Reviewed, UniProtKB/Swiss-Prot Q47A87 (NAPA_DECAR)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	Daro_3515

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas

Protein attributes

Sequence length	837 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Tat-type signal Potential
	Chain	32 – 837	806	Periplasmic nitrate reductase HAMAP MF_01630		PRO_0000045983
Sites
	Metal binding	44	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	47	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	79	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47A87-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: E16F8200D0517622	FASTA	837	93,395
	10 20 30 40 50 60 MKLNRRDFIK ANAAAAAISA AGLSVPGAAV AQGKDEIRWD KAACRFCGTG CGVLVGTQDG 70 80 90 100 110 120 RVVATQGDPD APVNRGLNCI KGYFLSKIMY GADRLKTPML RMKDGKYDKN GEFTPISWTK 130 140 150 160 170 180 AFDIMEEKAK ATMKAKGPNG LAMFGSGQWT IWEGYAASKL MKAGFRTNNL DPNARHCMAS 190 200 210 220 230 240 AVAGFMRTFG IDEPMGCYDD IEHADAFVLW GSNMAEMHPI LWTRITDRKL SNKGVKVAVL 250 260 270 280 290 300 STFEHRSYEL ADIPMIFTPQ TDLAILNYIA NYIIQNGKVN QAFVDKNVNF KKSATDIGYG 310 320 330 340 350 360 LRPTHALEKN ATSNGYPDAD GKPKGDTGKS DPITFDEFKK FVSEYTVEKV SKLSGVAEKD 370 380 390 400 410 420 LKALAELYAD PKVKVISFWT MGFNQHTRGT WANNLCYNIH LLTGKISEPG NSPFSLTGQP 430 440 450 460 470 480 SACGTAREVG TFSHRLPADM VVTNPEHRKH TEELWGLPEG TIPDKVGYHA VAMARALKDG 490 500 510 520 530 540 KVNFYWQQCN NNMQAGPNIN EELYPGWRKP ENFIVVSDPY PTVSAMAADL ILPTAMWVEK 550 560 570 580 590 600 EGAYGNAERR TQFWRQQVKA PGEARSDLWQ LMEFSKRFKV EEVWPAELVA KAPKLKGKTL 610 620 630 640 650 660 FDVLYANGVV NKYKLNETAA GFDNDDSKLI GFYIQKGLFE EYASFGRGHG HDLAPFDSYH 670 680 690 700 710 720 QARGLRWPVV GGKETLWRFR EGYDPYVKKG EGVKFYGHKD GKAVIFALPY QPPAESPDKE 730 740 750 760 770 780 FDMWLSTGRV LEHWHTGTMT RRVPELYKAF PDAVVFMHPD DAKARGLQRG MEVKVASRRG 790 800 810 820 830 EIQLRVETRG RNKPPRGLVF IPFFDAGRLV NKLTLDATCP ISKETDYKKC AVKVTKV

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000089 Genomic DNA. Translation: AAZ48244.1.
RefSeq	YP_286714.1.
3D structure databases
SMR	Q47A87. Positions 35-836.
ModBase	Search...
Protein-protein interaction databases
STRING	Q47A87.
Genome annotation databases
GeneID	3567380.
GenomeReviews	Gene locus Daro_3515 in contig CP000089_GR.
KEGG	dar:Daro_3515.
NMPDR	fig|159087.4.peg.2719.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q47A87.
OMA	ERRTQAW.
Enzyme and pathway databases
BioCyc	DARO159087:DARO_3515-MON.
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_DECAR

Accession

Primary (citable) accession number: Q47A87

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)