Q47GX7 (CHEB2_DECAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 2
EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	Daro_1148

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Chemotaxis response regulator protein-glutamate methylesterase 2 HAMAP MF_00099		PRO_0000225456
Regions
	Domain	6 – 123	118	Response regulatory
	Domain	167 – 359	193	CheB-type methylesterase
Sites
	Active site	179	1	By similarity
	Active site	205	1	By similarity
	Active site	301	1	By similarity
Amino acid modifications
	Modified residue	57	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47GX7 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 03A8EE95DB3EDF24	FASTA	359	37,941
	10 20 30 40 50 60 MADKIKVMIV DDSALVRQVV AQALASDPGI EVTDVASDPI FALQKMKVRW PDVLVIDIEM 70 80 90 100 110 120 PRMDGITFLK KIMAEHPTPT VICSSLAEKG AQATFEALAA GAVSIITKPK IGLKSFLEDA 130 140 150 160 170 180 SNDIVQAVRS AARANLRALS ASANPAINRP KLSADAMLST GSHRALERTT DQLVAIGTST 190 200 210 220 230 240 GGTQALEAVL TRLPATAPGI VIVQHMPEKF TAMFAERLDG LCQIEVREAR NGDRVLPGLA 250 260 270 280 290 300 LIAPGGRHMM LKRNGAQYVV DVADGPLINR HKPSVDVLFR SVAKFAGANA LGIIMTGMGD 310 320 330 340 350 DGARGMKEMH DAGAKTIAQD ESSCVVFGMP KEAIKLGGVD QTMPLQQIPG AIVGYGKSC

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RCB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000089 Genomic DNA. Translation: AAZ45904.1.
RefSeq	YP_284374.1. NC_007298.1.
3D structure databases
ProteinModelPortal	Q47GX7.
SMR	Q47GX7. Positions 169-348.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47GX7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3570108.
GenomeReviews	Gene locus Daro_1148 in contig CP000089_GR.
KEGG	dar:Daro_1148.
NMPDR	fig|159087.4.peg.734.
PATRIC	21600948. VBIDecAro89105_1149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	PGGKHML.
PhylomeDB	Q47GX7.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	DARO159087:DARO_1148-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_DECAR

Accession

Primary (citable) accession number: Q47GX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program