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Q47BK5

- SYI_DECAR

UniProt

Q47BK5 - SYI_DECAR

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Daro_3046
Organism
Dechloromonas aromatica (strain RCB)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei570 – 5701Aminoacyl-adenylate By similarity
Binding sitei614 – 6141ATP By similarity
Metal bindingi895 – 8951Zinc By similarity
Metal bindingi898 – 8981Zinc By similarity
Metal bindingi915 – 9151Zinc By similarity
Metal bindingi918 – 9181Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDARO159087:GI5B-3119-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Daro_3046
OrganismiDechloromonas aromatica (strain RCB)
Taxonomic identifieri159087 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeDechloromonas
ProteomesiUP000000550: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligaseUniRule annotationPRO_0000098378Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi159087.Daro_3046.

Structurei

3D structure databases

ProteinModelPortaliQ47BK5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi611 – 6155"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47BK5-1 [UniParc]FASTAAdd to Basket

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MADYKDSLNL PDTAFPMRGD LPKREPQWVA QWQEKKLYQR IREICAGRPR    50
FTLHDGPPYA NGDIHIGHAV NKVLKDIIVR SKTLSGFDAP YVPGWDCHGL 100
PIEHQIEKLH GKAIPADKVR ELSRAYAAEQ VERQKKDFIR LGVLGDWGNP 150
YLTMNFSAEA GEIRALGKIL EQGYLYQGLK PVNWCLDCGS ALAEAEVEYE 200
DKNSPAIDVA FEVHENHTAK LAAAFGLTHL RGPAFAVIWT TTPWTLPANE 250
AVSVHPDLTY DLIETEKGAL ILVRELAEAA LKRYGLEGTV AGSCTGDKLD 300
QMLLKHPFQN RDVAIICGTH VTTEAGTGLV HTAPAHGVDD YNIGKKYGLP 350
VNNPVGNDGK FISTTPALSV GELAGKTVWE ANPQVLQELE ARARLLKNER 400
IQHSYPHCWR HKTPIIFRAT TQWFIGMENK KNEDASTLRW IAERAVDETQ 450
FFPAWGRARL EGMMKTRPDW CVSRQRNWGV PIPFFLHKET GQPHPRTAEL 500
IEQVALRVEK SGIEAWFSLD AAELLGAEAD QYVKMKDTLD VWFDSGTTHW 550
HVMRGSHAAD HTYPADLYLE GSDQHRGWFQ SSLLSGCAID GRAPYKGLLT 600
HGFVVDGKGH KMSKSKGNVI APQQVSDKMG ADILRLWTAS TDYSGELTIS 650
DEILKRVVEG YRRIRNTLRF LLANVSDFDA ATDMLPIDQW LEIDRYALAL 700
TRELQDGCRA DFDKYEFHRV VQALQTFCSE DLGGFYLDIL KDRLYTTAPK 750
SVARRSAQSA LWHITQAFVR LLAPITAFTA EEVWQVLTGK ADDSVMFQVW 800
HDLPALAGEG DLLAKWALIR TARADVTKAL EAQREAGKIG SALQAAVEIH 850
CGGEKYEALA SLGDDLKFVF ICSSTVAVRD DNEQVIATPL EHAKCERCWH 900
VREDVGANTE HPTLCGRCVS NLYGEGEVRG CA 932
Length:932
Mass (Da):104,052
Last modified:September 13, 2005 - v1
Checksum:i06C1ECF9BC461869
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000089 Genomic DNA. Translation: AAZ47776.1.
RefSeqiYP_286246.1. NC_007298.1.

Genome annotation databases

EnsemblBacteriaiAAZ47776; AAZ47776; Daro_3046.
GeneIDi3568250.
KEGGidar:Daro_3046.
PATRICi21604760. VBIDecAro89105_3025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000089 Genomic DNA. Translation: AAZ47776.1 .
RefSeqi YP_286246.1. NC_007298.1.

3D structure databases

ProteinModelPortali Q47BK5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 159087.Daro_3046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ47776 ; AAZ47776 ; Daro_3046 .
GeneIDi 3568250.
KEGGi dar:Daro_3046.
PATRICi 21604760. VBIDecAro89105_3025.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DARO159087:GI5B-3119-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation."
    Salinero K.K., Keller K., Feil W.S., Feil H., Trong S., Di Bartolo G., Lapidus A.
    BMC Genomics 10:351-351(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RCB.

Entry informationi

Entry nameiSYI_DECAR
AccessioniPrimary (citable) accession number: Q47BK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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