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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Dechloromonas aromatica (strain RCB)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathway: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei302 – 3021NucleophileUniRule annotation
Active sitei355 – 3551Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciDARO159087:GI5B-603-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Daro_0586
OrganismiDechloromonas aromatica (strain RCB)
Taxonomic identifieri159087 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeDechloromonas
ProteomesiUP000000550 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6216211,4-alpha-glucan branching enzyme GlgBPRO_0000260649Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi159087.Daro_0586.

Structurei

3D structure databases

ProteinModelPortaliQ47II8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiQWIDAND.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47II8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHADALYLF NEGRNNQAYR LLGAHADISG TTFRVWAPNA SRVSVVGDFN
60 70 80 90 100
GWHGDVHQLH PLGESGVWEI AVAEAHTGNL YRFEITNRHT GDKLIKSDPY
110 120 130 140 150
GRGFELRPGS AAYVVPPSMH VWGDADWLQQ RAGWDWQQAP VNIYEVHPGS
160 170 180 190 200
WMRHPDGKPY LWGELAERLI PYALAQGYTH LELLPITEHP LDESWGYQTT
210 220 230 240 250
GYFAPTSRYG SADELRAFVD ACHQAGLGVL LDWVPGHFPQ DDWALARFDG
260 270 280 290 300
TALYEHEDPR LGLHADWGTH IFNYGRHEVR SFLMSSAHWW LSEFHFDGLR
310 320 330 340 350
VDAVASMLYL DYSRKHGEWL PNKFGGRENL EAIDFLKQLN AMVHGDFPGA
360 370 380 390 400
LTIAEESTAW PMVSRPTYVG GLGFSMKWNM GWMNDSLRYF HRDPIYRRWH
410 420 430 440 450
HSELTFGQIY AYSENYVLPF SHDEVVHGKG SLLGKMPGDT WQRYANLRLL
460 470 480 490 500
LAWQVLTPGK KLMFMGCEFG QQGEWSEGRE LDWGSLANPE NAAAQRLSAD
510 520 530 540 550
LNRLYRDLPA LHTQDFSALG FEWIDCNDSE HSVLSWLRWG KDGSFVVVVF
560 570 580 590 600
NFTPVPQPAY RLGVPEAGTY VELLNTDSAF YGGSNLGNGG ALTATKGEWM
610 620
GRPANLEVTI PPLGAVVLRR E
Length:621
Mass (Da):70,296
Last modified:September 13, 2005 - v1
Checksum:i9193AE04ECB4B53D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ45343.1.
RefSeqiWP_011286352.1. NC_007298.1.
YP_283813.1. NC_007298.1.

Genome annotation databases

EnsemblBacteriaiAAZ45343; AAZ45343; Daro_0586.
KEGGidar:Daro_0586.
PATRICi21599788. VBIDecAro89105_0594.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ45343.1.
RefSeqiWP_011286352.1. NC_007298.1.
YP_283813.1. NC_007298.1.

3D structure databases

ProteinModelPortaliQ47II8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi159087.Daro_0586.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ45343; AAZ45343; Daro_0586.
KEGGidar:Daro_0586.
PATRICi21599788. VBIDecAro89105_0594.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiQWIDAND.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciDARO159087:GI5B-603-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation."
    Salinero K.K., Keller K., Feil W.S., Feil H., Trong S., Di Bartolo G., Lapidus A.
    BMC Genomics 10:351-351(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RCB.

Entry informationi

Entry nameiGLGB_DECAR
AccessioniPrimary (citable) accession number: Q47II8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: September 13, 2005
Last modified: May 27, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.