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Protein
Submitted name:

AS-48 protein

Gene

as-48

Organism
Enterococcus faecalis (Streptococcus faecalis)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Protein family/group databases

TCDBi1.C.28.1.1. the bacteriocin as-48 cyclic polypeptide (bacteriocin as-48) family.

Names & Taxonomyi

Protein namesi
Submitted name:
AS-48 proteinImported
Submitted name:
AS-48AImported
Submitted name:
BacAImported
Submitted name:
Enterocin proproteinImported
Submitted name:
Peptide antibiotic AS-48Imported
Gene namesi
Name:as-48Imported
Synonyms:as48AImported, bacAImported
Encoded oniPlasmid BglIIImported
Plasmid pPD1Imported
Plasmid pTLRImported
OrganismiEnterococcus faecalis (Streptococcus faecalis)Imported
Taxonomic identifieri1351 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei60 – 8223HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E68NMR-A36-105[»]
1O82X-ray1.46A/B/C/D36-105[»]
1O83X-ray1.64A/B/C/D36-105[»]
1O84X-ray2.80A/B36-105[»]
4RGDX-ray1.20A/B36-105[»]
SMRiQ47765. Positions 36-105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysis

Family and domain databases

Gene3Di1.20.225.10. 1 hit.
InterProiIPR009086. Bacteriocin_AS48.
IPR020038. Bacteriocin_uberolysin_fam.
[Graphical view]
PfamiPF09221. Bacteriocin_IId. 1 hit.
[Graphical view]
SUPFAMiSSF47869. SSF47869. 1 hit.
TIGRFAMsiTIGR03651. circ_ocin_uber. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47765-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKENKFSKI FILMALSFLG LALFSASLQF LPIAHMAKEF GIPAAVAGTV
60 70 80 90 100
LNVVEAGGWV TTIVSILTAV GSGGLSLLAA AGRESIKAYL KKEIKKKGKR

AVIAW
Length:105
Mass (Da):11,076
Last modified:November 1, 1996 - v1
Checksum:iD8F313FFD1B5D2B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KF154975 Genomic DNA. Translation: AGQ55496.1.
KJ146793 Genomic DNA. Translation: AHL69645.1.
KT290268 Genomic DNA. Translation: AKU62176.1.
X79542 Genomic DNA. Translation: CAA56076.1.
Y12234 Genomic DNA. Translation: CAA72917.1.
PIRiS47168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KF154975 Genomic DNA. Translation: AGQ55496.1.
KJ146793 Genomic DNA. Translation: AHL69645.1.
KT290268 Genomic DNA. Translation: AKU62176.1.
X79542 Genomic DNA. Translation: CAA56076.1.
Y12234 Genomic DNA. Translation: CAA72917.1.
PIRiS47168.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E68NMR-A36-105[»]
1O82X-ray1.46A/B/C/D36-105[»]
1O83X-ray1.64A/B/C/D36-105[»]
1O84X-ray2.80A/B36-105[»]
4RGDX-ray1.20A/B36-105[»]
SMRiQ47765. Positions 36-105.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.28.1.1. the bacteriocin as-48 cyclic polypeptide (bacteriocin as-48) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.20.225.10. 1 hit.
InterProiIPR009086. Bacteriocin_AS48.
IPR020038. Bacteriocin_uberolysin_fam.
[Graphical view]
PfamiPF09221. Bacteriocin_IId. 1 hit.
[Graphical view]
SUPFAMiSSF47869. SSF47869. 1 hit.
TIGRFAMsiTIGR03651. circ_ocin_uber. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: PROTEIN SEQUENCE.
  2. "Determination of the gene sequence and the molecular structure of the enterococcal peptide antibiotic AS-48."
    Martinez-Bueno M., Maqueda M., Galvez A., Samyn B., Van Beeumen J., Coyette J., Valdivia E.
    J. Bacteriol. 176:6334-6339(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: S-48Imported.
    Plasmid: BglII
  3. "Analysis of the gene cluster involved in production and immunity of the peptide antibiotic AS-48 in Enterococcus faecalis."
    Martinez-Bueno M., Valdivia E., Galvez A., Coyette J., Maqueda M.
    Mol. Microbiol. 27:347-358(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: S-48Imported.
  4. "Bacteriocin AS-48, a microbial cyclic polypeptide structurally and functionally related to mammalian NK-lysin."
    Gonzalez C., Langdon G.M., Bruix M., Galvez A., Valdivia E., Maqueda M., Rico M.
    Proc. Natl. Acad. Sci. U.S.A. 97:11221-11226(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 36-105.
  5. "Structure of bacteriocin AS-48: from soluble state to membrane bound state."
    Sanchez-Barrena M.J., Martinez-Ripoll M., Galvez A., Valdivia E., Maqueda M., Cruz V., Albert A.
    J. Mol. Biol. 334:541-549(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.46 ANGSTROMS) OF 36-105.
  6. "Characterization and Application of Enterocin RM6, a Bacteriocin from Enterococcus faecalis."
    Huang E., Zhang L., Chung Y.K., Zheng Z., Yousef A.E.
    Biomed. Res. Int. 2013:206917-206917(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: OSY-RM6Imported.
  7. "Analysis of the Promoters Involved in Enterocin AS-48 Expression."
    Cebrian R., Rodriguez-Ruano S., Martinez-Bueno M., Valdivia E., Maqueda M., Montalban-Lopez M.
    PLoS ONE 9:E90603-E90603(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Plasmid: pTLR
  8. "The bacteriocin AS-48 requires dimer dissociation followed by hydrophobic interactions with the membrane for antibacterial activity."
    Cebrian R., Martinez-Bueno M., Valdivia E., Albert A., Maqueda M., Sanchez-Barrena M.J.
    J. Struct. Biol. 190:162-172(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) OF 36-105.
  9. "Bacteriocin production augments niche competition by enterococci in the mammalian gastrointestinal tract."
    Kommineni S., Bretl D.J., Lam V., Chakraborty R., Hayward M., Simpson P., Cao Y., Bousounis P., Kristich C.J., Salzman N.H.
    Nature 526:719-722(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: CK135Imported.
    Plasmid: pPD1

Entry informationi

Entry nameiQ47765_ENTFL
AccessioniPrimary (citable) accession number: Q47765
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, PlasmidImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.