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Q47839 (COPY_ENTHA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional repressor CopY
Alternative name(s):
CopAB ATPases metal-fist type repressor
Gene names
Name:copY
Ordered Locus Names:EHR_09075
OrganismEnterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258) [Complete proteome] [HAMAP]
Taxonomic identifier768486 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length145 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a copper responsive repressor. Binds to DNA in complex with Zn2+, repressing the transcription of the copYZAB operon. Exchange of the bound zinc by two copper ions delivered by CopZ Cu1+-bound form causes release of CopY from the promoter, leading to the transcription of the operon. Ref.1 Ref.3 Ref.5 Ref.6

Subunit structure

Homodimer. Interacts with CopZ Cu1+-bound form via a charge-based interaction. Ref.3 Ref.5 Ref.6

Subcellular location

Cytoplasm Probable.

Induction

By copper. Ref.4

Sequence similarities

Belongs to the BlaI transcriptional regulatory family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
   LigandCopper
DNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processnegative regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 145145Transcriptional repressor CopY
PRO_0000062795

Sites

Metal binding1331Zinc Potential
Metal binding1351Zinc Potential
Metal binding1401Zinc Potential
Metal binding1421Zinc Potential

Sequences

Sequence LengthMass (Da)Tools
Q47839 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BC825992EE968868

FASTA14516,577
        10         20         30         40         50         60 
MEEKRVLIKI SDSEWEVMRV IWTLGQANAQ QITQILADSM DWKVATVKTL LGRLVKKEAL 

        70         80         90        100        110        120 
WTEQEGKKFI YHPAVSEMEN VRSATENLFS HICAKRVGAT IADLVEEATL TQEDIQQIMK 

       130        140 
QLNKKEPVET IECNCIPGQC ECKKQ 

« Hide

References

« Hide 'large scale' references
[1]"Two trans-acting metalloregulatory proteins controlling expression of the copper-ATPases of Enterococcus hirae."
Odermatt A., Solioz M.
J. Biol. Chem. 270:4349-4354(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A REPRESSOR.
Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[2]"Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis."
Gaechter T., Wunderlin C., Schmidheini T., Solioz M.
J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[3]"CopY is a copper-inducible repressor of the Enterococcus hirae copper ATPases."
Strausak D., Solioz M.
J. Biol. Chem. 272:8932-8936(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A REPRESSOR, DNA-BINDING, SUBUNIT.
Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[4]"Effects of promoter mutations on the in vivo regulation of the cop operon of Enterococcus hirae by copper(I) and copper(II)."
Wunderli-Ye H., Solioz M.
Biochem. Biophys. Res. Commun. 259:443-449(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY COPPER.
Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[5]"The Enterococcus hirae copper chaperone CopZ delivers copper(I) to the CopY repressor."
Cobine P.A., Wickramasinghe W.A., Harrison M.D., Weber T., Solioz M., Dameron C.T.
FEBS Lett. 445:27-30(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH COPZ.
Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[6]"Copper transfer from the Cu(I) chaperone, CopZ, to the repressor, Zn(II)CopY: metal coordination environments and protein interactions."
Cobine P.A., George G.N., Jones C.E., Wickramasinghe W.A., Solioz M., Dameron C.T.
Biochemistry 41:5822-5829(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH COPZ.
Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46807 Genomic DNA. Translation: CAA86835.1.
CP003504 Genomic DNA. Translation: AFM70727.1.
PIRA56085.
RefSeqYP_006487618.1. NC_018081.1.

3D structure databases

ProteinModelPortalQ47839.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-86878.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFM70727; AFM70727; EHR_09075.
GeneID13177744.
KEGGehr:EHR_09075.

Organism-specific databases

CMRSearch...

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR005650. BlaI_family.
IPR014071. Cu_transp_CopY/TcrY.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF03965. Penicillinase_R. 1 hit.
[Graphical view]
PIRSFPIRSF019455. CopR_AtkY. 1 hit.
TIGRFAMsTIGR02698. CopY_TcrY. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCOPY_ENTHA
AccessionPrimary (citable) accession number: Q47839
Secondary accession number(s): I6SZ22
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families