Reviewed, UniProtKB/Swiss-Prot Q474L3 (GLYA1_RALEJ)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 1
      Short name=Serine methylase 1
      Short name=SHMT 1
    EC=2.1.2.1

Gene names

Name:	glyA1
Ordered Locus Names:	Reut_A0790

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Serine hydroxymethyltransferase 1 HAMAP MF_00051		PRO_0000235012
Amino acid modifications
	Modified residue	230	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q474L3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 9A72AE4E6B5BF7A3	FASTA	415	45,087
	10 20 30 40 50 60 MFERSRYTID QIDPEVFAAI QKENQRQEDH IELIASENYT SPAVMAAQGS QLTNKYAEGY 70 80 90 100 110 120 PGKRYYGGCE YVDIVEQLAI DRVKQLFGAE AANVQPNSGS QANQGVYFAV LKPGDTIMGM 130 140 150 160 170 180 SLAEGGHLTH GMALNMSGKW FNVVSYGLNA QEDIDYDALE KLAQEKKPKL IIAGASAFAL 190 200 210 220 230 240 RIDFERIGKV AKSIGAYFMV DMAHYAGLIA AGVYPNPVPH ADFVTTTTHK SLRGPRGGVI 250 260 270 280 290 300 LMKAEHEKAI NSSIFPGIQG GPLMHVIAGK AVAFKEALTP EFKAYQEQVV KNAAVLAETL 310 320 330 340 350 360 IARGLRIVSG RTESHVMLVD LRAKNITGKE AERILGEAHL TVNKNAIPND PEKPFVTSGI 370 380 390 400 410 RVGSPAMTTR GFKEEEARIV GNLIADVLDN PHDAGNIASV REQVSALTKR FPVYG

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ60170.1.
RefSeq	YP_295014.1.
3D structure databases
SMR	Q474L3. Positions 4-415.
ModBase	Search...
Genome annotation databases
GeneID	3609970.
GenomeReviews	Gene locus Reut_A0790 in contig CP000090_GR.
KEGG	reu:Reut_A0790.
NMPDR	fig|264198.3.peg.1304.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG301263.
OMA	FALRIDF.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_A0790-MONOMER.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA1_RALEJ

Accession

Primary (citable) accession number: Q474L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2005
Last modified:	January 19, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)