Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Dechloromonas aromatica (strain RCB)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi47Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi79Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei81Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei148Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei173Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei381Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei385Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei491Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei540Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei567Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei803Substrate; via amide nitrogenUniRule annotation1
Binding sitei811Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei828Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciDARO159087:G1G4R-3630-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Daro_3515
OrganismiDechloromonas aromatica (strain RCB)
Taxonomic identifieri159087 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesAzonexaceaeDechloromonas
Proteomesi
  • UP000000550 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Tat-type signalUniRule annotationAdd BLAST31
ChainiPRO_000004598332 – 837Periplasmic nitrate reductaseUniRule annotationAdd BLAST806

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi159087.Daro_3515.

Structurei

3D structure databases

ProteinModelPortaliQ47A87.
SMRiQ47A87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 934Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 217Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni241 – 245Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni260 – 262Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni517 – 518Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni727 – 736Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000031441.
KOiK02567.
OMAiTQHWRQQ.
OrthoDBiPOG091H060P.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct_NapA. 1 hit.
InterProiView protein in InterPro
IPR009010. Asp_de-COase-like_dom_sf.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
PANTHERiPTHR11615:SF123. PTHR11615:SF123. 1 hit.
PfamiView protein in Pfam
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
SMARTiView protein in SMART
SM00926. Molybdop_Fe4S4. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiView protein in PROSITE
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q47A87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLNRRDFIK ANAAAAAISA AGLSVPGAAV AQGKDEIRWD KAACRFCGTG
60 70 80 90 100
CGVLVGTQDG RVVATQGDPD APVNRGLNCI KGYFLSKIMY GADRLKTPML
110 120 130 140 150
RMKDGKYDKN GEFTPISWTK AFDIMEEKAK ATMKAKGPNG LAMFGSGQWT
160 170 180 190 200
IWEGYAASKL MKAGFRTNNL DPNARHCMAS AVAGFMRTFG IDEPMGCYDD
210 220 230 240 250
IEHADAFVLW GSNMAEMHPI LWTRITDRKL SNKGVKVAVL STFEHRSYEL
260 270 280 290 300
ADIPMIFTPQ TDLAILNYIA NYIIQNGKVN QAFVDKNVNF KKSATDIGYG
310 320 330 340 350
LRPTHALEKN ATSNGYPDAD GKPKGDTGKS DPITFDEFKK FVSEYTVEKV
360 370 380 390 400
SKLSGVAEKD LKALAELYAD PKVKVISFWT MGFNQHTRGT WANNLCYNIH
410 420 430 440 450
LLTGKISEPG NSPFSLTGQP SACGTAREVG TFSHRLPADM VVTNPEHRKH
460 470 480 490 500
TEELWGLPEG TIPDKVGYHA VAMARALKDG KVNFYWQQCN NNMQAGPNIN
510 520 530 540 550
EELYPGWRKP ENFIVVSDPY PTVSAMAADL ILPTAMWVEK EGAYGNAERR
560 570 580 590 600
TQFWRQQVKA PGEARSDLWQ LMEFSKRFKV EEVWPAELVA KAPKLKGKTL
610 620 630 640 650
FDVLYANGVV NKYKLNETAA GFDNDDSKLI GFYIQKGLFE EYASFGRGHG
660 670 680 690 700
HDLAPFDSYH QARGLRWPVV GGKETLWRFR EGYDPYVKKG EGVKFYGHKD
710 720 730 740 750
GKAVIFALPY QPPAESPDKE FDMWLSTGRV LEHWHTGTMT RRVPELYKAF
760 770 780 790 800
PDAVVFMHPD DAKARGLQRG MEVKVASRRG EIQLRVETRG RNKPPRGLVF
810 820 830
IPFFDAGRLV NKLTLDATCP ISKETDYKKC AVKVTKV
Length:837
Mass (Da):93,395
Last modified:September 13, 2005 - v1
Checksum:iE16F8200D0517622
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ48244.1.
RefSeqiWP_011289240.1. NC_007298.1.

Genome annotation databases

EnsemblBacteriaiAAZ48244; AAZ48244; Daro_3515.
KEGGidar:Daro_3515.

Similar proteinsi

Entry informationi

Entry nameiNAPA_DECAR
AccessioniPrimary (citable) accession number: Q47A87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: September 13, 2005
Last modified: March 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome