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Q47758 (DDL_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:EF_0843
OrganismEnterococcus faecalis (strain ATCC 700802 / V583) [Reference proteome] [HAMAP]
Taxonomic identifier226185 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177821

Regions

Domain136 – 341206ATP-grasp
Nucleotide binding169 – 22456ATP By similarity

Sites

Metal binding2951Magnesium or manganese 1 By similarity
Metal binding3081Magnesium or manganese 1 By similarity
Metal binding3081Magnesium or manganese 2 By similarity
Metal binding3101Magnesium or manganese 2 By similarity

Experimental info

Sequence conflict1271A → V in AAC43218. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q47758 [UniParc].

Last modified April 23, 2003. Version 2.
Checksum: 4A690F4E4FEDBF75

FASTA34839,340
        10         20         30         40         50         60 
MKIILLYGGR SEEHDVSVLS AYSVLNAIYY KYYQVQLVFI SKDGQWVKGP LLSERPQNKE 

        70         80         90        100        110        120 
VLHLTWAQTP EETGEFSGKR ISPSEIYEEE AIVFPVLHGP NGEDGTIQGF METINMPYVG 

       130        140        150        160        170        180 
AGVLASANAM DKIMTKYLLQ TVGIPQVPFV PVLRSDWKGN PKEVFEKCEG SLIYPVFVKP 

       190        200        210        220        230        240 
ANMGSSVGIS KVENREELQE ALEEAFRYDA RAIVEQGIEA REIEVAILGN EDVRTTLPGE 

       250        260        270        280        290        300 
VVKDVAFYDY DAKYINNTIE MQIPAHVPEE VAHQAQEYAK KAYIMLDGSG LSRCDFFLTS 

       310        320        330        340 
KNELFLNELN TMPGFTDFSM YPLLWENMGL KYSDLIEELI QLALNRFK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the vanB and ddl genes encoding D-alanine:D-lactate and D-alanine:D-alanine ligases in vancomycin-resistant Enterococcus faecalis V583."
Evers S., Reynolds P.E., Courvalin P.
Gene 140:97-102(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 700802 / V583.
[2]"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis."
Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. expand/collapse author list , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.
Science 299:2071-2074(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700802 / V583.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00457 Genomic DNA. Translation: AAC43218.2.
AE016830 Genomic DNA. Translation: AAO80655.1.
RefSeqNP_814585.1. NC_004668.1.

3D structure databases

ProteinModelPortalQ47758.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING226185.EF0843.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO80655; AAO80655; EF_0843.
GeneID1199736.
KEGGefa:EF0843.
PATRIC21852066. VBIEntFae7065_0782.

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycEFAE226185:GHI1-824-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_ENTFA
AccessionPrimary (citable) accession number: Q47758
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 23, 2003
Last modified: February 19, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways