Reviewed, UniProtKB/Swiss-Prot Q47907 (CCA_FRANO)

Names and origin

Protein names

Recommended name:
    tRNA nucleotidyltransferase
    EC=2.7.7.25
Alternative name(s):
    tRNA adenylyltransferase
    tRNA CCA-pyrophosphorylase
    CCA-adding enzyme

Gene names

Name:

cca

Organism

Francisella novicida

Taxonomic identifier

264 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	84 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme carries out synthesis of the tRNA CCA terminus without the direction of a template using the multiple accepting and donating subsites within its active site By similarity.
Catalytic activity	ATP + tRNA(n) = diphosphate + tRNA(n+1).
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›84	›84	tRNA nucleotidyltransferase		PRO_0000139088
Regions
	Nucleotide binding	68 – 73	6	ATP Potential
Experimental info
	Non-terminal residue	84	1

Sequences

	Sequence		Length	Mass (Da)
	Q47907-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2C8625AF99C84095	FASTA	84	9,527
	10 20 30 40 50 60 MKFYLVGGAV RDMLLGITPK DKDWVVVGAT EDEMLANGFI KIAANFPVFI HPQTKQEYAL 70 80 ARSEKKTRNG YHGFEVNFSK YITL

References

[1]	"Serum-sensitive mutation of Francisella novicida: association with an ABC transporter gene." Mdluli K.E., Anthony L.S., Baron G.S., McDonald M.K., Myltseva S.V., Nano F.E. Microbiology 140:3309-3318(1994) [PubMed: 7881549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: U112.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L17003 Genomic DNA. Translation: AAD15236.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002646. PolyA_pol_reg. [Graphical view]
Pfam	PF01743. PolyA_pol. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCA_FRANO

Accession

Primary (citable) accession number: Q47907

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)