Q477F9 (PCP_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyrrolidone-carboxylate peptidase
EC=3.4.19.3

Alternative name(s):
5-oxoprolyl-peptidase
Pyroglutamyl-peptidase I
Short name=PGP-I
Short name=Pyrase

Gene names

Name:	pcp
Ordered Locus Names:	Reut_A0092

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus pinatubonensis

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes 5-oxoproline from various penultimate amino acid residues except L-proline By similarity. HAMAP-Rule MF_00417
Catalytic activity	Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro. HAMAP-Rule MF_00417
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_00417
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00417.
Sequence similarities	Belongs to the peptidase C15 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW pyroglutamyl-peptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Pyrrolidone-carboxylate peptidase HAMAP-Rule MF_00417		PRO_1000050136
Sites
	Active site	80	1	By similarity
	Active site	143	1	By similarity
	Active site	168	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q477F9 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: BFCD0854BD2CD482	FASTA	216	22,706
	10 20 30 40 50 60 MRTVLLTGFE PFENEPINPS WEAVRALDGE RIGDAVVVAR QLPCVFGAAI DGMAALLREL 70 80 90 100 110 120 KPAIAIAVGQ AGGRTEMSVE RVAINVDDAR IADNAGAQPI DTVIAAKGPA AYFSTLPIKA 130 140 150 160 170 180 IVRDMRAAGV PAAVSQTAGT FVCNHVFYGL MHALATPAGE GVRGGFIHIP YLPEQAARHP 190 200 210 GEASMSLESM VRGIRQAIAT TLATEVDVRE QGGQLH

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ59474.1.
RefSeq	YP_294318.1. NC_007347.1.
3D structure databases
ProteinModelPortal	Q477F9.
ModBase	Search...
Protein-protein interaction databases
STRING	264198.Reut_A0092.
Protein family/group databases
MEROPS	C15.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ59474; AAZ59474; Reut_A0092.
GeneID	3611158.
KEGG	reu:Reut_A0092.
PATRIC	20225617. VBIRalEut24049_0687.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2039.
HOGENOM	HOG000242641.
KO	K01304.
OMA	AYFTQLP.
ProtClustDB	PRK13197.
Enzyme and pathway databases
BioCyc	CPIN264198:GIW3-92-MONOMER.
Family and domain databases
Gene3D	3.40.630.20. 1 hit.
HAMAP	MF_00417. Pyrrolid_peptidase.
InterPro	IPR000816. Peptidase_C15. IPR016125. Peptidase_C15-like. [Graphical view]
PANTHER	PTHR23402. PTHR23402. 1 hit.
Pfam	PF01470. Peptidase_C15. 1 hit. [Graphical view]
PIRSF	PIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTS	PR00706. PYROGLUPTASE.
SUPFAM	SSF53182. Peptidase_C15-like. 1 hit.
TIGRFAMs	TIGR00504. pyro_pdase. 1 hit.
PROSITE	PS01334. PYRASE_CYS. 1 hit. PS01333. PYRASE_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCP_CUPPJ

Accession

Primary (citable) accession number: Q477F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program