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Protein

Biotin synthase

Gene

bioB

Organism
Dechloromonas aromatica (strain RCB)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi71Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi74Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi111Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi142Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi202Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi274Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Daro_0618
OrganismiDechloromonas aromatica (strain RCB)
Taxonomic identifieri159087 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesAzonexaceaeDechloromonas
Proteomesi
  • UP000000550 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003813421 – 327Biotin synthaseAdd BLAST327

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi159087.Daro_0618.

Structurei

3D structure databases

ProteinModelPortaliQ47IF6.
SMRiQ47IF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF. Bacteria.
COG0502. LUCA.
HOGENOMiHOG000239957.
KOiK01012.
OMAiADRFCMG.
OrthoDBiPOG091H01DF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
PANTHERiPTHR22976. PTHR22976. 1 hit.
PTHR22976:SF32. PTHR22976:SF32. 1 hit.
PfamiView protein in Pfam
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SFLDiSFLDF00272. biotin_synthase. 1 hit.
SFLDG01278. biotin_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47IF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQASSLAAVS SASPSAPARR WTVEEVLGLY DMPLMDLIWR AQGVHRENFD
60 70 80 90 100
PNAIQRSTLL SVKTGGCSED CSYCSQSARY DTDTERERLM PLDQVVAAAK
110 120 130 140 150
AAKDKGASRF CMGAAWKGPK DNDLDRVLDM VREVKALGMQ TCVTLGMLKD
160 170 180 190 200
GQAEKLKDAG LDYYNHNLDT DKEFYGQVIK SHTHDDRLDT LDQVRDAGIN
210 220 230 240 250
VCSGGIIGMG ESRKNRAALI VQLANLPKPP ESVPINNLVP IPGTPLADNP
260 270 280 290 300
RLDPFEFVRT IAAARIAMPT SWVRLSAGRQ EMSDELQAMC FLAGANSMFY
310 320
GDFLLTTGNP DIERDDALFA RLGVKAI
Length:327
Mass (Da):35,802
Last modified:September 13, 2005 - v1
Checksum:iF938A05AB3C9EBAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ45375.1.
RefSeqiWP_011286384.1. NC_007298.1.

Genome annotation databases

EnsemblBacteriaiAAZ45375; AAZ45375; Daro_0618.
KEGGidar:Daro_0618.

Similar proteinsi

Entry informationi

Entry nameiBIOB_DECAR
AccessioniPrimary (citable) accession number: Q47IF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 13, 2005
Last modified: August 30, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families