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Protein

Microcin C7

Gene

mccA

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Antibacterial peptide, active against enterobacteria including species of Klebsiella, Salmonella, Shigella, Yersinia and Proteus, and strains of E.coli. Inhibits protein translation by blocking aspartyl-tRNA synthetase function and inhibiting production of aminoacetylated tRNA-Asp.5 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial

Names & Taxonomyi

Protein namesi
Recommended name:
Microcin C7
Short name:
MccC7
Alternative name(s):
Microcin C51
Short name:
McC
Short name:
MccC51
Short name:
Microcin C
Gene namesi
Name:mccAImported
Encoded oniPlasmid pMccC7Imported
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 77Microcin C72 PublicationsPRO_0000341532

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-formylmethionine2 Publications
Modified residuei7 – 71Aspartic acid 1-[(3-aminopropyl)(5'-adenosyl)phosphono]amide2 Publications

Post-translational modificationi

The peptide moiety allows entry into the bacterial cell, where it undergoes proteolytic cleavage to release the aspartyl adenylate analog, which is responsible for aspartyl-tRNA synthetase inhibition. Can be processed by the non-specific oligopeptidases PepA, PepB and PepN.4 Publications

Keywords - PTMi

Formylation, Phosphoprotein

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
mccBQ475068EBI-7035319,EBI-7035293

Protein-protein interaction databases

IntActiQ47505. 1 interaction.
MINTiMINT-7265788.

Structurei

Secondary structure

1
7
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H5RX-ray2.10E/F/G/H1-7[»]
3H9GX-ray2.20E/F/G/H1-7[»]
3H9JX-ray2.30E/F/G/H1-7[»]
3H9QX-ray2.63E/F/G/H1-7[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ47505.

Sequencei

Sequence statusi: Complete.

Length:7
Mass (Da):763
Last modified:November 1, 1996 - v1
Checksum:i644DD44861B406F0
GO

Mass spectrometryi

Molecular mass is 1177±1 Da from positions 1 - 7. Determined by ESI. 2 Publications
Molecular mass is 1177.5 Da from positions 1 - 7. Determined by PD. 2 Publications

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57583 Genomic DNA. Translation: CAA40808.1.
PIRiS45311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57583 Genomic DNA. Translation: CAA40808.1.
PIRiS45311.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H5RX-ray2.10E/F/G/H1-7[»]
3H9GX-ray2.20E/F/G/H1-7[»]
3H9JX-ray2.30E/F/G/H1-7[»]
3H9QX-ray2.63E/F/G/H1-7[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ47505. 1 interaction.
MINTiMINT-7265788.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ47505.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiMCCC7_ECOLX
AccessioniPrimary (citable) accession number: Q47505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.