Reviewed, UniProtKB/Swiss-Prot Q47679 (YAFV_ECOLI)

Names and origin

Protein names

Recommended name:
    UPF0012 hydrolase yafV
    EC=3.5.-.-

Gene names

Name:	yafV
Ordered Locus Names:	b0219, JW5019

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Sequence similarities

Belongs to the UPF0012 family. Contains 1 CN hydrolase domain.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	UPF0012 hydrolase yafV		PRO_0000213257
Regions
	Domain	4 – 256	253	CN hydrolase
Sites
	Active site	42	1	Proton acceptor Potential
	Active site	107	1	By similarity
	Active site	141	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47679-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7C2381648065660C	FASTA	256	28,925
	10 20 30 40 50 60 MPGLKITLLQ QPLVWMDGPA NLRHFDRQLE GITGRDVIVL PEMFTSGFAM EAAASSLAQD 70 80 90 100 110 120 DVVNWMTAKA QQCNALIAGS VALQTESGSV NRFLLVEPGG TVHFYDKRHL FRMADEHLHY 130 140 150 160 170 180 KAGNARVIVE WRGWRILPLV CYDLRFPVWS RNLNDYDLAL YVANWPAPRS LHWQALLTAR 190 200 210 220 230 240 AIENQAYVAG CNRVGSDGNG CHYRGDSRVI NPQGEIIATA DAHQATRIDA ELSMAALREY 250 REKFPAWQDA DEFRLW

References

[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U70214 Genomic DNA. Translation: AAB08641.1. U00096 Genomic DNA. Translation: AAC73323.1. AP009048 Genomic DNA. Translation: BAA77889.2.
PIR	D64746.
RefSeq	AP_000874.1. NP_414754.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q47679.
Genome annotation databases
GeneID	946585.
GenomeReviews	Gene locus JW5019 in contig AP009048_GR. Gene locus b0219 in contig U00096_GR.
KEGG	ecj:JW5019. eco:b0219.
Organism-specific databases
EchoBASE	EB3118.
EcoGene	EG13334. yafV.
CMR	Search...
Phylogenomic databases
HOGENOM	Q47679.
OMA	WHDREAN
Enzyme and pathway databases
BioCyc	EcoCyc:G6103-MON. ECOL168927:B0219-MON.
Gene expression databases
Genevestigator	Q47679.
Family and domain databases
InterPro	IPR003010. Ntlse/CNhydtse. IPR001110. UPF0012_CS. [Graphical view]
Gene3D	G3DSA:3.60.110.10. Ntlse/CNhydtse. 1 hit.
Pfam	PF00795. CN_hydrolase. 1 hit. [Graphical view]
PROSITE	PS50263. CN_HYDROLASE. 1 hit. PS01227. UPF0012. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAFV_ECOLI

Accession

Primary (citable) accession number: Q47679
Secondary accession number(s): Q9R2E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)