Reviewed, UniProtKB/Swiss-Prot Q47EP3 (DNAE2_DECAR)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Daro_1942

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas

Protein attributes

Sequence length	1027 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1027	1027	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000070590

Sequences

	Sequence		Length	Mass (Da)
	Q47EP3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: C83D577695B12DCF	FASTA	1,027	113,395
	10 20 30 40 50 60 MSLPEYAELH CLSNFSFLRG ASHPEELAAR ALAQGYAALA LTDECSLAGV VRAHLAAKKH 70 80 90 100 110 120 GLKFIVGSEM MTVDGLKLVF LACNRHGYGN LSALITLARR RAEKGGYTLH RNDLESISPS 130 140 150 160 170 180 GALPDCLVLW VPGNNPSSAD GEWLVSRFPG RSWIAVELHA GPEDAERLAG LQSLGEVCGL 190 200 210 220 230 240 PLVATGDVHM HIKARRPVQD VLTALRLKST VFEAGYALFP NGERHLRSRL RLSRLYPPEL 250 260 270 280 290 300 LAETLNIAAR CEFLLDELRY EYPEEIIPSG ETPASWLRSE TERGLQRRYP AGVPGSVRER 310 320 330 340 350 360 IEHELGLIAE MAYEAYFLTV YDIVCYARSQ DILCQGRGSA ANSAVCYALG VTEVDPARSA 370 380 390 400 410 420 LLFERFVSKE RGEPPDIDVD FEHERREDVI QYIYTKYGRE RAALAAALIT YRTKGALRDA 430 440 450 460 470 480 GRALGFGIAQ INALTASLAW WDKREQLPER FAELGLDPHA PRVEKWLAIA EALRGFPRHL 490 500 510 520 530 540 TQHVGGFVIS RGPLSRLVPV ENAAMSARSV IQWDKDDLDA MGLMKVDILA LGMLSAIRRM 550 560 570 580 590 600 LQIVGETTGR PMKMQDIPAE DPATYEMLCH ADSMGVFQVE SRAQMAMLPR LRPQNFYDLV 610 620 630 640 650 660 VEVALVRPGP IQGDMVHPYL KRRQGRERIE EISPAVDAVL ERTYGVPIFQ EQVMQLAVVA 670 680 690 700 710 720 ANFTPGEADQ LRRAMAAWKR KGGLEPFEQK LLAGMAANDL PESFARRIIA QIQGFGEYGF 730 740 750 760 770 780 PESHAASFAL LVYASAWLKR HHPAAFLCGL LNSQPMGFYS PSMLIQDARR HGVRVLPPDV 790 800 810 820 830 840 MTSDWDSRLD ERGAVRLGLR EISGFSVAAA KRITAVCREN QPFLNVADLA ARAGLQRRDL 850 860 870 880 890 900 DLLAAGDALQ GLAGHRRQAA WAATVAVVQG DLFDGTPVVE AEIELPAPSD GENLVADYRS 910 920 930 940 950 960 LGLTLRSHPL SLLRQYLAER RFVTAADLKM AGHHTLIRSV GIVVGRQRPG TATGIVFVTL 970 980 990 1000 1010 1020 EDETGLSNVV VHPQLVEKQR RELLGSTLLG VYGQLQVEGE VVHLVAKRLV DLSAWLGRLE TVSRDFH

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000089 Genomic DNA. Translation: AAZ46688.1.
RefSeq	YP_285158.1.
3D structure databases
SMR	Q47EP3. Positions 1-864.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47EP3.
Genome annotation databases
GeneID	3567871.
GenomeReviews	Gene locus Daro_1942 in contig CP000089_GR.
KEGG	dar:Daro_1942.
NMPDR	fig|159087.4.peg.1954.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	MGFYSPD.
Enzyme and pathway databases
BioCyc	DARO159087:DARO_1942-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_DECAR

Accession

Primary (citable) accession number: Q47EP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)