Q475B2 (ILVD1_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 1
Short name=DAD 1
EC=4.2.1.9

Gene names

Name:	ilvD1
Ordered Locus Names:	Reut_A0639

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Dihydroxy-acid dehydratase 1 HAMAP MF_00012		PRO_0000225415
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q475B2 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 0DA8594E77A62D00	FASTA	557	59,279
	10 20 30 40 50 60 MAFNKRSRNI TQGVARSPNR SMYYALGYQK EDFDKPMVGI ANGHSTITPC NAGLQRLADA 70 80 90 100 110 120 AIDAVKASDA NPQVFGTPTI SDGMSMGTEG MKYSLISREV IADCIETAAQ GQWMDGVVVI 130 140 150 160 170 180 GGCDKNMPGG MIALARTNVP GIYVYGGTIK PGNWKGKDLT IVSSFEAVGE FTAGRMSEED 190 200 210 220 230 240 FEGVERNACP STGSCGGMYT ANTMSSSFEA LGMSLLYSST MANPDQEKVD SAAESARVLV 250 260 270 280 290 300 EAIKKDIKPR DIITRKSIEN AVALIMATGG STNAVLHYLA IAHSAEVEWT IDDFERIRRK 310 320 330 340 350 360 VPVICNLKPS GQYVATDLHK AGGIPQVMKI LLKAGLLHGD CLTITGRTLA EELEHVPDQP 370 380 390 400 410 420 RADQDVIMPI EKALYAEGHL AILKGNLAEE GAVAKITGLK NPVITGPARV FEDEQSAMEA 430 440 450 460 470 480 ILADKINAGD VLVLRYLGPK GGPGMPEMLA PTSAIIGKGL GESVGFITDG RFSGGTWGMV 490 500 510 520 530 540 VGHVAPEAYV GGNIALVREG DSITIDAHKL VLQVNVSDEE LARRRAEWKQ PVARYTRGVL 550 AKFARLASTA SKGAVTD

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ60021.1.
RefSeq	YP_294865.1. NC_007347.1.
3D structure databases
ProteinModelPortal	Q475B2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q475B2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3610628.
GenomeReviews	Gene locus Reut_A0639 in contig CP000090_GR.
KEGG	reu:Reut_A0639.
NMPDR	fig|264198.3.peg.1128.
PATRIC	20226733. VBIRalEut24049_1242.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	NMPGAMI.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_A0639-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD1_CUPPJ

Accession

Primary (citable) accession number: Q475B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program