Q47C53 (SYK_DECAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase
EC=6.1.1.6

Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS

Gene names

Name:	lysS
Ordered Locus Names:	Daro_2848

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas › Dechloromonas aromatica

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity. HAMAP-Rule MF_00252
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00252
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00252.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Lysine--tRNA ligase HAMAP-Rule MF_00252		PRO_1000012873
Sites
	Metal binding	412	1	Magnesium 1 By similarity
	Metal binding	419	1	Magnesium 1 By similarity
	Metal binding	419	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47C53 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 8F14784765ED0E9C	FASTA	501	56,869
	10 20 30 40 50 60 MSNAEQQAPV QQDENQLIAE RRAKLAEWRK TGKAFPNDFQ RENTAAKVLE GYGEKTAEEL 70 80 90 100 110 120 EGLPIEVKVA GRLMLKRVMG KASFATIQDL SGRIQIYITR DRVGEEVYAD FKTWDLGDII 130 140 150 160 170 180 GVTGSLMKTK TGELSIQAAE IRLLTKSLRP LPDKFHGLAD QEMKYRQRYV DLIMNEETRF 190 200 210 220 230 240 TFRARSAIVA SIRNYMTGHG FMEVETPMMH PIPGGASAKP FVTHHNALDM QQFLRIAPEL 250 260 270 280 290 300 YLKRLVVGGF EKVFEINRNF RNEGLSPRHN PEFTMMEFYE AYANYHTLMD FTEGLLRHAA 310 320 330 340 350 360 REALGKEVFV YQGRELDLSK PFHRLTINQA IQRQHPEFSD AELADPEFLK AKIKHFGEPL 370 380 390 400 410 420 KPGGLGSLQL QLFEACAEAH CWEPTFIIDY PVEVSPLARA SDTNPEITER FELFIVGREI 430 440 450 460 470 480 ANGFSELNDA EDQAARFQEQ MKAKDAGDEE AMYYDADFIR ALEYGLPPTG GCGIGIDRLI 490 500 MLLTDAAAIR DVILFPSMRP E

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RCB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000089 Genomic DNA. Translation: AAZ47578.1.
RefSeq	YP_286048.1. NC_007298.1.
3D structure databases
ProteinModelPortal	Q47C53.
SMR	Q47C53. Positions 15-500.
ModBase	Search...
Protein-protein interaction databases
STRING	159087.Daro_2848.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ47578; AAZ47578; Daro_2848.
GeneID	3566410.
KEGG	dar:Daro_2848.
PATRIC	21604366. VBIDecAro89105_2830.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1190.
HOGENOM	HOG000236578.
KO	K04567.
OMA	EHKLEQP.
ProtClustDB	PRK00484.
Enzyme and pathway databases
BioCyc	DARO159087:GI5B-2919-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_00252. Lys_tRNA_synth_class2.
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR002313. Lys-tRNA-ligase_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. PTHR22594. 1 hit. PTHR22594:SF4. PTHR22594:SF4. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK_DECAR

Accession

Primary (citable) accession number: Q47C53

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	May 29, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program