Q47G55 (URE1_DECAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease subunit alpha
EC=3.5.1.5

Alternative name(s):
Urea amidohydrolase subunit alpha

Gene names

Name:	ureC
Ordered Locus Names:	Daro_1427

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity. HAMAP MF_01953
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01953.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity. HAMAP MF_01953
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro urease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Urease subunit alpha HAMAP MF_01953		PRO_0000234153
Regions
	Domain	132 – 569	438	Urease
Sites
	Active site	323	1	Proton donor By similarity
	Metal binding	137	1	Nickel 2 By similarity
	Metal binding	139	1	Nickel 2 By similarity
	Metal binding	220	1	Nickel 1; via carbamate group By similarity
	Metal binding	220	1	Nickel 2; via carbamate group By similarity
	Metal binding	249	1	Nickel 1 By similarity
	Metal binding	275	1	Nickel 1 By similarity
	Metal binding	363	1	Nickel 2 By similarity
	Binding site	222	1	Substrate By similarity
Amino acid modifications
	Modified residue	220	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47G55 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: B10702E8BE43CABC	FASTA	569	60,409
	10 20 30 40 50 60 MMANKITRQA YAEMFGPTTG DRMRLADTEL IIEVEKDYTI YGEEVKFGGG KVIRDGMGQG 70 80 90 100 110 120 QRLSAETVDT VITNALIVDA VTGIVKADIG LKEGRIAAIG KAGNPDIQPG VTIVIGPGTE 130 140 150 160 170 180 VIAGEGMIVT AGGIDSHIHF ICPQQIDEAL YSGVTTMIGG GTGPATGTFA TTCTPGPWHI 190 200 210 220 230 240 HRMLEAADAF PMNLGFLGKG NASLPEALRE QVEAGVMGLK LHEDWGTTPA AIDCCLTVAD 250 260 270 280 290 300 EMDVQVAIHS DTLNESGFVE ATLGAFKGRT IHTFHTEGAG GGHAPDIIKA AGLPNVLPSS 310 320 330 340 350 360 TNPTMPYTVN TIDEHLDMLM VCHHLDPSIA EDIAFAESRI RRETIAAEDI LHDLGAFSMM 370 380 390 400 410 420 SSDSQAMGRV GEVIIRTWQA AHKMKLQRGA LPEDSARNDN FRVKRYIAKY TINPALTHGI 430 440 450 460 470 480 AHTVGSVEVG KLADLVLWKP AFFGVKPSLI LKGGMIAAAA MGDPNASIPT PQPVHYRPMF 490 500 510 520 530 540 GSFGKALKTS VTFISQAALN NPAIAALGLS KPLVAVSGTR TLTKADMVHN GATPEITVDP 550 560 ETYVVKADGV HLVCEPATEL PLAQRYFLF

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RCB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000089 Genomic DNA. Translation: AAZ46176.1.
RefSeq	YP_284646.1. NC_007298.1.
3D structure databases
ProteinModelPortal	Q47G55.
SMR	Q47G55. Positions 4-569.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47G55.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3569073.
GenomeReviews	Gene locus Daro_1427 in contig CP000089_GR.
KEGG	dar:Daro_1427.
NMPDR	fig|159087.4.peg.1280.
PATRIC	21601488. VBIDecAro89105_1411.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0804.
HOGENOM	HBG357507.
OMA	TIHAFHT.
ProtClustDB	PRK13207.
Enzyme and pathway databases
BioCyc	DARO159087:DARO_1427-MONOMER.
Family and domain databases
HAMAP	MF_01953. Urease_alpha. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011059. Metal-dep_hydrolase_composite. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017950. Urease_asu_CS. [Graphical view]
KO	K01428.
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
SUPFAM	SSF51338. Metalo_hydrolase. 2 hits.
TIGRFAMs	TIGR01792. Urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URE1_DECAR

Accession

Primary (citable) accession number: Q47G55

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program