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Q47A87 (NAPA_DECAR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Daro_3515
OrganismDechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]
Taxonomic identifier159087 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeDechloromonas

Protein attributes

Sequence length837 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 837806Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045983

Regions

Domain37 – 93574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding441Iron-sulfur (4Fe-4S) By similarity
Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding791Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q47A87 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: E16F8200D0517622

FASTA83793,395
        10         20         30         40         50         60 
MKLNRRDFIK ANAAAAAISA AGLSVPGAAV AQGKDEIRWD KAACRFCGTG CGVLVGTQDG 

        70         80         90        100        110        120 
RVVATQGDPD APVNRGLNCI KGYFLSKIMY GADRLKTPML RMKDGKYDKN GEFTPISWTK 

       130        140        150        160        170        180 
AFDIMEEKAK ATMKAKGPNG LAMFGSGQWT IWEGYAASKL MKAGFRTNNL DPNARHCMAS 

       190        200        210        220        230        240 
AVAGFMRTFG IDEPMGCYDD IEHADAFVLW GSNMAEMHPI LWTRITDRKL SNKGVKVAVL 

       250        260        270        280        290        300 
STFEHRSYEL ADIPMIFTPQ TDLAILNYIA NYIIQNGKVN QAFVDKNVNF KKSATDIGYG 

       310        320        330        340        350        360 
LRPTHALEKN ATSNGYPDAD GKPKGDTGKS DPITFDEFKK FVSEYTVEKV SKLSGVAEKD 

       370        380        390        400        410        420 
LKALAELYAD PKVKVISFWT MGFNQHTRGT WANNLCYNIH LLTGKISEPG NSPFSLTGQP 

       430        440        450        460        470        480 
SACGTAREVG TFSHRLPADM VVTNPEHRKH TEELWGLPEG TIPDKVGYHA VAMARALKDG 

       490        500        510        520        530        540 
KVNFYWQQCN NNMQAGPNIN EELYPGWRKP ENFIVVSDPY PTVSAMAADL ILPTAMWVEK 

       550        560        570        580        590        600 
EGAYGNAERR TQFWRQQVKA PGEARSDLWQ LMEFSKRFKV EEVWPAELVA KAPKLKGKTL 

       610        620        630        640        650        660 
FDVLYANGVV NKYKLNETAA GFDNDDSKLI GFYIQKGLFE EYASFGRGHG HDLAPFDSYH 

       670        680        690        700        710        720 
QARGLRWPVV GGKETLWRFR EGYDPYVKKG EGVKFYGHKD GKAVIFALPY QPPAESPDKE 

       730        740        750        760        770        780 
FDMWLSTGRV LEHWHTGTMT RRVPELYKAF PDAVVFMHPD DAKARGLQRG MEVKVASRRG 

       790        800        810        820        830 
EIQLRVETRG RNKPPRGLVF IPFFDAGRLV NKLTLDATCP ISKETDYKKC AVKVTKV 

« Hide

References

[1]"Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation."
Salinero K.K., Keller K., Feil W.S., Feil H., Trong S., Di Bartolo G., Lapidus A.
BMC Genomics 10:351-351(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RCB.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000089 Genomic DNA. Translation: AAZ48244.1.
RefSeqYP_286714.1. NC_007298.1.

3D structure databases

ProteinModelPortalQ47A87.
SMRQ47A87. Positions 35-836.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING159087.Daro_3515.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ48244; AAZ48244; Daro_3515.
GeneID3567380.
KEGGdar:Daro_3515.
PATRIC21605702. VBIDecAro89105_3494.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMADAFGFYI.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycDARO159087:GI5B-3590-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_DECAR
AccessionPrimary (citable) accession number: Q47A87
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families