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Q47839

- COPY_ENTHA

UniProt

Q47839 - COPY_ENTHA

Protein

Transcriptional repressor CopY

Gene

copY

Organism
Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts as a copper responsive repressor. Binds to DNA in complex with Zn2+, repressing the transcription of the copYZAB operon. Exchange of the bound zinc by two copper ions delivered by CopZ Cu1+-bound form causes release of CopY from the promoter, leading to the transcription of the operon.4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi133 – 1331ZincSequence Analysis
    Metal bindingi135 – 1351ZincSequence Analysis
    Metal bindingi140 – 1401ZincSequence Analysis
    Metal bindingi142 – 1421ZincSequence Analysis

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: InterPro
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Copper, DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional repressor CopY
    Alternative name(s):
    CopAB ATPases metal-fist type repressor
    Gene namesi
    Name:copY
    Ordered Locus Names:EHR_09075
    OrganismiEnterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
    Taxonomic identifieri768486 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    ProteomesiUP000002895: Chromosome

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 145145Transcriptional repressor CopYPRO_0000062795Add
    BLAST

    Expressioni

    Inductioni

    By copper.1 Publication

    Interactioni

    Subunit structurei

    Homodimer. Interacts with CopZ Cu1+-bound form via a charge-based interaction.3 Publications

    Protein-protein interaction databases

    MINTiMINT-86878.

    Structurei

    3D structure databases

    ProteinModelPortaliQ47839.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR005650. BlaI_family.
    IPR014071. Cu_transp_CopY/TcrY.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF03965. Penicillinase_R. 1 hit.
    [Graphical view]
    PIRSFiPIRSF019455. CopR_AtkY. 1 hit.
    TIGRFAMsiTIGR02698. CopY_TcrY. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q47839-1 [UniParc]FASTAAdd to Basket

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    MEEKRVLIKI SDSEWEVMRV IWTLGQANAQ QITQILADSM DWKVATVKTL    50
    LGRLVKKEAL WTEQEGKKFI YHPAVSEMEN VRSATENLFS HICAKRVGAT 100
    IADLVEEATL TQEDIQQIMK QLNKKEPVET IECNCIPGQC ECKKQ 145
    Length:145
    Mass (Da):16,577
    Last modified:November 1, 1996 - v1
    Checksum:iBC825992EE968868
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46807 Genomic DNA. Translation: CAA86835.1.
    CP003504 Genomic DNA. Translation: AFM70727.1.
    PIRiA56085.
    RefSeqiWP_010737926.1. NZ_KB946231.1.
    YP_006487618.1. NC_018081.1.

    Genome annotation databases

    EnsemblBacteriaiAFM70727; AFM70727; EHR_09075.
    GeneIDi13177744.
    KEGGiehr:EHR_09075.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46807 Genomic DNA. Translation: CAA86835.1 .
    CP003504 Genomic DNA. Translation: AFM70727.1 .
    PIRi A56085.
    RefSeqi WP_010737926.1. NZ_KB946231.1.
    YP_006487618.1. NC_018081.1.

    3D structure databases

    ProteinModelPortali Q47839.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-86878.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFM70727 ; AFM70727 ; EHR_09075 .
    GeneIDi 13177744.
    KEGGi ehr:EHR_09075.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR005650. BlaI_family.
    IPR014071. Cu_transp_CopY/TcrY.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF03965. Penicillinase_R. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF019455. CopR_AtkY. 1 hit.
    TIGRFAMsi TIGR02698. CopY_TcrY. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Two trans-acting metalloregulatory proteins controlling expression of the copper-ATPases of Enterococcus hirae."
      Odermatt A., Solioz M.
      J. Biol. Chem. 270:4349-4354(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A REPRESSOR.
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
    2. "Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis."
      Gaechter T., Wunderlin C., Schmidheini T., Solioz M.
      J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
    3. "CopY is a copper-inducible repressor of the Enterococcus hirae copper ATPases."
      Strausak D., Solioz M.
      J. Biol. Chem. 272:8932-8936(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A REPRESSOR, DNA-BINDING, SUBUNIT.
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
    4. "Effects of promoter mutations on the in vivo regulation of the cop operon of Enterococcus hirae by copper(I) and copper(II)."
      Wunderli-Ye H., Solioz M.
      Biochem. Biophys. Res. Commun. 259:443-449(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY COPPER.
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
    5. "The Enterococcus hirae copper chaperone CopZ delivers copper(I) to the CopY repressor."
      Cobine P.A., Wickramasinghe W.A., Harrison M.D., Weber T., Solioz M., Dameron C.T.
      FEBS Lett. 445:27-30(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH COPZ.
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
    6. "Copper transfer from the Cu(I) chaperone, CopZ, to the repressor, Zn(II)CopY: metal coordination environments and protein interactions."
      Cobine P.A., George G.N., Jones C.E., Wickramasinghe W.A., Solioz M., Dameron C.T.
      Biochemistry 41:5822-5829(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH COPZ.
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.

    Entry informationi

    Entry nameiCOPY_ENTHA
    AccessioniPrimary (citable) accession number: Q47839
    Secondary accession number(s): I6SZ22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3