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Q474P5

- TRMB_CUPPJ

UniProt

Q474P5 - TRMB_CUPPJ

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851S-adenosyl-L-methionineUniRule annotation
Binding sitei110 – 1101S-adenosyl-L-methionineUniRule annotation
Binding sitei137 – 1371S-adenosyl-L-methionineUniRule annotation
Active sitei160 – 1601By similarity
Binding sitei160 – 1601S-adenosyl-L-methionineUniRule annotation
Binding sitei164 – 1641SubstrateUniRule annotation
Binding sitei196 – 1961SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCPIN264198:GIW3-762-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Reut_A0758
OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002697: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256tRNA (guanine-N(7)-)-methyltransferasePRO_0000229190Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264198.Reut_A0758.

Structurei

3D structure databases

ProteinModelPortaliQ474P5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 1716Interaction with RNAUniRule annotation
Regioni234 – 2374Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiHSTLEMA.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q474P5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHPQDASTEQ TPVDDDQVES SQPVHAPEDV AHPRRIRSFV RRAGRTSSGQ
60 70 80 90 100
QRAIDEVGPR LIVPYAPHPL DWEAAFGRKA PAILEIGFGM GETTAHIAQL
110 120 130 140 150
RPQDNFLGCE VHEPGVGALL KLADEREIGN IRIIQHDAVE VVAHMLTEDC
160 170 180 190 200
LDGVHIYFPD PWHKKRHNKR RLVQPPLVKL LASRLKPGGY IHCATDWEEY
210 220 230 240 250
AHQMVEVLAG EPALENASSA ADGFSERPEY RPITKFERRG VRLGHGVWDV

VFRKRG
Length:256
Mass (Da):28,734
Last modified:September 13, 2005 - v1
Checksum:iFE84986C69F21CAE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000090 Genomic DNA. Translation: AAZ60138.1.
RefSeqiWP_011296943.1. NC_007347.1.
YP_294982.1. NC_007347.1.

Genome annotation databases

EnsemblBacteriaiAAZ60138; AAZ60138; Reut_A0758.
GeneIDi3609918.
KEGGireu:Reut_A0758.
PATRICi20226977. VBIRalEut24049_1364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000090 Genomic DNA. Translation: AAZ60138.1 .
RefSeqi WP_011296943.1. NC_007347.1.
YP_294982.1. NC_007347.1.

3D structure databases

ProteinModelPortali Q474P5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264198.Reut_A0758.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ60138 ; AAZ60138 ; Reut_A0758 .
GeneIDi 3609918.
KEGGi reu:Reut_A0758.
PATRICi 20226977. VBIRalEut24049_1364.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
KOi K03439.
OMAi HSTLEMA.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci CPIN264198:GIW3-762-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia eutropha JMP134."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JMP134 / LMG 1197.

Entry informationi

Entry nameiTRMB_CUPPJ
AccessioniPrimary (citable) accession number: Q474P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3