Reviewed, UniProtKB/Swiss-Prot Q479A2 (RNPH_DECAR)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	Daro_3851

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease PH HAMAP MF_00564		PRO_1000024800

Sequences

	Sequence		Length	Mass (Da)
	Q479A2-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 7B447A21130B4C1E	FASTA	238	25,696
	10 20 30 40 50 60 MRPSQRQPDQ LRTVRITRNF TRHAEGSVLI EMGDTRVLCT ASVEENVPPF LRGKGQGWVT 70 80 90 100 110 120 AEYGMLPRAT HTRSSREAAK GKQTGRTQEI QRLIGRALRS VIDLKALGER QITLDCDVLQ 130 140 150 160 170 180 ADGGTRCASI TGAWVALYEA CDKLVQAGKL PANPVRDHVA AISVGIYKGA PVLDLDYPED 190 200 210 220 230 SDCDTDMNVV MTGAGGIVEI QGTAEGEPFT REQMSVLVDL ATLGVRHLVA AQQSALAA

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000089 Genomic DNA. Translation: AAZ48579.1.
RefSeq	YP_287049.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3568022.
GenomeReviews	Gene locus Daro_3851 in contig CP000089_GR.
KEGG	dar:Daro_3851.
NMPDR	fig|159087.4.peg.2595.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q479A2.
OMA	Q479A2. YAMLPRA.
Enzyme and pathway databases
BioCyc	DARO159087:DARO_3851-MON.
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR018336. Ribonuclease-PH_CS. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_DECAR

Accession

Primary (citable) accession number: Q479A2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)