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Protein

Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE

Gene

arnE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the periplasmic side of the inner membrane.1 Publication

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Biological processi

  • lipid A biosynthetic process Source: UniProtKB-KW
  • lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
  • response to iron(III) ion Source: EcoCyc

Keywordsi

Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-2682
MetaCyc:MONOMER0-2682
UniPathwayiUPA00030

Protein family/group databases

TCDBi2.A.7.22.1 the drug/metabolite transporter (dmt) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE
Short name:
L-Ara4N-phosphoundecaprenol flippase subunit ArnE
Alternative name(s):
Undecaprenyl phosphate-aminoarabinose flippase subunit ArnE
Gene namesi
Name:arnE
Synonyms:pmrL, yfbW
Ordered Locus Names:b4544, JW2252
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14344 arnE

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 35CytoplasmicSequence analysisAdd BLAST35
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Topological domaini57 – 61PeriplasmicSequence analysis5
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Topological domaini83 – 87CytoplasmicSequence analysis5
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Topological domaini109 – 111PeriplasmicSequence analysis3

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene lack L-Ara4N-modified lipid A and are sensitive to polymyxin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001691841 – 111Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnEAdd BLAST111

Proteomic databases

PaxDbiQ47377
PRIDEiQ47377

Interactioni

Subunit structurei

Heterodimer of ArnE and ArnF.1 Publication

Protein-protein interaction databases

BioGridi4260500, 209 interactors
STRINGi316385.ECDH10B_2418

Structurei

3D structure databases

ProteinModelPortaliQ47377
SMRiQ47377
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 109EamAAdd BLAST70

Sequence similaritiesi

Belongs to the ArnE family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41060GT Bacteria
COG0697 LUCA
HOGENOMiHOG000261165
InParanoidiQ47377
KOiK12962
OMAiTCAGQLC

Family and domain databases

HAMAPiMF_01869 Flippase_ArnE, 1 hit
InterProiView protein in InterPro
IPR000620 EamA_dom
IPR022883 Flippase_ArnE
PfamiView protein in Pfam
PF00892 EamA, 1 hit

Sequencei

Sequence statusi: Complete.

Q47377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIWLTLVFAS LLSVAGQLCQ KQATCFVAIN KRRKHIVLWL GLALACLGLA
60 70 80 90 100
MVLWLLVLQN VPVGIAYPML SLNFVWVTLA AVKLWHEPVS PRHWCGVAFI
110
IGGIVILGST V
Length:111
Mass (Da):12,192
Last modified:April 27, 2001 - v2
Checksum:i7CFA06D75DA33D69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: ABD18693.1
AP009048 Genomic DNA Translation: BAE76675.1
L35031 Genomic DNA Translation: AAB04895.1
RefSeqiWP_000638031.1, NZ_LN832404.1
YP_588462.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiABD18693; ABD18693; b4544
BAE76675; BAE76675; BAE76675
GeneIDi1450282
KEGGiecj:JW2252
eco:b4544
PATRICifig|1411691.4.peg.4479

Similar proteinsi

Entry informationi

Entry nameiARNE_ECOLI
AccessioniPrimary (citable) accession number: Q47377
Secondary accession number(s): Q2EES8, Q2MAN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: March 28, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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