Q47474 (PMEB_DICD3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectinesterase B
Short name=PE B
EC=3.1.1.11

Alternative name(s):
Pectin methylesterase B

Gene names

Name:	pemB
Ordered Locus Names:	Dda3937_03435

Organism

Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) [Complete proteome] [HAMAP]

Taxonomic identifier

198628 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in the degradation of methylated oligogalacturonides present in the periplasm.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Cell outer membrane; Lipid-anchor.
Induction	By pectin.
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 433	412	Pectinesterase B		PRO_0000023499
Sites
	Active site	259	1	Proton donor By similarity
	Active site	292	1	Nucleophile By similarity
	Binding site	202	1	Substrate By similarity
	Binding site	236	1	Substrate By similarity
	Binding site	356	1	Substrate By similarity
	Binding site	358	1	Substrate By similarity
Amino acid modifications
	Lipidation	22	1	N-palmitoyl cysteine Ref.1
	Lipidation	22	1	S-diacylglycerol cysteine Probable
Experimental info
	Sequence conflict	245 – 258	14	DRVQL…LLSRQ → ESGATGKCPPAQPS in CAA59151. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q47474 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 73C2B9E8ED50281F	FASTA	433	47,190
	10 20 30 40 50 60 MSLTHYSGLA AAVSMSLILT ACGGQTPNSA RFQPVFPGTV SRPVLSAQEA GRFTPQHYFA 70 80 90 100 110 120 HGGEYAKPVA DGWTPTPIDT SRVTAAYVVG PRAGVAGATH TSIQQAVNAA LRQHPGQTRV 130 140 150 160 170 180 YIKLLPGTYT GTVYVPEGAP PLTLFGAGDR PEQVVVSLAL DSMMSPADYR ARVNPHGQYQ 190 200 210 220 230 240 PADPAWYMYN ACATKAGATI NTTCSAVMWS QSNDFQLKNL TVVNALLDTV DSGTHQAVAL 250 260 270 280 290 300 RTDGDRVQLE NVRLLSRQDT FFVNTSDRQN SYVTDHYSRA YIKDSYIEGD VDYVFGRATA 310 320 330 340 350 360 VFDRVRFHTV SSRGSKEAYV FAPDSIPSVK YGFLVINSQL TGDNGYRGAQ KAKLGRAWDQ 370 380 390 400 410 420 GAKQTGYLPG KTANGQLVIR DSTIDSSYDL ANPWGAAATT DRPFKGNISP QRDLDDIHFN 430 RLWEYNTQVL LHE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of pectin methylesterase B, an outer membrane lipoprotein of Erwinia chrysanthemi 3937." Shevchik V.E., Condemine G., Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. Mol. Microbiol. 19:455-466(1996) [PubMed: 8830237] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PALMITOYLATION. Strain: 3937.
[2]	"Complete genome sequence of Dickeya dadantii 3937." International Erwinia Consortium Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3937.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X84665 Genomic DNA. Translation: CAA59151.1. CP002038 Genomic DNA. Translation: ADM98093.1.
PIR	S70914.
RefSeq	YP_003882650.1. NC_014500.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9733317.
GenomeReviews	Gene locus Dda3937_03435 in contig CP002038_GR.
KEGG	ddd:Dda3937_03435.
PATRIC	42316098. VBIDicDad25310_1873.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01051.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00800. PECTINESTERASE_1. 1 hit. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMEB_DICD3

Accession

Primary (citable) accession number: Q47474
Secondary accession number(s): E0SL58

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 30, 2010
Last modified:	January 25, 2012
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program