ID   MHPB2_DECAR             Reviewed;         317 AA.
AC   Q47GC9;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 2;
DE            EC=1.13.11.16;
GN   Name=mhpB2; OrderedLocusNames=Daro_1353;
OS   Dechloromonas aromatica (strain RCB).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Dechloromonas.
OX   NCBI_TaxID=159087;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K.,
RA   Schmutz J., Larimer F., Land M., Ivanova N., Richardson P.;
RT   "Complete sequence of Dechloromonas aromatica RCB.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the non-heme iron(II)-dependent oxidative
CC       cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-
CC       dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-
CC       hydroxy-6-ketononatrienedioate, respectively (By similarity).
CC   -!- CATALYTIC ACTIVITY: 3-(2,3-dihydroxyphenyl)propanoate + O(2) = 2-
CC       hydroxy-6-oxonona-2,4-diene-1,9-dioate.
CC   -!- CATALYTIC ACTIVITY: 2,3-dihydroxicinnamic acid + O(2) = 2-hydroxy-
CC       6-oxonona-2,4,7-triene-1,9-dioate.
CC   -!- COFACTOR: Fe(2+) ion (By similarity).
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid
CC       degradation.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the ligB/mhpB extradiol dioxygenase family.
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DR   EMBL; CP000089; AAZ46102.1; -; Genomic_DNA.
DR   RefSeq; YP_284572.1; -.
DR   GeneID; 3569243; -.
DR   GenomeReviews; CP000089_GR; Daro_1353.
DR   KEGG; dar:Daro_1353; -.
DR   NMPDR; fig|159087.4.peg.1140; -.
DR   HOGENOM; Q47GC9; -.
DR   OMA; Q47GC9; INWIELM.
DR   BioCyc; DARO159087:DARO_1353-MON; -.
DR   GO; GO:0008669; F:2,3-dihydroxy-phenylpropionate 1,2-dioxygen...; IEA:HAMAP.
DR   GO; GO:0047070; F:3-carboxyethylcatechol 2,3-dioxygenase acti...; IEA:EC.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01653; -; 1.
DR   InterPro; IPR004183; Xdiol_dOase_3B.
DR   Pfam; PF02900; LigB; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Complete proteome; Dioxygenase;
KW   Iron; Oxidoreductase.
FT   CHAIN         1    317       2,3-dihydroxyphenylpropionate/2,3-
FT                                dihydroxicinnamic acid 1,2-dioxygenase 2.
FT                                /FTId=PRO_0000337649.
FT   ACT_SITE    115    115       Proton donor (By similarity).
FT   ACT_SITE    179    179       Proton acceptor (By similarity).
SQ   SEQUENCE   317 AA;  34499 MW;  2AE4707EA6D0BE23 CRC64;
     MAALLQCISH SPMKGYVDPS PAIVSDVEVA IARMRKELVD FDPEVIFLFA PDHYNGFFLD
     VMPQFCVGIA ATSVGDYRTT PGPINVPREI ARSCAEHLIS NDIDVAISYR MQVDHGMVQP
     LEELMGGLNA YPVVPLFVNG VAPPLISIRR ARLFGAAVGE YAKKLNKRCL FIGSGGLSHN
     PPVPQIDTAT EEFAEFLIAG RNPSPERRAA RQQRTKDAAI RFAAGDSQLH PINSVWDEAF
     MADLVNQDWG ALDAYRNSEI TEQAGISTHE AKSWVAAHAA MNAATSGGYM AEVRYYKAIP
     EWIVGYGAMA GSAEQAT
//