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Q474P5

- TRMB_CUPPJ

UniProt

Q474P5 - TRMB_CUPPJ

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, Reut_A0758
Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851S-adenosyl-L-methionine By similarity
Binding sitei110 – 1101S-adenosyl-L-methionine By similarity
Binding sitei137 – 1371S-adenosyl-L-methionine By similarity
Active sitei160 – 1601 By similarity
Binding sitei160 – 1601S-adenosyl-L-methionine By similarity
Binding sitei164 – 1641Substrate By similarity
Binding sitei196 – 1961Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCPIN264198:GIW3-762-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:Reut_A0758
    OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
    Taxonomic identifieri264198 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000002697: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 256256tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_0000229190Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi264198.Reut_A0758.

    Structurei

    3D structure databases

    ProteinModelPortaliQ474P5.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 1716Interaction with RNA Reviewed prediction
    Regioni234 – 2374Substrate binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q474P5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHPQDASTEQ TPVDDDQVES SQPVHAPEDV AHPRRIRSFV RRAGRTSSGQ    50
    QRAIDEVGPR LIVPYAPHPL DWEAAFGRKA PAILEIGFGM GETTAHIAQL 100
    RPQDNFLGCE VHEPGVGALL KLADEREIGN IRIIQHDAVE VVAHMLTEDC 150
    LDGVHIYFPD PWHKKRHNKR RLVQPPLVKL LASRLKPGGY IHCATDWEEY 200
    AHQMVEVLAG EPALENASSA ADGFSERPEY RPITKFERRG VRLGHGVWDV 250
    VFRKRG 256
    Length:256
    Mass (Da):28,734
    Last modified:September 13, 2005 - v1
    Checksum:iFE84986C69F21CAE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000090 Genomic DNA. Translation: AAZ60138.1.
    RefSeqiYP_294982.1. NC_007347.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ60138; AAZ60138; Reut_A0758.
    GeneIDi3609918.
    KEGGireu:Reut_A0758.
    PATRICi20226977. VBIRalEut24049_1364.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000090 Genomic DNA. Translation: AAZ60138.1 .
    RefSeqi YP_294982.1. NC_007347.1.

    3D structure databases

    ProteinModelPortali Q474P5.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 264198.Reut_A0758.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ60138 ; AAZ60138 ; Reut_A0758 .
    GeneIDi 3609918.
    KEGGi reu:Reut_A0758.
    PATRICi 20226977. VBIRalEut24049_1364.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci CPIN264198:GIW3-762-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 1 of Ralstonia eutropha JMP134."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JMP134 / LMG 1197.

    Entry informationi

    Entry nameiTRMB_CUPPJ
    AccessioniPrimary (citable) accession number: Q474P5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: September 13, 2005
    Last modified: June 11, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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