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Q47827 (DDL_ENTHA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:EHR_03915
OrganismEnterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258) [Complete proteome] [HAMAP]
Taxonomic identifier768486 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177823

Regions

Domain136 – 341206ATP-grasp
Nucleotide binding169 – 22456ATP By similarity

Sites

Metal binding2951Magnesium or manganese 1 By similarity
Metal binding3081Magnesium or manganese 1 By similarity
Metal binding3081Magnesium or manganese 2 By similarity
Metal binding3101Magnesium or manganese 2 By similarity

Experimental info

Sequence conflict3551E → N in AAB17904. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q47827 [UniParc].

Last modified January 9, 2013. Version 2.
Checksum: 225CF997F0A979F1

FASTA35840,143
        10         20         30         40         50         60 
MKITLLYGGR SAEHDVSVLS AFSVLNAIYY TYYQVQLIFI SKEGQWVKGP LLTEKPTSKE 

        70         80         90        100        110        120 
DLHLTWDPSG KVTDGFTGRV INPGEIKEEG TIVFPVLHGP NGEDGTIQGF LETLNLPYVG 

       130        140        150        160        170        180 
AGVLTSACAM DKIMTKYILQ AAGVPQVPYV PVLKNQWKEN PKKIFDQCEG SLLYPMFVKP 

       190        200        210        220        230        240 
ANMGSSVGIS KAENREELQN ALALAYQYDS RAIVEQGIEA REIEVAVLGN EDVRTTLPGE 

       250        260        270        280        290        300 
VVKDVAFYDY DAKYINNKIE MQIPAEVPEE VYQKAQEYAK IAYTMLGGSG LSRCDFFLTN 

       310        320        330        340        350 
KNELFLNELN TMPGFTQFSM YPLLWENMGL KYGDLIEELI QLGINRFNQR QGFFEANE 

« Hide

References

« Hide 'large scale' references
[1]"Evolution of structure and substrate specificity in D-alanine:D-alanine ligases and related enzymes."
Evers S., Casadewall B., Charles M., Dutka-Malen S., Galimand M., Courvalin P.
J. Mol. Evol. 42:706-712(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[2]"Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis."
Gaechter T., Wunderlin C., Schmidheini T., Solioz M.
J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39788 Genomic DNA. Translation: AAB17904.1.
CP003504 Genomic DNA. Translation: AFM69753.1.
RefSeqYP_006486644.1. NC_018081.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFM69753; AFM69753; EHR_03915.
GeneID13178658.
KEGGehr:EHR_03915.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_ENTHA
AccessionPrimary (citable) accession number: Q47827
Secondary accession number(s): I6SB04
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 9, 2013
Last modified: February 19, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways