Reviewed, UniProtKB/Swiss-Prot Q47BG6 (SYV_DECAR)

Names and origin

Protein names

Recommended name:
    Valyl-tRNA synthetase
    EC=6.1.1.9
Alternative name(s):
    Valine--tRNA ligase
      Short name=ValRS

Gene names

Name:	valS
Ordered Locus Names:	Daro_3085

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas

Protein attributes

Sequence length	943 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity.
Catalytic activity	ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP MF_02004
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	valyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP valine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 943	943	Valyl-tRNA synthetase HAMAP MF_02004		PRO_0000224470
Regions
	Coiled coil	875 – 934	60	Potential
	Motif	45 – 55	11	"HIGH" region HAMAP MF_02004
	Motif	541 – 545	5	"KMSKS" region HAMAP MF_02004
Sites
	Binding site	544	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47BG6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 9CB7A2DAC73D3442	FASTA	943	106,500
	10 20 30 40 50 60 MELAKAFEPA DIERRWYPEW ETQNYFAAGV DASKADNFCI LLPPPNVTGT LHMGHGFNQT 70 80 90 100 110 120 IMDALTRYYR MRGHNTLWQP GTDHAGIATQ IVVERQLDAQ GISRHDLGRE KFLEKVWEWK 130 140 150 160 170 180 EYSGNTITKQ MRRMGTSPDW KRERFTMDAG LNKVVTETFV RLFNEGLIYR GKRLVNWDPK 190 200 210 220 230 240 LNTAVSDLEV VQEEEDGFMW HIRYPLADGS DSLVVATTRP ETMLGDTAVM VHPEDERYKH 250 260 270 280 290 300 MIGQMVKLPL TDREIPIIAD SYVDLEFGTG CVKVTPAHDF NDYAVGQRHG LPMISILTLD 310 320 330 340 350 360 AKVNENAPEK YRGLDRFDAR KAVVADLEAL GILEKTDKHK LKVPRGDRTN VVIEPMLTDQ 370 380 390 400 410 420 WFVAMSKPGD DGKSITEKAL DVVHSGEIKF YPENWVNTYN QWLNNIQDWC ISRQLWWGHQ 430 440 450 460 470 480 IPAWYGDNGQ IFVAHSEAEA KAEAAKQGYT GTLKRDEDVL DTWFSSALWP FSTLDWTGDE 490 500 510 520 530 540 AIDAANPLLK QYLPSSVLVT GFDIIFFWVA RMVMMTKQIT GQIPFKHVYV HGLIRDGEGQ 550 560 570 580 590 600 KMSKSKGNVL DPIDLIDGIG LEALIEKRTT GLMNPKQAES IAKKTKKEFP EGIASFGTDA 610 620 630 640 650 660 LRFTFASLAS PGRDIKFDLN RCDGYRNFCN KLWNATRFVL MNVEGHDLAL EHQQNGPACG 670 680 690 700 710 720 GSAPLEFSFA DRWIVSQLQR VEQEVEQHFT DYRFDLIAQA IYKFIWDEFC DWYLEIAKVE 730 740 750 760 770 780 IQTGNDAQQR GARRTLVRTL EAVLRLAHPL IPFITEELWQ TVAPIAGRKT HDSIMLAAYP 790 800 810 820 830 840 RAEEYKIDAA SEAKVERLKA LAYACRNLRG EMNVSPALRM PLLVAGGGAE ISEFAAILQA 850 860 870 880 890 900 LGKLSEVQIV DDMPADAMAP VAVVGETRLM LKVEIDVAAE RIRLAKEIEK LEKQISIAQG 910 920 930 940 KLANEGFVAR APAAVIDQEK QRVADFTATL EQLKPQLAKL GQA

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000089 Genomic DNA. Translation: AAZ47815.1.
RefSeq	YP_286285.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3566514.
GenomeReviews	Gene locus Daro_3085 in contig CP000089_GR.
KEGG	dar:Daro_3085.
NMPDR	fig|159087.4.peg.3070.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q47BG6.
OMA	Q47BG6. WPDETPE.
Enzyme and pathway databases
BioCyc	DARO159087:DARO_3085-MON.
Family and domain databases
HAMAP	MF_02004. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR002303. Val-tRNA_synth_Ia. IPR019754. Val-tRNA_synth_Ia_N. IPR019499. Val-tRNA_synth_Ia_tRNA-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF5. tRNA-synt_val. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF10458. Val_tRNA-synt_C. 1 hit. [Graphical view]
PRINTS	PR00986. TRNASYNTHVAL.
TIGRFAMs	TIGR00422. valS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYV_DECAR

Accession

Primary (citable) accession number: Q47BG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)