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Protein

Adenosylhomocysteinase

Gene

ahcY

Organism
Cupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.UniRule annotation

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.UniRule annotation

Cofactori

NAD+UniRule annotationNote: Binds 1 NAD+ per subunit.UniRule annotation

Pathwayi: L-homocysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylhomocysteinase (ahcY)
This subpathway is part of the pathway L-homocysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61SubstrateUniRule annotation1
Binding sitei136SubstrateUniRule annotation1
Binding sitei196SubstrateUniRule annotation1
Binding sitei226SubstrateUniRule annotation1
Binding sitei230SubstrateUniRule annotation1
Binding sitei231NADUniRule annotation1
Binding sitei283NADUniRule annotation1
Binding sitei318NADUniRule annotation1
Binding sitei384NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 199NADUniRule annotation3
Nucleotide bindingi260 – 265NADUniRule annotation6
Nucleotide bindingi339 – 341NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processOne-carbon metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
AdenosylhomocysteinaseUniRule annotation (EC:3.3.1.1UniRule annotation)
Alternative name(s):
S-adenosyl-L-homocysteine hydrolaseUniRule annotation
Short name:
AdoHcyaseUniRule annotation
Gene namesi
Name:ahcYUniRule annotation
Ordered Locus Names:Reut_A0213
OrganismiCupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000002697 Componenti: Chromosome 1

Subcellular locationi

Q476T8:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001292981 – 472AdenosylhomocysteinaseAdd BLAST472

Interactioni

Protein-protein interaction databases

STRINGi264198.Reut_A0213.

Structurei

3D structure databases

ProteinModelPortaliQ476T8.
SMRiQ476T8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3E. Bacteria.
COG0499. LUCA.
HOGENOMiHOG000227986.
KOiK01251.
OMAiTGNRDII.
OrthoDBiPOG091H00W2.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiView protein in InterPro
IPR034373. Adenosylhomocysteinase.
IPR000043. Adenosylhomocysteinase-like.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR036291. NAD(P)-bd_dom_sf.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiView protein in Pfam
PF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiView protein in SMART
SM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiView protein in PROSITE
PS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q476T8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAVTDLKQD YLVADISLAG WGRKEIAIAE TEMPGLMAIR DEFAAAQPLK
60 70 80 90 100
GARIAGSLHM TIQTAVLIET LKALGADVRW ASCNIFSTQD HAAAAIAAGG
110 120 130 140 150
TPVFAFKGES LKEYWDFTHR IFDWVDGGTP NMILDDGGDA TLLLHLGAKA
160 170 180 190 200
EKDASLIANP GSEEETFLFA AIKEKLAKDP SWYSRNLAAI RGVTEETTTG
210 220 230 240 250
VHRLYQMAQK GDLKFPAINV NDSVTKSKFD NLYGCRESLV DGIKRATDVM
260 270 280 290 300
IAGKIAIVAG YGDVGKGSAQ ALRALSAQVW VTEIDPICAL QAAMEGYRVV
310 320 330 340 350
TMDYAAEHGD IFVTCTGNYH VITHDHMARM KDQAIVCNIG HFDNEIDIAS
360 370 380 390 400
IEKYEWDEIK PQVDHVKFPD GKKIIILAKG RLVNLGCATG HPSYVMSSSF
410 420 430 440 450
ANQTIAQIEL WQERDSGKYP VGVYTLPKHL DEKVARLQLR KLNAQLTELT
460 470
EQQAAYIGVK KEGPYKADHY RY
Length:472
Mass (Da):51,843
Last modified:September 13, 2005 - v1
Checksum:iF8F0F670CF678BBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA. Translation: AAZ59595.1.
RefSeqiWP_011296403.1. NC_007347.1.

Genome annotation databases

EnsemblBacteriaiAAZ59595; AAZ59595; Reut_A0213.
KEGGireu:Reut_A0213.

Similar proteinsi

Entry informationi

Entry nameiSAHH_CUPNJ
AccessioniPrimary (citable) accession number: Q476T8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 13, 2005
Last modified: October 25, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families