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Q47HM4 (MHPB1_DECAR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 1
EC=1.13.11.16
Alternative name(s):
3-carboxyethylcatechol 2,3-dioxygenase 1
Gene names
Name:mhpB1
Ordered Locus Names:Daro_0901
OrganismDechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]
Taxonomic identifier159087 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas ›

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP-Rule MF_01653

Catalytic activity

3-(2,3-dihydroxyphenyl)propanoate + O2 = (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP-Rule MF_01653

(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = (2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP-Rule MF_01653

Cofactor

Fe2+ ion By similarity.

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP-Rule MF_01653

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the LigB/MhpB extradiol dioxygenase family.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandIron
   Molecular functionDioxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_process3-phenylpropionate catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function3-carboxyethylcatechol 2,3-dioxygenase activity

Inferred from electronic annotation. Source: HAMAP

ferrous iron binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3123122,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 1 HAMAP-Rule MF_01653
PRO_0000337648

Sites

Active site1151Proton donor By similarity
Active site1791Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q47HM4 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 4E2C6EB37FEED805

FASTA31234,020
        10         20         30         40         50         60 
MTIQLKCLSH TPLRGINDPG AATVAEVDAV LAKAKAEVEA FDPELIVVFA PDHYNGLFYD 

        70         80         90        100        110        120 
LMPPFVIATA ADSVGDYQTM TGPLSVDKDL ALEMAKFILD SDIDIAISHR LQVDHGCTQT 

       130        140        150        160        170        180 
LEEMTGSLTR YPVIPIIINS VAPPFGPYRR VRKLGEAVGK FIAKLNKRVL ILGTGGLSHE 

       190        200        210        220        230        240 
PPVPLLSGAN EDIANFLIAG RNPTPEFRAA RQARTLATGM IFGKPECELT PLNPEWDQNF 

       250        260        270        280        290        300 
MDLLIRGQLD KVDAFDIEEI SKAAGRSTHE VRTWVAAFAA MAASGPYNAH QDYYRPINEW 

       310 
IAGYGVVSAS QK

« Hide

References

[1]"Complete sequence of Dechloromonas aromatica RCB."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RCB.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000089 Genomic DNA. Translation: AAZ45657.1.
RefSeqYP_284127.1. NC_007298.1.

3D structure databases

ProteinModelPortalQ47HM4.
ModBaseSearch...

Protein-protein interaction databases

STRING159087.Daro_0901.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ45657; AAZ45657; Daro_0901.
GeneID3570003.
KEGGdar:Daro_0901.
PATRIC21600430. VBIDecAro89105_0899.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG08474.
HOGENOMHOG000069851.
KOK05713.
OMAAHEIRTW.
ProtClustDBPRK13370.

Enzyme and pathway databases

BioCycDARO159087:GI5B-980-MONOMER.
UniPathwayUPA00714.

Family and domain databases

Gene3D3.40.830.10. 2 hits.
HAMAPMF_01653. MhpB.
InterProIPR023789. DHPP/DHXA_dioxygenase.
IPR004183. Xdiol_dOase_suB.
[Graphical view]
PfamPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMSSF53213. Xdiol_dOase_3B. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMHPB1_DECAR
AccessionPrimary (citable) accession number: Q47HM4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 13, 2005
Last modified: May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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