Q476F8 (ISPE_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Reut_A0343

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235123
Regions
	Nucleotide binding	107 – 117	11	ATP Potential
Sites
	Active site	24	1	By similarity
	Active site	149	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q476F8 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: D6E5BEAB235D1C8A	FASTA	296	32,131
	10 20 30 40 50 60 MTAALTAAPT LPPPELRDCP APAKLNLFLH VTGRRPDGYH MLQTVFQLVD WCDTLHFRRR 70 80 90 100 110 120 DDGVVTRTTD IPGVPAETDL IVRAARALQQ ATGTRFGADI AIDKILPMGG GIGGGSSDAA 130 140 150 160 170 180 TTLLALNHLW GLGLPRAELM RIGLALGADV PVFVFGENAF AEGIGEELTA VDLPDSWFVI 190 200 210 220 230 240 VHPKQHVPTA EIFSDECLTR DTPLSIIAVF AACTNKFSFG RNDLEAIATA KFGEVARALE 250 260 270 280 290 WLKQFNQHAR MTGSGACVFA RFPDEQKAQQ VLKRLPPEWD GRCVRSLARH PLAAFA

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ59725.1.
RefSeq	YP_294569.1. NC_007347.1.
3D structure databases
ProteinModelPortal	Q476F8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q476F8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3609584.
GenomeReviews	Gene locus Reut_A0343 in contig CP000090_GR.
KEGG	reu:Reut_A0343.
NMPDR	fig|264198.3.peg.855.
PATRIC	20226133. VBIRalEut24049_0943.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	LNLFLHV.
ProtClustDB	PRK00343.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_A0343-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CUPPJ

Accession

Primary (citable) accession number: Q476F8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program