Reviewed, UniProtKB/Swiss-Prot Q47951 (DHE3_PYREN)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase
      Short name=GDH
    EC=1.4.1.3

Gene names

Name:

gdhA

Organism

Pyrococcus endeavori

Taxonomic identifier

39456 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NAD(P)(+) = 2-oxoglutarate + NH(3) + NAD(P)H.
Subunit structure	Homohexamer.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Glutamate dehydrogenase		PRO_0000182756
Regions
	Nucleotide binding	220 – 226	7	NAD Potential
Sites
	Active site	105	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47951-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 26C571CC5DEF85CB	FASTA	420	47,141
	10 20 30 40 50 60 MVEQDPFEIA VKQLERAAQY MKISEEALEF LKRPQRIVEV TIPVEMDDGT VKVFTGFRVQ 70 80 90 100 110 120 YNWARGPTKG GIRWHPEETL STVKALAAWM TWKTAVMDLP YGGGKGGIIV DPKKLSDREK 130 140 150 160 170 180 ERLARGYIRA IYDVISPYED IPAPDVYTNP QIMAWMMDEY EAISRRKTPA FGIITGKPLS 190 200 210 220 230 240 IGGSLGRNEA TARGASYTIR EARKVLGWGD LKGKTIAIQG YGNAGYYLAK IMSEDYGMKV 250 260 270 280 290 300 VAVSDSKGGI YNPDGLNADE VLKWKQEHGS VKDFPGATNI TNEELLELEV DVLAPAAIEE 310 320 330 340 350 360 VITKKNADNI KAKIVAEVAN GPVTPEADEI LFEKGILQIP DFLCNAGGVT VSYFEWVQNI 370 380 390 400 410 420 TGYYWTLEEV REKLDKKMTK AFYDVYNTAK EKNIHMRDAD YVVAVQRVYQ AMLDRGWVKH

References

[1]

"Characterization, cloning, and in vitro expression of the extremely thermostable glutamate dehydrogenase from the hyperthermophilic Archaeon, ES4." Diruggiero J., Robb F.T., Jagus R., Klump H.H., Borges K.M., Kessel M., Mai X., Adams M.W.W.A. J. Biol. Chem. 268:17767-17774(1993) [PubMed: 8349661] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Strain: ES4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12408 Genomic DNA. Translation: AAA64795.1.
PIR	A47410.
3D structure databases
HSSP	HSSP built from PDB template 1GTM based on UniProtKB P80319.
SMR	Q47951. Positions 5-420.
ModBase	Search...
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR014362. Glu_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE3_PYREN

Accession

Primary (citable) accession number: Q47951

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)