Reviewed, UniProtKB/Swiss-Prot Q47690 (MMUM_ECOLI)

Names and origin

Protein names

Recommended name:
    Homocysteine S-methyltransferase
    EC=2.1.1.10
Alternative name(s):
    S-methylmethionine:homocysteine methyltransferase

Gene names

Name:	mmuM
Synonyms:	yagD
Ordered Locus Names:	b0261, JW0253

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes methyl transfer from S-methylmethionine or S-adenosylmethionine (less efficient) to homocysteine, selenohomocysteine and less efficiently selenocysteine. Ref.5
Catalytic activity	S-adenosyl-L-methionine + L-homocysteine = S-adenosyl-L-homocysteine + L-methionine.
Cofactor	Zinc Potential.
Subunit structure	Monomer.
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Homocysteine S-methyltransferase		PRO_0000114610
Regions
	Domain	1 – 310	310	Hcy-binding
Sites
	Metal binding	229	1	Zinc Potential
	Metal binding	295	1	Zinc Potential
	Metal binding	296	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q47690-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 8381CFF475E5FB7A	FASTA	310	33,423
	10 20 30 40 50 60 MSQNNPLRAL LDKQDILLLD GAMATELEAR GCNLADSLWS AKVLVENPEL IREVHLDYYR 70 80 90 100 110 120 AGAQCAITAS YQATPAGFAA RGLDEAQSKA LIGKSVELAR KAREAYLAEN PQAGTLLVAG 130 140 150 160 170 180 SVGPYGAYLA DGSEYRGDYH CSVEAFQAFH RPRVEALLDA GADLLACETL PNFSEIEALA 190 200 210 220 230 240 ELLTAYPRAR AWFSFTLRDS EHLSDGTPLR DVVALLAGYP QVVALGINCI ALENTTAALQ 250 260 270 280 290 300 HLHGLTVLPL VVYPNSGEHY DAVSKTWHHH GEHCAQLADY LPQWQAAGAR LIGGCCRTTP 310 ADIAALKARS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 123; 130; 142 AND 159. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"S-methylmethionine metabolism in Escherichia coli." Thanbichler M., Neuhierl B., Boeck A. J. Bacteriol. 181:662-665(1999) [PubMed: 9882684] [Abstract] Cited for: FUNCTION.
[6]	"A family of S-methylmethionine-dependent thiol/selenol methyltransferases. Role in selenium tolerance and evolutionary relation." Neuhierl B., Thanbichler M., Lottspeich F., Boeck A. J. Biol. Chem. 274:5407-5414(1999) [PubMed: 10026151] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / JM109 / ATCC 53323.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U70214 Genomic DNA. Translation: AAB08682.1. U00096 Genomic DNA. Translation: AAC73364.1. AP009048 Genomic DNA. Translation: BAA77929.2.
PIR	E64751.
RefSeq	AP_000914.1. NP_414795.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	946143.
GenomeReviews	Gene locus JW0253 in contig AP009048_GR. Gene locus b0261 in contig U00096_GR.
KEGG	ecj:JW0253. eco:b0261.
Organism-specific databases
EcoGene	EG13343. mmuM.
CMR	Search...
Phylogenomic databases
HOGENOM	Q47690.
OMA	Q47690. NSYALVP.
Enzyme and pathway databases
BioCyc	EcoCyc:MMUM-MON. MetaCyc:MMUM-MON.
Family and domain databases
InterPro	IPR003726. S_MeTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.330. S_methyl_trans. 1 hit.
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MMUM_ECOLI

Accession

Primary (citable) accession number: Q47690
Secondary accession number(s): P77226

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)