Methionine--tRNA ligase - Dechloromonas aromatica (strain RCB)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	Daro_0574

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas ›

Protein attributes

Sequence length	696 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00098
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00098
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 696

696

Methionine--tRNA ligase HAMAP-Rule MF_00098

PRO_0000331810

Regions

Domain

591 – 696

106

tRNA-binding

Motif

12 – 22

11

"HIGH" region HAMAP-Rule MF_00098

Motif

336 – 340

5

"KMSKS" region HAMAP-Rule MF_00098

Sites

Metal binding

143

1

Zinc By similarity

Metal binding

146

1

Zinc By similarity

Metal binding

156

1

Zinc By similarity

Metal binding

159

1

Zinc By similarity

Binding site

339

1

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q47IK0 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: ACC285A967D534AA
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FASTA

696

77,848

        10         20         30         40         50         60 
MSRKILVTSA LPYANGAIHL GHLVEYIQTD IWVRFQKMSG NECWYVCADD THGTPIMLRA 

        70         80         90        100        110        120 
EKEGITPEQL IARVHGEHSR DFAGFHVGFD NYYSTHSDET RDCANDIYLK LRAAGLIETR 

       130        140        150        160        170        180 
TIEQYYDPVK QLFLPDRFIK GECPKCGAKD QYGDNCESCG AAYAPTDLKE PYSAISGAKP 

       190        200        210        220        230        240 
ELRTSEHYFF KLSDPRCEAF LRQYTSRDNG VLQNEAANKM QEWLGAPGEN KLTDWDISRD 

       250        260        270        280        290        300 
APYFGFEIPD APGKYFYVWL DAPIGYMGSF KNLCARNGLD FNEYFKPDAK TELYHFIGKD 

       310        320        330        340        350        360 
ILYFHALFWP AELAHAGFRT PTKIFAHGFL TVDGAKMSKS RGTFITAESF LKTGLNPEWL 

       370        380        390        400        410        420 
RYYYAAKLSA TMEDIDLSLD DFVARVNSDL VGKYVNIASR SAGFITKRFN GQLAPATAEL 

       430        440        450        460        470        480 
PAIKGIQEAA SRIAELYEAR EFGKAMREIM ALTDAANQYV DSVKPWELAK QEGKEVELHA 

       490        500        510        520        530        540 
ACTNALNLFR LLTVLLKPIL PIVAEKVEKF LNIAPLHWAD TQSLLAGGHA INAYEHLMTR 

       550        560        570        580        590        600 
VDPKLIEKLV EANKESLAPA PEAQSQQRHA EHQQNEVTAE SPWEPFCNID DFMKVDLRIV 

       610        620        630        640        650        660 
RIANAEHVEG ADKLVRLTLD VGNNETRNVF AGIKAAYDPA QLIGRLTVMV ANLAPRKMKF 

       670        680        690 
GLSEGMVLAA SDGEGKTPGI FLLSPDSGAQ PGMRVK

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References

[1]

"Complete sequence of Dechloromonas aromatica RCB."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RCB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000089 Genomic DNA. Translation: AAZ45331.1.
RefSeq	YP_283801.1. NC_007298.1.
3D structure databases
ProteinModelPortal	Q47IK0.
SMR	Q47IK0. Positions 2-555.
ModBase	Search...
Protein-protein interaction databases
STRING	159087.Daro_0574.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ45331; AAZ45331; Daro_0574.
GeneID	3568800.
KEGG	dar:Daro_0574.
PATRIC	21599762. VBIDecAro89105_0581.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200400.
KO	K01874.
OMA	RMHGHEV.
ProtClustDB	PRK00133.
Enzyme and pathway databases
BioCyc	DARO159087:GI5B-591-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00098. Met_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR023458. Met-tRNA_ligase_1. IPR014758. Met-tRNA_synth. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_DECAR

Accession

Primary (citable) accession number: Q47IK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 13, 2005
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents