ID   MHPB1_DECAR             Reviewed;         312 AA.
AC   Q47HM4;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 1;
DE            EC=1.13.11.16;
GN   Name=mhpB1; OrderedLocusNames=Daro_0901;
OS   Dechloromonas aromatica (strain RCB).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Dechloromonas.
OX   NCBI_TaxID=159087;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K.,
RA   Schmutz J., Larimer F., Land M., Ivanova N., Richardson P.;
RT   "Complete sequence of Dechloromonas aromatica RCB.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the non-heme iron(II)-dependent oxidative
CC       cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-
CC       dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-
CC       hydroxy-6-ketononatrienedioate, respectively (By similarity).
CC   -!- CATALYTIC ACTIVITY: 3-(2,3-dihydroxyphenyl)propanoate + O(2) = 2-
CC       hydroxy-6-oxonona-2,4-diene-1,9-dioate.
CC   -!- CATALYTIC ACTIVITY: 2,3-dihydroxicinnamic acid + O(2) = 2-hydroxy-
CC       6-oxonona-2,4,7-triene-1,9-dioate.
CC   -!- COFACTOR: Fe(2+) ion (By similarity).
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid
CC       degradation.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the ligB/mhpB extradiol dioxygenase family.
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DR   EMBL; CP000089; AAZ45657.1; -; Genomic_DNA.
DR   RefSeq; YP_284127.1; -.
DR   GeneID; 3570003; -.
DR   GenomeReviews; CP000089_GR; Daro_0901.
DR   KEGG; dar:Daro_0901; -.
DR   NMPDR; fig|159087.4.peg.1255; -.
DR   HOGENOM; Q47HM4; -.
DR   OMA; Q47HM4; PLNPEWD.
DR   BioCyc; DARO159087:DARO_0901-MON; -.
DR   GO; GO:0008669; F:2,3-dihydroxy-phenylpropionate 1,2-dioxygen...; IEA:HAMAP.
DR   GO; GO:0047070; F:3-carboxyethylcatechol 2,3-dioxygenase acti...; IEA:EC.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01653; -; 1.
DR   InterPro; IPR004183; Xdiol_dOase_3B.
DR   Pfam; PF02900; LigB; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Complete proteome; Dioxygenase;
KW   Iron; Oxidoreductase.
FT   CHAIN         1    312       2,3-dihydroxyphenylpropionate/2,3-
FT                                dihydroxicinnamic acid 1,2-dioxygenase 1.
FT                                /FTId=PRO_0000337648.
FT   ACT_SITE    115    115       Proton donor (By similarity).
FT   ACT_SITE    179    179       Proton acceptor (By similarity).
SQ   SEQUENCE   312 AA;  34020 MW;  4E2C6EB37FEED805 CRC64;
     MTIQLKCLSH TPLRGINDPG AATVAEVDAV LAKAKAEVEA FDPELIVVFA PDHYNGLFYD
     LMPPFVIATA ADSVGDYQTM TGPLSVDKDL ALEMAKFILD SDIDIAISHR LQVDHGCTQT
     LEEMTGSLTR YPVIPIIINS VAPPFGPYRR VRKLGEAVGK FIAKLNKRVL ILGTGGLSHE
     PPVPLLSGAN EDIANFLIAG RNPTPEFRAA RQARTLATGM IFGKPECELT PLNPEWDQNF
     MDLLIRGQLD KVDAFDIEEI SKAAGRSTHE VRTWVAAFAA MAASGPYNAH QDYYRPINEW
     IAGYGVVSAS QK
//