Q47862 (BIOB_ESCVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:

bioB

Organism

Escherichia vulneris

Taxonomic identifier

566 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Biotin synthase HAMAP-Rule MF_01694		PRO_0000185553
Sites
	Metal binding	53	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	57	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	60	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	97	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	128	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	188	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	260	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47862 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: B38A66F7E572C7EF	FASTA	346	38,714
	10 20 30 40 50 60 MAHHARWTLS QVTELFDKPL LDLLFEAQTI HRQHFDPRQV QVSTLLSIKT GACPEDCKYC 70 80 90 100 110 120 PQSSRYKTGL ETERLMEVEQ VLDSARKAKN AGSTRFCMGA AWKNPHERDM PYLEQMVQGV 130 140 150 160 170 180 KAMGLEACMT LGTLDETQAQ RLASAGLDYY NHNLDTSPEF YGNIITTRTY QERLDTLDKV 190 200 210 220 230 240 RDAGIKVCSG GIVGLGETVK DRAGLLLQLA NLPTPPESVP INMLVKVKGT PLADNDDVDA 250 260 270 280 290 300 FDFIRTIAVA RIMMPTSFVR LSAGREQMNE QTQAMCFMAG ANSIFYGCKL LTTPNPEEDK 310 320 330 340 DVQLFRKLGL NPQQTDVMTG DNEQQQKLEE QIFNADTDQF YNAAAL

References

[1]	"Isolation and characterization of the Erwinia herbicola bio operon and the sequences of the bioA and bioB genes." Wu C.-H., Chen H.-Y., Shiuan D. Gene 174:251-258(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 39368 / Eho10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38648 Genomic DNA. Translation: AAC44534.1.
PIR	JC5006.
3D structure databases
ProteinModelPortal	Q47862.
SMR	Q47862. Positions 3-315.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_ESCVU

Accession

Primary (citable) accession number: Q47862

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program