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Q47823 (DDL_ENTGA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
OrganismEnterococcus gallinarum
Taxonomic identifier1353 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length316 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›316›316D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177822

Regions

Domain129 – ›316›188ATP-grasp
Nucleotide binding162 – 21756ATP By similarity

Sites

Metal binding2881Magnesium or manganese 1 By similarity
Metal binding3011Magnesium or manganese 1 By similarity
Metal binding3011Magnesium or manganese 2 By similarity
Metal binding3031Magnesium or manganese 2 By similarity

Experimental info

Non-terminal residue3161

Sequences

Sequence LengthMass (Da)Tools
Q47823 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4FE5CDFDD716A8FB

FASTA31635,261
        10         20         30         40         50         60 
MKIILLYGGR SAEHDVSLLS AFSVVNAVYY NYYQVQLVMI TRDGQWLKGS LLTEAPTSKE 

        70         80         90        100        110        120 
VLNLTDSAYQ GTPIQPGEIK EEDAIVFPLL HGPNGEDGTI QGFLETIGMP YVGAGVLTSA 

       130        140        150        160        170        180 
CGMDKIMTKY ILQAAGIPQV PYVPVLKNYW KENPKKVFEQ CEGSLLYPMF IKPANMGSSV 

       190        200        210        220        230        240 
GITKAENREE LQNALQEAYR YDTRAIVEQG IEAREIEVAV LGNEDVRTTM PGEIVKDVAF 

       250        260        270        280        290        300 
YDYNSKYLDN KIEMQIPAQI PEETQAKAQE FAKKAYTMLG GSGLSRCDFF LTNKNELFLN 

       310 
ELNTIPALQA EFCRYP 

« Hide

References

[1]"Evolution of structure and substrate specificity in D-alanine:D-alanine ligases and related enzymes."
Evers S., Casadewall B., Charles M., Dutka-Malen S., Galimand M., Courvalin P.
J. Mol. Evol. 42:706-712(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BM4174.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39789 Genomic DNA. Translation: AAB17903.1.

3D structure databases

ProteinModelPortalQ47823.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_ENTGA
AccessionPrimary (citable) accession number: Q47823
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways