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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Dechloromonas aromatica (strain RCB)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciDARO159087:GI5B-3590-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Daro_3515
OrganismiDechloromonas aromatica (strain RCB)
Taxonomic identifieri159087 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeDechloromonas
ProteomesiUP000000550 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
BLAST
Chaini32 – 837806Periplasmic nitrate reductasePRO_0000045983Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi159087.Daro_3515.

Structurei

3D structure databases

ProteinModelPortaliQ47A87.
SMRiQ47A87. Positions 35-836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiCSERRYS.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q47A87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLNRRDFIK ANAAAAAISA AGLSVPGAAV AQGKDEIRWD KAACRFCGTG
60 70 80 90 100
CGVLVGTQDG RVVATQGDPD APVNRGLNCI KGYFLSKIMY GADRLKTPML
110 120 130 140 150
RMKDGKYDKN GEFTPISWTK AFDIMEEKAK ATMKAKGPNG LAMFGSGQWT
160 170 180 190 200
IWEGYAASKL MKAGFRTNNL DPNARHCMAS AVAGFMRTFG IDEPMGCYDD
210 220 230 240 250
IEHADAFVLW GSNMAEMHPI LWTRITDRKL SNKGVKVAVL STFEHRSYEL
260 270 280 290 300
ADIPMIFTPQ TDLAILNYIA NYIIQNGKVN QAFVDKNVNF KKSATDIGYG
310 320 330 340 350
LRPTHALEKN ATSNGYPDAD GKPKGDTGKS DPITFDEFKK FVSEYTVEKV
360 370 380 390 400
SKLSGVAEKD LKALAELYAD PKVKVISFWT MGFNQHTRGT WANNLCYNIH
410 420 430 440 450
LLTGKISEPG NSPFSLTGQP SACGTAREVG TFSHRLPADM VVTNPEHRKH
460 470 480 490 500
TEELWGLPEG TIPDKVGYHA VAMARALKDG KVNFYWQQCN NNMQAGPNIN
510 520 530 540 550
EELYPGWRKP ENFIVVSDPY PTVSAMAADL ILPTAMWVEK EGAYGNAERR
560 570 580 590 600
TQFWRQQVKA PGEARSDLWQ LMEFSKRFKV EEVWPAELVA KAPKLKGKTL
610 620 630 640 650
FDVLYANGVV NKYKLNETAA GFDNDDSKLI GFYIQKGLFE EYASFGRGHG
660 670 680 690 700
HDLAPFDSYH QARGLRWPVV GGKETLWRFR EGYDPYVKKG EGVKFYGHKD
710 720 730 740 750
GKAVIFALPY QPPAESPDKE FDMWLSTGRV LEHWHTGTMT RRVPELYKAF
760 770 780 790 800
PDAVVFMHPD DAKARGLQRG MEVKVASRRG EIQLRVETRG RNKPPRGLVF
810 820 830
IPFFDAGRLV NKLTLDATCP ISKETDYKKC AVKVTKV
Length:837
Mass (Da):93,395
Last modified:September 13, 2005 - v1
Checksum:iE16F8200D0517622
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ48244.1.
RefSeqiWP_011289240.1. NC_007298.1.
YP_286714.1. NC_007298.1.

Genome annotation databases

EnsemblBacteriaiAAZ48244; AAZ48244; Daro_3515.
KEGGidar:Daro_3515.
PATRICi21605702. VBIDecAro89105_3494.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000089 Genomic DNA. Translation: AAZ48244.1.
RefSeqiWP_011289240.1. NC_007298.1.
YP_286714.1. NC_007298.1.

3D structure databases

ProteinModelPortaliQ47A87.
SMRiQ47A87. Positions 35-836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi159087.Daro_3515.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ48244; AAZ48244; Daro_3515.
KEGGidar:Daro_3515.
PATRICi21605702. VBIDecAro89105_3494.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiCSERRYS.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciDARO159087:GI5B-3590-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation."
    Salinero K.K., Keller K., Feil W.S., Feil H., Trong S., Di Bartolo G., Lapidus A.
    BMC Genomics 10:351-351(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RCB.

Entry informationi

Entry nameiNAPA_DECAR
AccessioniPrimary (citable) accession number: Q47A87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: September 13, 2005
Last modified: May 27, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.