Q47BD0 (ACEK_DECAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Daro_3121

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288286
Regions
	Nucleotide binding	324 – 330	7	ATP By similarity
Sites
	Active site	380	1	By similarity
	Binding site	345	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47BD0 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 2F8FE52C80B4584A	FASTA	593	69,788
	10 20 30 40 50 60 MKISVGLYGT NAHQIALAMI QGFNKHYTLF RQTSREAKTR FEQADWLGVH KAVKERIRFY 70 80 90 100 110 120 DDRVDECVER LRNQFDAASI DDTTWQQVKL LYIGLLLNHK QPELAETFFN SVTTKILHRN 130 140 150 160 170 180 YFHNDFIFVR PSISTENIEG DDTQTYRSYY AKEDGLRGTV LKIVKDFDWH RPFVDLEHDV 190 200 210 220 230 240 EHVYHAVRHF LNGMPPREVN FQIQVLGSAF YRNKAAYIIG KAINGATEYP FTIPVLQNEA 250 260 270 280 290 300 GQLYLDTILL DAWRIGLLFS LSRAYFMVDM EVPSGYVQFL RSILPAKPRS ELYIMLGLGK 310 320 330 340 350 360 QGKTMFFRDL IYHLHHSEDK FIMAPGIRGL VMLVFTLPSY PYVFKLIKDV FGSSKEMDRA 370 380 390 400 410 420 TVKKKFMMVK QVDRVGRMAD TLEFSNVMFP LKRFNDEVLA ELQQLAPSCF EVDGDQLIIK 430 440 450 460 470 480 HLYIERRMEP LNIHLDRMER ANNVERLEHV IREYGSAIRE MAQANIFPGD MLWKNFGVTR 490 500 510 520 530 540 FGRVVFYDYD EIEYMTDIKF RQIPPAPDFE TEMSGEVWYA VSRNDVFPEE FATFLLTSPQ 550 560 570 580 590 VRKIFIKYHK DLLSPRFWLE AQEKIRSGYV EDFFPYPQEL RFINRQTDLT EEQ

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RCB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000089 Genomic DNA. Translation: AAZ47851.1.
RefSeq	YP_286321.1. NC_007298.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q47BD0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3568172.
GenomeReviews	Gene locus Daro_3121 in contig CP000089_GR.
KEGG	dar:Daro_3121.
NMPDR	fig|159087.4.peg.3260.
PATRIC	21604914. VBIDecAro89105_3102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
PhylomeDB	Q47BD0.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	DARO159087:DARO_3121-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_DECAR

Accession

Primary (citable) accession number: Q47BD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program