Q474U2 (Q474U2_CUPPJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroorotase HAMAP-Rule MF_00220
Short name=DHOase HAMAP-Rule MF_00220
EC=3.5.2.3 HAMAP-Rule MF_00220

Gene names

Name:	pyrC HAMAP-Rule MF_00220
Ordered Locus Names:	Reut_A0709 EMBL AAZ60091.1

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP] EMBL AAZ60091.1

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus pinatubonensis

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP-Rule MF_00220
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP-Rule MF_00220
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP-Rule MF_00220
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00220
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily. HAMAP-Rule MF_00220

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis HAMAP-Rule MF_00220
Ligand	Metal-binding HAMAP-Rule MF_00220 Zinc HAMAP-Rule MF_00220
Molecular function	Hydrolase HAMAP-Rule MF_00220 EMBL AAZ60091.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	324	1	Zinc 1 By similarity HAMAP-Rule MF_00220

Sequences

	Sequence		Length	Mass (Da)
	Q474U2 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 208196D0FED1E9C0	FASTA	448	47,783
	10 20 30 40 50 60 MPTSSQNIPN TPSDTTSQAM KIHIQGGRLI DPAANLDAQQ DLYIAAGKIV GVGSAPADFT 70 80 90 100 110 120 ANKTIDARGL IVCPGLVDLS ARLREPGYEY KATLESEVAA AAAGGVTSLL CPPDTDPVLD 130 140 150 160 170 180 EPGLVEMLKF RARTLNQTHV YPLGALTQGL KGEVLTEMNQ LTEAGCVGFS QAEAPVRNTQ 190 200 210 220 230 240 VLLRALQYAQ TFGFTVWLRP EDPFLGGGVA ASGAVASRLG LSGVSVIAET VRLHTIFELM 250 260 270 280 290 300 RASGARVHLC RLSTAAGLEL VRQAKREGLA VTCDVNIHHV SLTDMDIGYF NSQMRFSPPL 310 320 330 340 350 360 RSARDRDAIV AALADGTIDA LCSDHTPVDD DEKLLPFAEA TPGAIGLELL LPLTLRWAAE 370 380 390 400 410 420 HKVPLKTALA RITSEPARVA GLKAGTLASG AVADICVFDP KEVWKVDRQS IKSQGKNSPW 430 440 LGYEMEGRVR MTLVGGQVVY GNNNHNHH

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ60091.1.
RefSeq	YP_294935.1. NC_007347.1.
3D structure databases
ProteinModelPortal	Q474U2.
ModBase	Search...
Protein-protein interaction databases
STRING	264198.Reut_A0709.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ60091; AAZ60091; Reut_A0709.
GeneID	3609292.
KEGG	reu:Reut_A0709.
PATRIC	20226879. VBIRalEut24049_1315.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0044.
HOGENOM	HOG000219144.
KO	K01465.
OMA	SHHQPHE.
ProtClustDB	PRK07627.
Enzyme and pathway databases
BioCyc	CPIN264198:GIW3-713-MONOMER.
UniPathway	UPA00070; UER00117.
Family and domain databases
HAMAP	MF_00220_B. PyrC_type2_B.
InterPro	IPR004722. DHOase. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q474U2_CUPPJ

Accession

Primary (citable) accession number: Q474U2

Entry history

Integrated into UniProtKB/TrEMBL:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)