Q47839 (COPY_ENTHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional repressor CopY

Alternative name(s):
CopAB ATPases metal-fist type repressor

Gene names

Name:	copY
Ordered Locus Names:	EHR_09075

Organism

Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258) [Complete proteome] [HAMAP]

Taxonomic identifier

768486 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Enterococcaceae › Enterococcus › Enterococcus hirae

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a copper responsive repressor. Binds to DNA in complex with Zn2+, repressing the transcription of the copYZAB operon. Exchange of the bound zinc by two copper ions delivered by CopZ Cu1+-bound form causes release of CopY from the promoter, leading to the transcription of the operon. Ref.1 Ref.3 Ref.5 Ref.6
Subunit structure	Homodimer. Interacts with CopZ Cu1+-bound form via a charge-based interaction. Ref.3 Ref.5 Ref.6
Subcellular location	Cytoplasm Probable.
Induction	By copper. Ref.4
Sequence similarities	Belongs to the BlaI transcriptional regulatory family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	Copper DNA-binding Metal-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 145	145	Transcriptional repressor CopY		PRO_0000062795
Sites
	Metal binding	133	1	Zinc Potential
	Metal binding	135	1	Zinc Potential
	Metal binding	140	1	Zinc Potential
	Metal binding	142	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q47839 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BC825992EE968868	FASTA	145	16,577
	10 20 30 40 50 60 MEEKRVLIKI SDSEWEVMRV IWTLGQANAQ QITQILADSM DWKVATVKTL LGRLVKKEAL 70 80 90 100 110 120 WTEQEGKKFI YHPAVSEMEN VRSATENLFS HICAKRVGAT IADLVEEATL TQEDIQQIMK 130 140 QLNKKEPVET IECNCIPGQC ECKKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two trans-acting metalloregulatory proteins controlling expression of the copper-ATPases of Enterococcus hirae." Odermatt A., Solioz M. J. Biol. Chem. 270:4349-4354(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A REPRESSOR. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[2]	"Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis." Gaechter T., Wunderlin C., Schmidheini T., Solioz M. J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[3]	"CopY is a copper-inducible repressor of the Enterococcus hirae copper ATPases." Strausak D., Solioz M. J. Biol. Chem. 272:8932-8936(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A REPRESSOR, DNA-BINDING, SUBUNIT. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[4]	"Effects of promoter mutations on the in vivo regulation of the cop operon of Enterococcus hirae by copper(I) and copper(II)." Wunderli-Ye H., Solioz M. Biochem. Biophys. Res. Commun. 259:443-449(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY COPPER. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[5]	"The Enterococcus hirae copper chaperone CopZ delivers copper(I) to the CopY repressor." Cobine P.A., Wickramasinghe W.A., Harrison M.D., Weber T., Solioz M., Dameron C.T. FEBS Lett. 445:27-30(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH COPZ. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[6]	"Copper transfer from the Cu(I) chaperone, CopZ, to the repressor, Zn(II)CopY: metal coordination environments and protein interactions." Cobine P.A., George G.N., Jones C.E., Wickramasinghe W.A., Solioz M., Dameron C.T. Biochemistry 41:5822-5829(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH COPZ. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46807 Genomic DNA. Translation: CAA86835.1. CP003504 Genomic DNA. Translation: AFM70727.1.
PIR	A56085.
RefSeq	YP_006487618.1. NC_018081.1.
3D structure databases
ProteinModelPortal	Q47839.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	13177744.
KEGG	ehr:EHR_09075.
Organism-specific databases
CMR	Search...
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR005650. BlaI_family. IPR014071. Cu_transp_CopY/TcrY. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF03965. Pencillinase_R. 1 hit. [Graphical view]
PIRSF	PIRSF019455. CopR_AtkY. 1 hit.
TIGRFAMs	TIGR02698. CopY_TcrY. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COPY_ENTHA

Accession

Primary (citable) accession number: Q47839
Secondary accession number(s): I6SZ22

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program