Reviewed, UniProtKB/Swiss-Prot Q47HM4 (MHPB1_DECAR)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 1
    EC=1.13.11.16

Gene names

Name:	mhpB1
Ordered Locus Names:	Daro_0901

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity.
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 1 HAMAP MF_01653		PRO_0000337648
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47HM4-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 4E2C6EB37FEED805	FASTA	312	34,020
	10 20 30 40 50 60 MTIQLKCLSH TPLRGINDPG AATVAEVDAV LAKAKAEVEA FDPELIVVFA PDHYNGLFYD 70 80 90 100 110 120 LMPPFVIATA ADSVGDYQTM TGPLSVDKDL ALEMAKFILD SDIDIAISHR LQVDHGCTQT 130 140 150 160 170 180 LEEMTGSLTR YPVIPIIINS VAPPFGPYRR VRKLGEAVGK FIAKLNKRVL ILGTGGLSHE 190 200 210 220 230 240 PPVPLLSGAN EDIANFLIAG RNPTPEFRAA RQARTLATGM IFGKPECELT PLNPEWDQNF 250 260 270 280 290 300 MDLLIRGQLD KVDAFDIEEI SKAAGRSTHE VRTWVAAFAA MAASGPYNAH QDYYRPINEW 310 IAGYGVVSAS QK

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000089 Genomic DNA. Translation: AAZ45657.1.
RefSeq	YP_284127.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3570003.
GenomeReviews	Gene locus Daro_0901 in contig CP000089_GR.
KEGG	dar:Daro_0901.
NMPDR	fig|159087.4.peg.1255.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q47HM4.
OMA	Q47HM4. PLNPEWD.
Enzyme and pathway databases
BioCyc	DARO159087:DARO_0901-MON.
Family and domain databases
HAMAP	MF_01653. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB1_DECAR

Accession

Primary (citable) accession number: Q47HM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)