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Q47839

- COPY_ENTHA

UniProt

Q47839 - COPY_ENTHA

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Protein

Transcriptional repressor CopY

Gene

copY

Organism
Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a copper responsive repressor. Binds to DNA in complex with Zn2+, repressing the transcription of the copYZAB operon. Exchange of the bound zinc by two copper ions delivered by CopZ Cu1+-bound form causes release of CopY from the promoter, leading to the transcription of the operon.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi133 – 1331ZincSequence Analysis
Metal bindingi135 – 1351ZincSequence Analysis
Metal bindingi140 – 1401ZincSequence Analysis
Metal bindingi142 – 1421ZincSequence Analysis

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: InterPro
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Copper, DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor CopY
Alternative name(s):
CopAB ATPases metal-fist type repressor
Gene namesi
Name:copY
Ordered Locus Names:EHR_09075
OrganismiEnterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
Taxonomic identifieri768486 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000002895: Chromosome

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 145145Transcriptional repressor CopYPRO_0000062795Add
BLAST

Expressioni

Inductioni

By copper.1 Publication

Interactioni

Subunit structurei

Homodimer. Interacts with CopZ Cu1+-bound form via a charge-based interaction.3 Publications

Protein-protein interaction databases

MINTiMINT-86878.

Structurei

3D structure databases

ProteinModelPortaliQ47839.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005650. BlaI_family.
IPR014071. Cu_transp_CopY/TcrY.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03965. Penicillinase_R. 1 hit.
[Graphical view]
PIRSFiPIRSF019455. CopR_AtkY. 1 hit.
TIGRFAMsiTIGR02698. CopY_TcrY. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47839-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEKRVLIKI SDSEWEVMRV IWTLGQANAQ QITQILADSM DWKVATVKTL
60 70 80 90 100
LGRLVKKEAL WTEQEGKKFI YHPAVSEMEN VRSATENLFS HICAKRVGAT
110 120 130 140
IADLVEEATL TQEDIQQIMK QLNKKEPVET IECNCIPGQC ECKKQ
Length:145
Mass (Da):16,577
Last modified:November 1, 1996 - v1
Checksum:iBC825992EE968868
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46807 Genomic DNA. Translation: CAA86835.1.
CP003504 Genomic DNA. Translation: AFM70727.1.
PIRiA56085.
RefSeqiWP_010737926.1. NZ_KB946231.1.
YP_006487618.1. NC_018081.1.

Genome annotation databases

EnsemblBacteriaiAFM70727; AFM70727; EHR_09075.
GeneIDi13177744.
KEGGiehr:EHR_09075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46807 Genomic DNA. Translation: CAA86835.1 .
CP003504 Genomic DNA. Translation: AFM70727.1 .
PIRi A56085.
RefSeqi WP_010737926.1. NZ_KB946231.1.
YP_006487618.1. NC_018081.1.

3D structure databases

ProteinModelPortali Q47839.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-86878.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFM70727 ; AFM70727 ; EHR_09075 .
GeneIDi 13177744.
KEGGi ehr:EHR_09075.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR005650. BlaI_family.
IPR014071. Cu_transp_CopY/TcrY.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF03965. Penicillinase_R. 1 hit.
[Graphical view ]
PIRSFi PIRSF019455. CopR_AtkY. 1 hit.
TIGRFAMsi TIGR02698. CopY_TcrY. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two trans-acting metalloregulatory proteins controlling expression of the copper-ATPases of Enterococcus hirae."
    Odermatt A., Solioz M.
    J. Biol. Chem. 270:4349-4354(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A REPRESSOR.
    Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
  2. "Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis."
    Gaechter T., Wunderlin C., Schmidheini T., Solioz M.
    J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
  3. "CopY is a copper-inducible repressor of the Enterococcus hirae copper ATPases."
    Strausak D., Solioz M.
    J. Biol. Chem. 272:8932-8936(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A REPRESSOR, DNA-BINDING, SUBUNIT.
    Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
  4. "Effects of promoter mutations on the in vivo regulation of the cop operon of Enterococcus hirae by copper(I) and copper(II)."
    Wunderli-Ye H., Solioz M.
    Biochem. Biophys. Res. Commun. 259:443-449(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY COPPER.
    Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
  5. "The Enterococcus hirae copper chaperone CopZ delivers copper(I) to the CopY repressor."
    Cobine P.A., Wickramasinghe W.A., Harrison M.D., Weber T., Solioz M., Dameron C.T.
    FEBS Lett. 445:27-30(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH COPZ.
    Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
  6. "Copper transfer from the Cu(I) chaperone, CopZ, to the repressor, Zn(II)CopY: metal coordination environments and protein interactions."
    Cobine P.A., George G.N., Jones C.E., Wickramasinghe W.A., Solioz M., Dameron C.T.
    Biochemistry 41:5822-5829(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH COPZ.
    Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.

Entry informationi

Entry nameiCOPY_ENTHA
AccessioniPrimary (citable) accession number: Q47839
Secondary accession number(s): I6SZ22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3