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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

Daro_3096

Organism
Dechloromonas aromatica (strain RCB)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101SubstrateBy similarity1
Metal bindingi102Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciDARO159087:GI5B-3141-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:Daro_3096
OrganismiDechloromonas aromatica (strain RCB)
Taxonomic identifieri159087 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeDechloromonas
Proteomesi
  • UP000000550 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000138361 – 163Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolaseAdd BLAST163

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi159087.Daro_3096.

Structurei

3D structure databases

ProteinModelPortaliQ47BF5.
SMRiQ47BF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 82Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiEDNTLVR.
OrthoDBiPOG091H03SM.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47BF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFKTPDLCD EFESELGKTV RVVTPMFQRY GGRTSFSGQI VTLKIFEDNS
60 70 80 90 100
LVREVFGEDG KGKVLVIDGG GSLRCALVGD QLAILANKNG WEGVVVYGCI
110 120 130 140 150
RDSGDINGID IGVRALNTHP QKTLKKGVGD KNVAVTFGGV TFNPGEYLYA
160
DEDGVLVSGK ALT
Length:163
Mass (Da):17,451
Last modified:September 13, 2005 - v1
Checksum:i68F51B34D2E5F261
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ47826.1.
RefSeqiWP_011288824.1. NC_007298.1.

Genome annotation databases

EnsemblBacteriaiAAZ47826; AAZ47826; Daro_3096.
KEGGidar:Daro_3096.
PATRICi21604860. VBIDecAro89105_3075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ47826.1.
RefSeqiWP_011288824.1. NC_007298.1.

3D structure databases

ProteinModelPortaliQ47BF5.
SMRiQ47BF5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi159087.Daro_3096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ47826; AAZ47826; Daro_3096.
KEGGidar:Daro_3096.
PATRICi21604860. VBIDecAro89105_3075.

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiEDNTLVR.
OrthoDBiPOG091H03SM.

Enzyme and pathway databases

BioCyciDARO159087:GI5B-3141-MONOMER.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRRAAH_DECAR
AccessioniPrimary (citable) accession number: Q47BF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.