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Protein

Temperature-sensitive hemagglutinin tsh autotransporter

Gene

tsh

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the development of lesions and deposition of fibrin in the avian air sacs. It can act both as an adhesin and as a serine protease. Agglutinates erythrocytes while in contact with the extracellular surface of the bacterial cells. Can adhere to purified hemoglobin and bind with great efficiency to extracellular matrix proteins. Cleaves casein and exhibits mucinolytic activity.4 Publications

Temperature dependencei

Optimum temperature is 26 degrees Celsius for hemagglutination activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei125 – 1251Charge relay systemPROSITE-ProRule annotation
Active sitei153 – 1531Charge relay systemPROSITE-ProRule annotation
Active sitei259 – 2591Charge relay system

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Virulence

Protein family/group databases

MEROPSiS06.003.
TCDBi1.B.12.4.2. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Temperature-sensitive hemagglutinin tsh autotransporter (EC:3.4.21.-)
Alternative name(s):
Autotransporter tsh
Cleaved into the following 2 chains:
Gene namesi
Name:tsh
Encoded oniPlasmid pAPEC-13 Publications
Plasmid pAPEC-O2-ColV1 Publication
Plasmid pYA34321 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

Temperature-sensitive hemagglutinin tsh translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi259 – 2591S → A: Loss of proteolytic activity. No effect on hemagglutination activity. 2 Publications
Mutagenesisi259 – 2591S → T: No effect either on extracellular secretion or on hemagglutination activity. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 52521 PublicationAdd
BLAST
Chaini53 – 13771325Temperature-sensitive hemagglutinin tsh autotransporterPRO_0000387610Add
BLAST
Chaini53 – 11001048Temperature-sensitive hemagglutinin tshPRO_0000026982Add
BLAST
Chaini1101 – 1377277Temperature-sensitive hemagglutinin tsh translocatorPRO_0000026983Add
BLAST

Post-translational modificationi

The C-terminus is blocked.Curated
Cleaved to release the mature protein from the outer membrane.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1100 – 11012Cleavage

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiQ47692.

Structurei

3D structure databases

ProteinModelPortaliQ47692.
SMRiQ47692. Positions 1101-1377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 302250Peptidase S6PROSITE-ProRule annotationAdd
BLAST
Domaini1111 – 1377267AutotransporterPROSITE-ProRule annotationAdd
BLAST

Domaini

Adhesive and proteolytic functions of tsh lie in different domains of the protein.
The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation
Contains 1 peptidase S6 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

KOiK12684.

Family and domain databases

Gene3Di2.160.20.20. 3 hits.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR009003. Peptidase_S1_PA.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF50494. SSF50494. 2 hits.
SSF51126. SSF51126. 3 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q47692-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRIYSLRYS AVARGFIAVS EFARKCVHKS VRRLCFPVLL LIPVLFSAGS
60 70 80 90 100
LAGTVNNELG YQLFRDFAEN KGMFRPGATN IAIYNKQGEF VGTLDKAAMP
110 120 130 140 150
DFSAVDSEIG VATLINPQYI ASVKHNGGYT NVSFGDGENR YNIVDRNNAP
160 170 180 190 200
SLDFHAPRLD KLVTEVAPTA VTAQGAVAGA YLDKERYPVF YRLGSGTQYI
210 220 230 240 250
KDSNGQLTQM GGAYSWLTGG TVGSLSSYQN GEMISTSSGL VFDYKLNGAM
260 270 280 290 300
PIYGEAGDSG SPLFAFDTVQ NKWVLVGVLT AGNGAGGRGN NWAVIPLDFI
310 320 330 340 350
GQKFNEDNDA PVTFRTSEGG ALEWSFNSST GAGALTQGTT TYAMHGQQGN
360 370 380 390 400
DLNAGKNLIF QGQNGQINLK DSVSQGAGSL TFRDNYTVTT SNGSTWTGAG
410 420 430 440 450
IVVDNGVSVN WQVNGVKGDN LHKIGEGTLT VQGTGINEGG LKVGDGKVVL
460 470 480 490 500
NQQADNKGQV QAFSSVNIAS GRPTVVLTDE RQVNPDTVSW GYRGGTLDVN
510 520 530 540 550
GNSLTFHQLK AADYGAVLAN NVDKRATITL DYALRADKVA LNGWSESGKG
560 570 580 590 600
TAGNLYKYNN PYTNTTDYFI LKQSTYGYFP TDQSSNATWE FVGHSQGDAQ
610 620 630 640 650
KLVADRFNTA GYLFHGQLKG NLNVDNRLPE GVTGALVMDG AADISGTFTQ
660 670 680 690 700
ENGRLTLQGH PVIHAYNTQS VADKLAASGD HSVLTQPTSF SQEDWENRSF
710 720 730 740 750
TFDRLSLKNT DFGLGRNATL NTTIQADNSS VTLGDSRVFI DKNDGQGTAF
760 770 780 790 800
TLEEGTSVAT KDADKSVFNG TVNLDNQSVL NINDIFNGGI QANNSTVNIS
810 820 830 840 850
SDSAVLGNST LTSTALNLNK GANALASQSF VSDGPVNISD AALSLNSRPD
860 870 880 890 900
EVSHTLLPVY DYAGSWNLKG DDARLNVGPY SMLSGNINVQ DKGTVTLGGE
910 920 930 940 950
GELSPDLTLQ NQMLYSLFNG YRNIWSGSLN APDATVSMTD TQWSMNGNST
960 970 980 990 1000
AGNMKLNRTI VGFNGGTSPF TTLTTDNLDA VQSAFVMRTD LNKADKLVIN
1010 1020 1030 1040 1050
KSATGHDNSI WVNFLKKPSN KDTLDIPLVS APEATADNLF RASTRVVGFS
1060 1070 1080 1090 1100
DVTPILSVRK EDGKKEWVLD GYQVARNDGQ GKAAATFMHI SYNNFITEVN
1110 1120 1130 1140 1150
NLNKRMGDLR DINGEAGTWV RLLNGSGSAD GGFTDHYTLL QMGADRKHEL
1160 1170 1180 1190 1200
GSMDLFTGVM ATYTDTDASA DLYSGKTKSW GGGFYASGLF RSGAYFDVIA
1210 1220 1230 1240 1250
KYIHNENKYD LNFAGAGKQN FRSHSLYAGA EVGYRYHLTD TTFVEPQAEL
1260 1270 1280 1290 1300
VWGRLQGQTF NWNDSGMDVS MRRNSVNPLV GRTGVVSGKT FSGKDWSLTA
1310 1320 1330 1340 1350
RAGLHYEFDL TDSADVHLKD AAGEHQINGR KDSRMLYGVG LNARFGDNTR
1360 1370
LGLEVERSAF GKYNTDDAIN ANIRYSF
Length:1,377
Mass (Da):148,227
Last modified:November 1, 1996 - v1
Checksum:i237423644D9AE012
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti175 – 1751G → W in strain: APEC13.
Natural varianti183 – 1831D → G in strain: APEC13.
Natural varianti209 – 2091Q → K in strain: A2363 and APEC13.
Natural varianti326 – 3261F → S in strain: A2363.
Natural varianti422 – 4221H → Y in strain: A2363.
Natural varianti634 – 6341G → S in strain: A2363.
Natural varianti842 – 8421A → T in strain: A2363.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218073 Genomic DNA. Translation: AAA24698.1.
AY545598 Genomic DNA. Translation: AAT35241.2.
AY280856 Genomic DNA. Translation: AAP33781.1.
PIRiI54632.
RefSeqiWP_001080137.1. NZ_LRWY01000004.1.
WP_011402691.1. NC_007675.1.
YP_444132.1. NC_007675.1.

Genome annotation databases

GeneIDi3853553.
KEGGipg:3853553.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218073 Genomic DNA. Translation: AAA24698.1.
AY545598 Genomic DNA. Translation: AAT35241.2.
AY280856 Genomic DNA. Translation: AAP33781.1.
PIRiI54632.
RefSeqiWP_001080137.1. NZ_LRWY01000004.1.
WP_011402691.1. NC_007675.1.
YP_444132.1. NC_007675.1.

3D structure databases

ProteinModelPortaliQ47692.
SMRiQ47692. Positions 1101-1377.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS06.003.
TCDBi1.B.12.4.2. the autotransporter-1 (at-1) family.

Proteomic databases

PRIDEiQ47692.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3853553.
KEGGipg:3853553.

Phylogenomic databases

KOiK12684.

Family and domain databases

Gene3Di2.160.20.20. 3 hits.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR009003. Peptidase_S1_PA.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF50494. SSF50494. 2 hits.
SSF51126. SSF51126. 3 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSH_ECOLX
AccessioniPrimary (citable) accession number: Q47692
Secondary accession number(s): Q6QE02, Q83WR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.