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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Cupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi488MagnesiumUniRule annotation1
Metal bindingi494MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Reut_A0957
OrganismiCupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000002697 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003297951 – 728Polyribonucleotide nucleotidyltransferaseAdd BLAST728

Interactioni

Protein-protein interaction databases

STRINGi264198.Reut_A0957.

Structurei

3D structure databases

ProteinModelPortaliQ473U7.
SMRiQ473U7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini555 – 614KHUniRule annotationAdd BLAST60
Domaini624 – 692S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRYMHNYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q473U7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMFNKVVKQ FQWGQHTVRM ETGEIARQAG GAVIVDVEDT VVLATVVAAK
60 70 80 90 100
SPKPGQDFFP LTVDYIEKTY AAGKIPGGFF KREGRPSENE TLTSRLIDRP
110 120 130 140 150
LRPLFPEGFY NDVQVVVHVV SLNPEIPADI PALIGASAAL AVSGIPFNGP
160 170 180 190 200
VGAARIGYKD GQYLLNPTRS QMATSDLDLV VAGTERAVLM VESEANQLSE
210 220 230 240 250
DVMLGAVVYG HEQMQIAINA IHDLVREGGK PEWDWAPAPK NEALIAKVTE
260 270 280 290 300
VALPLLQEAY QLRQKSARSQ KLKEVSANVT AALAEAGVDA DKVEVGNIMF
310 320 330 340 350
DLEAKIVRGQ ILGGEPRIDG RDTRTVRPIE IRSSVLPRAH GSALFTRGET
360 370 380 390 400
QALVVATLGT KSDEQIIDAL AGEYRDRFML HYNMPPFATG ETGRVGSPKR
410 420 430 440 450
REIGHGRLAK RALIPVLPKD DEFAYTIRLV SEITESNGSS SMASVCGGCL
460 470 480 490 500
ALMDAGVPVK AHVAGVAMGL ILEGNKFAVL TDILGDEDHL GDMDFKVAGT
510 520 530 540 550
DNGITALQMD IKVQGITKEI MQVALAQAKE GRLHILGAMQ GAMGHARTEL
560 570 580 590 600
SAHAPRMITM KIHPDKIREV IGKGGSTIQA LTKETGTTID IQEDGTITIA
610 620 630 640 650
STSTDGMAEA KRRIEGITAE AEVGKIYNGT VLKLLDFGAI VNILPGKDGL
660 670 680 690 700
LHISEIVNER VKDIKDWLKE GQQLRVKLIQ ADEKGRLRLS LKAALAEEGG
710 720
SISPVNAGEA APAPAPAAAP ATPSDQQQ
Length:728
Mass (Da):77,990
Last modified:September 13, 2005 - v1
Checksum:i058A2E532EF2BC44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA. Translation: AAZ60336.1.
RefSeqiWP_011297140.1. NC_007347.1.

Genome annotation databases

EnsemblBacteriaiAAZ60336; AAZ60336; Reut_A0957.
KEGGireu:Reut_A0957.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPNP_CUPNJ
AccessioniPrimary (citable) accession number: Q473U7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 13, 2005
Last modified: June 7, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families