDE IELMVQVNGK LRGAIRVSAE ADKATIEATA 

       850        860        870 
LANEDAIKFM EGKPAKKVIV VPGRLVNIVA 

« Hide

References

[1]"Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation."
Salinero K.K., Keller K., Feil W.S., Feil H., Trong S., Di Bartolo G., Lapidus A.
BMC Genomics 10:351-351(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RCB.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000089 Genomic DNA. Translation: AAZ45301.1. Different initiation.
RefSeqYP_283771.1. NC_007298.1.

3D structure databases

ProteinModelPortalQ47IN0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING159087.Daro_0544.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ45301; AAZ45301; Daro_0544.
GeneID3570057.
KEGGdar:Daro_0544.
PATRIC21599700. VBIDecAro89105_0550.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycDARO159087:GI5B-561-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_DECAR
AccessionPrimary (citable) accession number: Q47IN0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries