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Protein

Omega-amidase YafV

Gene

yafV

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Hydrolyzes alpha-ketoglutaramate (a-KGM) to alpha-ketoglutarate (alpha-KG) and ammonia, has weak activity on L-glutamine, almost no activity on deaminated glutathione (dGSH) and none on glutathione (By similarity). May function as a metabolite repair enzyme (By similarity).By similarity

Catalytic activityi

A monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei42Proton acceptorPROSITE-ProRule annotation1
Active sitei107Proton donorPROSITE-ProRule annotation1
Active sitei141NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G6103-MONOMER
MetaCyc:G6103-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-amidase YafV (EC:3.5.1.3By similarity)
Gene namesi
Name:yafV
Ordered Locus Names:b0219, JW5019
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13334 yafV

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002132571 – 256Omega-amidase YafVAdd BLAST256

Proteomic databases

PaxDbiQ47679
PRIDEiQ47679

Interactioni

Protein-protein interaction databases

BioGridi4261824, 18 interactors
DIPiDIP-11225N
STRINGi316385.ECDH10B_0201

Structurei

3D structure databases

ProteinModelPortaliQ47679
SMRiQ47679
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 234CN hydrolasePROSITE-ProRule annotationAdd BLAST231

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CZ7 Bacteria
COG0388 LUCA
HOGENOMiHOG000222700
InParanoidiQ47679
KOiK13566
OMAiPEMFSTG
PhylomeDBiQ47679

Family and domain databases

Gene3Di3.60.110.10, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR001110 UPF0012_CS
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit
PS01227 UPF0012, 1 hit

Sequencei

Sequence statusi: Complete.

Q47679-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGLKITLLQ QPLVWMDGPA NLRHFDRQLE GITGRDVIVL PEMFTSGFAM
60 70 80 90 100
EAAASSLAQD DVVNWMTAKA QQCNALIAGS VALQTESGSV NRFLLVEPGG
110 120 130 140 150
TVHFYDKRHL FRMADEHLHY KAGNARVIVE WRGWRILPLV CYDLRFPVWS
160 170 180 190 200
RNLNDYDLAL YVANWPAPRS LHWQALLTAR AIENQAYVAG CNRVGSDGNG
210 220 230 240 250
CHYRGDSRVI NPQGEIIATA DAHQATRIDA ELSMAALREY REKFPAWQDA

DEFRLW
Length:256
Mass (Da):28,925
Last modified:November 1, 1996 - v1
Checksum:i7C2381648065660C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA Translation: AAB08641.1
U00096 Genomic DNA Translation: AAC73323.1
AP009048 Genomic DNA Translation: BAA77889.2
PIRiD64746
RefSeqiNP_414754.1, NC_000913.3
WP_001118036.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73323; AAC73323; b0219
BAA77889; BAA77889; BAA77889
GeneIDi946585
KEGGiecj:JW5019
eco:b0219
PATRICifig|1411691.4.peg.2064

Similar proteinsi

Entry informationi

Entry nameiYAFV_ECOLI
AccessioniPrimary (citable) accession number: Q47679
Secondary accession number(s): Q9R2E1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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