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Protein

Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE

Gene

arnE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the periplasmic side of the inner membrane.1 Publication

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • substrate-specific transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • lipid A biosynthetic process Source: UniProtKB-HAMAP
  • lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
  • response to iron(III) ion Source: EcoCyc
  • transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-2682.
ECOL316407:JW2252-MONOMER.
MetaCyc:MONOMER0-2682.
UniPathwayiUPA00030.

Protein family/group databases

TCDBi2.A.7.22.1. the drug/metabolite transporter (dmt) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE
Short name:
L-Ara4N-phosphoundecaprenol flippase subunit ArnE
Alternative name(s):
Undecaprenyl phosphate-aminoarabinose flippase subunit ArnE
Gene namesi
Name:arnE
Synonyms:pmrL, yfbW
Ordered Locus Names:b4544, JW2252
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14344. arnE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535CytoplasmicSequence analysisAdd
BLAST
Transmembranei36 – 5621HelicalSequence analysisAdd
BLAST
Topological domaini57 – 615PeriplasmicSequence analysis
Transmembranei62 – 8221HelicalSequence analysisAdd
BLAST
Topological domaini83 – 875CytoplasmicSequence analysis
Transmembranei88 – 10821HelicalSequence analysisAdd
BLAST
Topological domaini109 – 1113PeriplasmicSequence analysis

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene lack L-Ara4N-modified lipid A and are sensitive to polymyxin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 111111Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnEPRO_0000169184Add
BLAST

Proteomic databases

PaxDbiQ47377.

Interactioni

Subunit structurei

Heterodimer of ArnE and ArnF.1 Publication

Protein-protein interaction databases

BioGridi4260500. 209 interactions.
STRINGi511145.b4544.

Structurei

3D structure databases

ProteinModelPortaliQ47377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 10970EamAAdd
BLAST

Sequence similaritiesi

Belongs to the ArnE family.Curated
Contains 1 EamA domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41060GT. Bacteria.
COG0697. LUCA.
HOGENOMiHOG000261165.
InParanoidiQ47377.
KOiK12962.
OMAiLLTCAGQ.
OrthoDBiEOG6K13QJ.

Family and domain databases

HAMAPiMF_01869. Flippase_ArnE.
InterProiIPR000620. EamA_dom.
IPR022883. Flippase_ArnE.
[Graphical view]
PfamiPF00892. EamA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIWLTLVFAS LLSVAGQLCQ KQATCFVAIN KRRKHIVLWL GLALACLGLA
60 70 80 90 100
MVLWLLVLQN VPVGIAYPML SLNFVWVTLA AVKLWHEPVS PRHWCGVAFI
110
IGGIVILGST V
Length:111
Mass (Da):12,192
Last modified:April 27, 2001 - v2
Checksum:i7CFA06D75DA33D69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: ABD18693.1.
AP009048 Genomic DNA. Translation: BAE76675.1.
L35031 Genomic DNA. Translation: AAB04895.1.
RefSeqiWP_000638031.1. NZ_LN832404.1.
YP_588462.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiABD18693; ABD18693; b4544.
BAE76675; BAE76675; BAE76675.
GeneIDi1450282.
KEGGiecj:JW2252.
eco:b4544.
PATRICi32119881. VBIEscCol129921_2350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: ABD18693.1.
AP009048 Genomic DNA. Translation: BAE76675.1.
L35031 Genomic DNA. Translation: AAB04895.1.
RefSeqiWP_000638031.1. NZ_LN832404.1.
YP_588462.1. NC_000913.3.

3D structure databases

ProteinModelPortaliQ47377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260500. 209 interactions.
STRINGi511145.b4544.

Protein family/group databases

TCDBi2.A.7.22.1. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PaxDbiQ47377.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD18693; ABD18693; b4544.
BAE76675; BAE76675; BAE76675.
GeneIDi1450282.
KEGGiecj:JW2252.
eco:b4544.
PATRICi32119881. VBIEscCol129921_2350.

Organism-specific databases

EchoBASEiEB4090.
EcoGeneiEG14344. arnE.

Phylogenomic databases

eggNOGiENOG41060GT. Bacteria.
COG0697. LUCA.
HOGENOMiHOG000261165.
InParanoidiQ47377.
KOiK12962.
OMAiLLTCAGQ.
OrthoDBiEOG6K13QJ.

Enzyme and pathway databases

UniPathwayiUPA00030.
BioCyciEcoCyc:MONOMER0-2682.
ECOL316407:JW2252-MONOMER.
MetaCyc:MONOMER0-2682.

Miscellaneous databases

PROiQ47377.

Family and domain databases

HAMAPiMF_01869. Flippase_ArnE.
InterProiIPR000620. EamA_dom.
IPR022883. Flippase_ArnE.
[Graphical view]
PfamiPF00892. EamA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Menaquinone (vitamin K2) biosynthesis: localization and characterization of the menE gene from Escherichia coli."
    Sharma V., Hudspeth M.E.S., Meganathan R.
    Gene 168:43-48(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-111.
    Strain: K12.
  4. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli."
    Yan A., Guan Z., Raetz C.R.H.
    J. Biol. Chem. 282:36077-36089(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiARNE_ECOLI
AccessioniPrimary (citable) accession number: Q47377
Secondary accession number(s): Q2EES8, Q2MAN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: February 17, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.